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We narrowed to 223 results for: antibody

Showing: 21 - 40 of 223 results

  1. Antibodies 101: ChIP

    Type
    Blog Post
    ...s blog Antibodies 101: Immunoprecipitation  Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction...DNA Incubate your antibody, or antibodies, with your samples, and then use an antibody capture method such... ChIP works by using antibodies to capture protein:DNA complexes with antibodies specific to your protein...mask the epitopes antibodies recognize. It is therefore best to either select antibodies validated for ChIP...incubate the sample with your antibody.  Both polyclonal and monoclonal antibodies can work well in ChIP. If...Introduction to Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene... ChIP or to validate your antibody on crosslinked proteins. In the case of histone modifying proteins,...

  2. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ... the same basic strategy: immobilize the antibody or antibody-target protein immune complexes, wash away... this method, the free antibody is incubated with the protein mix and antibody-target protein immune complexes... the process of anchoring an antibody, often called the capture antibody, to agarose or magnetic beads...primary antibody has the additional benefit of permanent linkage. This means that the primary antibody will...primary antibody interfering with downstream assays. This is particularly advantageous if the antibody used...learn more about antibodies here!) When using Protein A/G to immobilize the antibody, it is recommended... using a nonspecific antibody from the same host species as the capture antibody. This will deplete the...

  3. Antibodies 101: Isotypes

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    Blog Post
    ...region (see Antibodies 101: Introduction to Antibodies for a refresher). There are five antibody isotypes...Intro to Antibodies post Check out our Antibodies 101: What is an Antibody animation  Resources on Addgene.org...heard of an antibody isotype? An isotype determines several key characteristics of an antibody as well as...And how do antibodies switch isotypes? What is an isotype? An isotype is a class of antibody that’s determined...region.   Why are antibody isotypes important? To your immune system, an antibody’s isotype is important...Transportation of antibodies to places they can’t reach on their own. The antibody’s heavy-chain constant...milk. When using antibodies in the lab, it’s useful to know the isotype of an antibody so you can select...

  4. Antibody Guide

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    Collection
    ...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production... produce antibodies, with the highest variability in antibodies produced. Cells - Antibodies can also ...called recombinant antibodies. Recombinant antibodies are created by cloning antibody genes from specific... in a buffer. Antibody Structure Figure 1: Structure of an antibody A standard antibody is made up of ...specificity in antibody-based methods. Antibodies can recognize and bind other antibodies using epitopes...per antibody unit, which is typically the number of antibodies multiplied by two, as each antibody has...These are antibody mimics instead of modified antibodies and are not closely related to antibodies. Monobodies...

  5. Antibody Production

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    Collection
    ...Antibodies Antibody Production Antibody Production Many antibodies distributed by Addgene have been produced... use your antibody. Antibody Blog Posts Recipient Instructions Addgene Help Center Antibody Protocols ...Addgene's antibody production methods and quality control processes for purified recombinant antibodies. Antibodies...specific antibody in order to generate high quality preparations. Following production, all antibody preps...below. Information for a specific antibody can be found on the antibody's catalog page or by contacting help...IPI). For these antibodies, IPI will be indicated as the Depositing Lab on the antibody webpage. To learn...more about these antibodies, please visit the Institute for Protein Innovation Antibody Collection webpage...

  6. Antibody Plasmid Collection

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    Collection
    ...Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large proteins made by the ...about antibodies and their applications in our Antibody Guide ! Cloning and Expressing Antibodies The following... collection of plasmid-based antibodies, including monoclonal antibodies, scFvs, and nanobodies. Plasmid...Plasmid Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-...recombinant antibodies made from plasmids in our collection! Many of these monoclonal antibodies are produced... intruders, antibodies are also extremely useful to researchers in a lab. When an antibody binds tightly...synthetically produced recombinant antibodies, created by cloning antibody components into plasmids and expressing...

  7. All Antibodies

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    Collection
    ... Antibodies All Antibodies All Antibodies Addgene distributes ready-to-use...and secondary antibodies. To learn more about antibodies and their use, visit our Antibody Guide . Browse...new antibodies to add to the collection using our Antibody Suggestion Form . Browse All Antibodies ID ... use your antibody. Antibody Blog Posts Recipient Instructions Addgene Help Center Antibody Protocols ...ready-to-use recombinant antibodies. These monoclonal antibodies undergo application-specific validation...plasmids we use to produce antibodies are available as part of Addgene’s larger Antibody Plasmid Collection ,...pages to find antibodies in our curated collections: Tags and Other Markers : Antibodies targeting popular...

  8. Antibodies 101: Choosing the Right Isotype

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    Blog Post
    ...Addgene The Antibody Guide Antibodies 101: Isotypes Antibodies 101: Choosing the Right Antibody  ...polyclonal antibody is better for your experiment. Monoclonal antibodies contain a single antibody clone and...IgG2a antibody. When using a monoclonal antibody be sure to use a highly specific secondary antibody, rather... secondary antibody depends on whether you are using poly- or monoclonal primary antibodies. Some secondary...anti-mouse antibody. Others recognize specific isotopes within that species, like an anti-mouse IgG antibody. Some...In a broadly specific secondary antibody only a fraction of the antibody molecules will recognize and bind...primary antibody. Manning et al. tested a variety of commercially available secondary antibodies and found...

  9. Institute for Protein Innovation

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    Collection
    ...Innovation Antibody Collection Glypican Antibodies Integrin Antibodies Epitope Tag Antibodies Antibody Plasmids...well as antibody plasmids to create or express plasmid-based antibodies. The majority of antibodies in the... Antibodies Institute for Protein Innovation Antibody Collection Institute for...Figure courtesy of IPI Browse Glypican Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...Figure courtesy of IPI Browse Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...Browse Epitope Tag Antibodies IPI has developed recombinant versions of antibodies that recognize common... Other Markers Antibody Collection page to browse Addgene’s full catalog of antibodies that target epitope...

  10. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    Blog Post
    ...monoclonal antibodies as well as other forms of antibody-based (e.g., nanobodies, ScFVs) and non-antibody based...Recombinant Antibodies Recombinant antibodies offer numerous advantages over polyclonal antibodies and traditional...encoding antibodies, scientists will be able to make higher affinity antibodies, modify antibody function...I predict a similar revolution for antibodies. Recombinant antibody technology makes it easy for scientists...scientific toolbox. What are Recombinant Antibodies? Antibodies are used by scientists around the world...Top) Polyclonal antibodies are produced in animals and consist of a mixture of antibodies recognizing many...Middle) Monoclonal antibodies produced from hybridomas are usually a single antibody recognizing one epitope...

  11. Antibodies 101: The Four ELISAs and When to Use Them

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    Blog Post
    ...Addgene blog Antibodies 101: ELISAsAntibodies 101: Selecting the Right Antibody Antibodies 101: Polyclonal...unconjugated primary antibody and a conjugated secondary antibody. Incorporating a secondary antibody increases ...antigen-specific detection antibody.  Importantly, the detection antibody and the capture antibody must bind to distinct...detection antibodies or unconjugated detection antibodies followed by conjugated secondary antibodies. Assay...Polyclonal Antibodies Resources on addgene.org The Addgene Antibody GuideAddgene's recombinant antibody collection...target antigen binds to antigen-specific antibodies. The antibodies are conjugated with a reporter or tag...primary antibody first binds to the antigen and then a reporter enzyme conjugated secondary antibody binds...

  12. Corralling and Cornering the Goldilocks Antibody

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    Blog Post
    ...and cost-effective) antibody for my experiment – the “goldilocks antibody.” Antibodies are one of the most... the antibody crisis, one must corral and corner the goldilocks antibodies. A goldilocks antibody is highly... blog Antibodies 101: ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ... popular and useful antibodies are, they are in a midst of a crisis. The “antibody crisis” came to light...light as antibodies became commercialized, revealing the unreliability of antibodies. There are three main...ability – i.e., the antibody can no longer bind to the target). Unreliable antibodies cause research that...explaining the antibody crisis states that up to 50% of all commercially available antibodies are unreliable...

  13. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post
    ...each antibody performs differently and some antibodies impact the performance of other antibodies. But...researchers use a single antibody to visualize their target. These antibodies are generated against a ... requires two antibodies: a primary and secondary. For indirect IF, the primary antibody targets the antigen...secondary antibodies on hand to mix and match with a larger collection of primary antibodies. This strategy...target and sample type. Primary Antibody This is the first antibody applied to your sample, which will...incubation time. Secondary Antibody Unsurprisingly, this is the second antibody you apply, which binds ...experimental context.  Choosing antibodies for your assay The antibodies you choose for your experiment...

  14. A Control for All Seasons

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    Blog Post
    ...Recommended controls for antibody-based experiments Primary antibody specificity Antibody-based assays have ...Microscopy-based antibody experiments should include a primary antibody specificity control, secondary antibody specificity...Addgene blog Antibodies 101: Validation Antibodies 101: Choosing the Right Isotype Antibodies 101: Selecting...confirm primary antibody specificity. The gold standard in demonstrating primary antibody specificity is...approaches discussed in the Antibodies 101: Validation post. The primary antibody control needs only to be...standard to demonstrate primary antibody specificity. B, secondary antibody-only staining will allow you ...secondary antibody. C, a label control treats the sample to the full protocol without any antibodies to determine...

  15. Antibody Validation for Flow Cytometry

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    Blog Post
    ...Synaptotagmin-1 antibodies and corresponding Multi-rAb CoraLite® Plus 647 secondary antibodies. Antibody staining...multiple antibody clones targeting the same protein (independent antibodies). If two antibodies, that recognize...Antibody validation is to confirm (or refute) that the antibody is selectively detecting the target-of-interest...pillars of antibody validation, and it is recommended to screen multiple candidate antibodies when possible...monoclonal antibodies. It is also important to seek out supportive data on candidate antibodies from the...stained with secondary antibodies, showing the level of background staining. All antibodies were diluted to ...0.35 µg/mL (1/100). * = monoclonal antibody, ** = recombinant antibody. Figure adapted from Biddle et al...

  16. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...which antibody to request for your experiment!  Addgene’s Antibody Data Hub Addgene’s Antibody Data Hub...Updated AAV Data Hub Antibodies 101: Validation Antibodies 101: Selecting the Right Antibody...the Addgene antibody collection can look at user-deposited data detailing how an antibody performed in...Figure 2: Search options in the Antibody Data Hub. Data reports  Each antibody submission is used to create... the antibody performed.  Figure 5: Results for the data report shown in Figure 3. The antibody is rated...requesting an Addgene antibody, the Data Hub can help you decide if an antibody is a good candidate for...and used an Addgene antibody, we encourage you to submit data showing how the antibody worked in your application...

  17. Addgene Begins Distribution of Recombinant Antibodies

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    Blog Post
    ...ready-to-use antibodies for your experiments directly from Addgene. How Addgene is Reimagining Antibody Sharing...Addgene is building our antibody resource, which includes recombinant antibodies, educational materials...RRIDs) for each antibody, making it easy for you to determine whether any given antibody is available through...Addgene’s antibodies are plasmid-based and recombinant. Learn more about recombinant antibodies here. Finally...Will Start With Neuroscience Antibodies and Grow From There Addgene’s antibody resource is funded by a generous... Figure 1: Antibody against PSD-95 from the Trimmer Lab. Information about the antibody, the target ...accompany our antibody collection.  Addgene is sharing our protocols for recombinant antibody production ...

  18. Antibodies 101: Fab Fragments

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    Blog Post
    ...regions of the antibody that actually bind antigens.      Figure 1: IgG antibody structure with...Fab portion of the antibody. Fab fragments are useful when you just need an antibody’s antigen binding function...   Why Fabs?  Antibody fragments have a handful of advantages over full antibodies. Their smaller size... use Fab fragments as primary antibodies, you can make your own antibody fragments as needed.  In contrast...contrast, anti-IgG antibodies, commonly used as secondary antibodies, have much broader applicability. It...secondary antibody recognizes endogenous antigens. In these cases, even though one full antibody may be ...two full antibodies bound together may be too large. Using a complex that is one full antibody bound to...

  19. Antibodies 101: Fc Effector Functions

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    Blog Post
    ...secondary antibodies to bind. We also know that changing the Fc domain can impact an antibody's binding ... affect antibody applications like ELISAs or Western blots, they are key in helping antibodies drive an...responses? We got you! You can also review antibodies in our Antibodies 101 animation.)  Many types of immune...a viral infection a) antibody-dependent complement deposition (ADCD) b) antibody-dependent cellular cytotoxicity... et al., 2019.    Antibody-dependent complement deposition (ADCD) Antibodies can bind to a pathogen...opsonization. In antibody-dependent complement deposition (ADCD), opsonization of IgG and IgM antibodies on a pathogen...quickly lyse and die.  Antibody dependent cellular cytotoxicity (ADCC) Antibody dependent cellular cytotoxicity...

  20. Antibodies 101: Affinity Reagents

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    Blog Post
    ...from camelid antibodies to antibody fragments to tools completely unrelated to antibodies.  Why use an... are eliminated, as is antibody recognition, since antibody-specific antibodies bind to and recognize ... (the antibody region that binds to the antigen) from existing antibodies. Each cleaved antibody will ....org  Antibody GuideRecombinant antibodies available from AddgeneBrowse our recombinant antibody plasmid...101: Aptamer FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction to Antibodies Additional resources...Addgene's new Antibody Guide and not just because I helped create it. But while antibodies, as the OG (...co-crystalize, a major advantage over antibodies (Kwon, 2019).  Single-domain antibodies (sdAbs), sold under the commercial...
Showing: 21 - 40 of 223 results