We narrowed to 232 results for: antibody

This protocol describes the basic steps for lysing cells, determining total protein concentration in the lysate, running a precast SDS-PAGE gel, and immunoblotting.

Lab protocol for transfection of HEK293 cells to produce recombinant antibody.

Lab protocol to determine purity and concentration of recombinant antibodies using ready-to-use biosafe Coomassie stain and ImageJ software.

This protocol describes how to affinity purify recombinant antibodies from cell culture supernatant using Protein A or Protein G columns.

A wide variety of protocols from Addgene that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations.

Find unique recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration between Addgene and IPI.

Browse Addgene's collection of recombinant monoclonal antibodies.

Overview of Addgene's ready-to-use monoclonal recombinant antibody collection. These affinity reagents are expressed and purified from plasmids in our repository.

Find recombinant antibodies targeting epitope tags and common fusion proteins.

Find recombinant antibodies targeting neuroscience-related proteins.

The NeuroMab Facility and James Trimmer have produced monoclonal antibodies validated for neuroscience research. Find plasmids encoding these R-mAbs here.

Antibody validation shows that your antibody is targeting exactly what it is supposed to. This post walks through 5 validation strategies.

When you have multiple antibodies to choose from, does isotype matter? Yes, and understanding how can help you pick the right antibodies for your experiment.

An overview of secondary antibodies and the indirect approach in immunoimaging assays.

Polyclonal antibodies are a common research reagent - learn how they’re produced and the advantages and disadvantages of using them in your next experiment.

Which ELISA is right for your experiment? Read our blog post to help you decide!

Right variable region, but wrong constant? Chimeric antibodies may be the answer!

Everything you need to know about antibodies in this blog post! Get the facts about the types of antibodies and how they can be used in the lab.

Plasmid-encoded antibodies offers many advantages over other types of antibodies. Learn about how recombinant antibodies are made.

Get the basics on monoclonal antibodies including how monoclonal antibodies are made and why use monoclonal antibodies for your experiment.