...ahcyl1 TAL3018 & TAL3019 TGACAGACTGTGGCGAGGatgccaagctggaggcGACGAAACAGGCCAGCAA amh TAL3210 & TAL3211 TCCAGGCAAGATTTGGGCtgatgctgatgatgacCGTGGCGATTGGGTCGTA... apoa4 TAL3022 & TAL3025 TGGCAGGACCAACCAATGcccagcatggacctggTGAAAAATGCTTTCTGGA apoa4-like TAL3023 & TAL3024...TAL3024 TGAAGGTTCTTGTGGTGCtcacacttgctgtggtTACAGGTAAGAAACTAAA apoeb TAL3402 & TAL3403 TGCCAGGCTCGTAGCCTGttccaggctgatgccccTCAGCCCAGATGGGAGGA... asun TAL3404 & TAL3405 TAACTCAAGCCAACTCCAggaaacatcaggagtttGCAGGAAGATTGAGTTCA atf6 TAL3406 & TAL3407 TCCAACCAGCAAAAAGACccatccaggtgtgccccaAGGTTTCCATTCAGCCTA... TCCAACCAGCAAAAAGACccatccaggtgtgccccaAGGTTTCCATTCAGCCTA atg5 TAL3218 & TAL3219 TAAAGTCAAGAAACATTTtctcaaagtcatgaagGCAGAGGATGTGGAGGAA...TCAGCTCCCCGGAGAGGCaccgcactgctccagggTTGGATGATGGACATGGA bsx TAL3034 & TAL3035 TCTCAGACTCACAGTTATccggactcgagaaaagGTTCGAGATCCAGAGATA btr30 TAL3036 & TAL3037 ...casp8ap2 TAL3038 & TAL3039 TATAACAAATCCCACCACattgcccgatatcttgAATACTTCCAAAACAAAA Ccl2 TAL3222 & TAL3223 TTTTCGTTTTCCACTATAaagatcccagttaagcAGGTTCAGAGTTATCATA...

...thaliana TTTGAAAGATGGAAGCGCGG cut S. pyogenes 26479191 Kim BRI1 A. thaliana TGAAACTAAACTGGTCCACA cut S. ...Species PubMed ID Depositor OCT4 H. sapiens CTCCCATGCATTCAAACTG 66989 cut S. pyogenes 26028531 Huangfu OCT4... pyogenes 26541286 Voytas APC H. sapiens GAAGCGGGCAAAGGGGCGAC 58780 cut/nick S. pyogenes 24954249 Yamamoto...pyogenes 25619936 Sato ASCL1 H. sapiens TGGGGCTGGGTGTCCCATTGAAA 64129 activate S. pyogenes 25619936 Sato...pyogenes 24967838 Mashimo ATF1 H. sapiens TAGGAATCAAACACTTTTATTGG 64690 tag S. pyogenes 26355004 Mendenhall...pyogenes 24954249 Yamamoto BLIMP1 H. sapiens CGGATGGGGTAAACGACCCG 59724 cut S. pyogenes 25543152 Hanna BT1754...BT1754 B. thetaiotaomicron GAAAATGGGGTGTATCCTGC 68892 interfere S. pyogenes 26918244 Lu BT1854 B. thetaiotaomicron...

...) ABL1 TAL2380 & TAL2381 TACCTATTATTACTTTATggggcagcagcctggaaAAGTACTTGGGGACCAA AKT2 TAL2382 & TAL2383 TGTGTCTTGGGATGAGTGggtcagtgttctggtgCTCACAGGATGGCTGGCA...TATGTACGCCTCCCTGGGctcgggtccggtcgccCCTTTGCCCGCTTCTGTA ATM TAL2402 & TAL2403 TGAATTGGGATGCTGTTTttaggtattctattcaaaTTTATTTTACTGTCTTTA AXIN2 TAL2200 & TAL2201...TCCCTCACCATGAGTAGCgctatgttggtgacttGCCTCCCGGACCCCAGCA BAX TAL2296 & TAL2297 TGTGCGATCTCCAAGCACtgaggggcagaaactcCCGGATCGGGCGCTGCCA BCL6 TAL2412 & TAL2413 TTTTCAAGTGAAGACAAAatggcctcgccggctgACAGCTGTATCCAGTTCA... TTTTCAAGTGAAGACAAAatggcctcgccggctgACAGCTGTATCCAGTTCA BMPR1A TAL2260 & TAL2261 TACAATTGAACAATGCCTcagctatacatttacatCAGATTATTGGGAGCCTA... BRCA2 TAL2302 & TAL2303 TTAGACTTAGGTAAGTAAtgcaatatggtagactGGGGAGAACTACAAACTA CBX3 TAL2358 & TAL2359 TCTGCAATAAAAAATGGCctccaacaaaactacaTTGGTAAGTTAATGAAAA... TCTGCAATAAAAAATGGCctccaacaaaactacaTTGGTAAGTTAATGAAAA CBX8 TAL2360 & TAL2361 TGGAGCTTTCAGCGGTGGgggagcgggtgttcgcgGCCGAAGCCCTCCTGAA...CCND1 TAL2304 & TAL2473 TGGAACACCAGCTCCTGTgctgcgaagtggaaaccatCCGCCGCGCGTACCCCGA CDC73 TAL2202 & TAL2203...

...gCCCCTCAGCccagatgGGAGGAGATg dpf2 OZ531 and OZ532 OPEN OPEN cTGCTACTTCtggatGGAGAAACGa pitx2 OZ533 and OZ534 OPEN OPEN cCGCTTCAGCggtctGTGGACTCGa...Encephalopsin (Opn3) OZ541 and OZ542 OPEN OPEN aTCCATCCCAaacacatGTGGCAGAAt NFATc1 OZ543 and OZ544 OPEN OPEN aCCTCGCCTCagtgtGACGGAGGAc...Gene (sbds) OZ545 and OZ546 OPEN OPEN tGTTGCCGTCgtgagGATGAAGAAa CACNA1D (2of2) or cav1.3b OZ547 and OZ548... H3 (hrh3) OZ549 and OZ550 OPEN OPEN gCGCCACGGAcaataaaGAGGACTGCa GUSB OZ551 and OZ552 OPEN OPEN gGTCATCTGCcattcGAGGCGGACa...tTACCTCCACGATCTTGCTGATGGAg dio2 OZ569 and OZ570 CODA OPEN aAGCTGCTCCAAACTCTAAGGTGGTg im:7163548 OZ571 and OZ572 CODA CODA ...001003579.1) OZ575 and OZ576 CODA OPEN cAACCTCAGCATAAAGCTGGAGGAg psen2 OZ577 and OZ578 CODA OPEN cGGCATCTCCAGAGCCCGAGGCGGACc...A4QNT7_DANRE) OZ585 and OZ586 CODA CODA cTGCACCTTCACAAAAGGCCGCTGGCt LOC566040 OZ587 and OZ588 OPEN OPEN cAGCACCAGCCAGCCCAGAGTTGGGGt...

...expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol 48651 PM-NM!TA Bacterial...expression: targets NM to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol 48653 PM-ST1!TA Bacterial...expression: targets ST1 to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol 48655 PM-TD!TA Bacterial...expression: targets TD to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol 48657 DS-SPcasN- ...

...Capecchi 137860 pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAA) iCre-ERT2 (PAAA N-terminus) - Tamoxifen inducible CAG Mammalian...Capecchi 137861 pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAAA) iCre-ERT2 (PAAAA N-terminus) - Tamoxifen inducible CAG Mammalian...

...GIGYF2 GST tac Parkinson's Sachdev Sidhu 109964 pHH0103_VCP_AAA VCP GST tac ALS Sachdev Sidhu 110028 pHH0103...Elisa Izaurralde 148521 pT7-EGFP-C1-HseIF4G1-LIVVtoAAAA_AD EIF4G GFP CMV Parkinson's Elisa Izaurralde 148522...Izaurralde 148523 pT7-EGFP-C1-HseIF4G_615-654-LIVVtoAAAA_AD EIF4G GFP CMV Parkinson's Elisa Izaurralde 148524...Izaurralde 148580 pT7-EGFP-C1-HsGIGYF2_1-180-YYMLtoAAAA_AE GIGYF2 GFP CMV Parkinson's Elisa Izaurralde ...Elisa Izaurralde 148691 pT7-EGFP-C1-HsGIGYF2-YYMLtoAAAA_AF GIGYF2 GFP CMV Parkinson's Elisa Izaurralde ...Izaurralde 148950 pT7-EGFP-C1-HsGIGYF2_1-718-YFWFtoAAAA_AI GIGYF2 GFP CMV Parkinson's Elisa Izaurralde ...

... is: 5'-CACCGN...N-3' 3'-CN...NCAAA-5' You need to add the CACC and AAAC cloning overhang. When designing...

...Add “CACC” before the 20-mer guide sequence and “AAAC” before the guide’s reverse complement for cloning...using a U6 promoter forward primer: CGTAACTTGAAAGTATTTCGATTTCTTGGC. This is a representative sequencing...

...TGGCCGTTTACGTCGCCG eGFP-TALEN-37-Left TAL2096 TAAACGGCCACAAGTTCA eGFP-TALEN-37-Right TAL2097 TAGGTGGCATCGCCCTCG...

...thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s from various species PAM sequence...