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We narrowed to 224 results for: antibody

Showing: 21 - 40 of 224 results

  1. Antibodies 101: Isotypes

    Type
    Blog Post
    ...region (see Antibodies 101: Introduction to Antibodies for a refresher). There are five antibody isotypes...Intro to Antibodies post Check out our Antibodies 101: What is an Antibody animation  Resources on Addgene.org...heard of an antibody isotype? An isotype determines several key characteristics of an antibody as well as...And how do antibodies switch isotypes? What is an isotype? An isotype is a class of antibody that’s determined...region.   Why are antibody isotypes important? To your immune system, an antibody’s isotype is important...Transportation of antibodies to places they can’t reach on their own. The antibody’s heavy-chain constant...milk. When using antibodies in the lab, it’s useful to know the isotype of an antibody so you can select...

  2. New Antibody Animation!

    Type
    Blog Post
    ...about antibodies? Our latest animation is here to help! Join Abi as they explain what antibodies are, ...role in the immune response, and the different antibody isotypes found in mammals.      Subscribe to...

  3. Antibodies 101: Conjugation

    Type
    Blog Post
    ... the methods for antibody conjugation can be quite variable from antibody to antibody or batch to batch...research antibodies. Lysine residues are found throughout antibody sequences, up to 80 per antibody (Mueller... conjugate antibodies directly to beads for purification purposes.  Coupling up - antibody conjugation...disulfide bonds between antibody chains. 3) Endogenous carbohydrates added to antibodies post-translationally...commercially conjugated antibodies available, that never guarantees the antibody you need will be conjugated... conjugate an antibody yourself allows you to consider a broader selection of antibodies for your experiments...confirm your antibodies’ performance after the reaction.  If you are looking to conjugate antibodies yourself...

  4. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...Addgene The Antibody Guide Antibodies 101: Isotypes Antibodies 101: Choosing the Right Antibody  ...polyclonal antibody is better for your experiment. Monoclonal antibodies contain a single antibody clone and...IgG2a antibody. When using a monoclonal antibody be sure to use a highly specific secondary antibody, rather... secondary antibody depends on whether you are using poly- or monoclonal primary antibodies. Some secondary...anti-mouse antibody. Others recognize specific isotopes within that species, like an anti-mouse IgG antibody. Some...In a broadly specific secondary antibody only a fraction of the antibody molecules will recognize and bind...primary antibody. Manning et al. tested a variety of commercially available secondary antibodies and found...

  5. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    Blog Post
    ...monoclonal antibodies as well as other forms of antibody-based (e.g., nanobodies, ScFVs) and non-antibody based...Recombinant Antibodies Recombinant antibodies offer numerous advantages over polyclonal antibodies and traditional...encoding antibodies, scientists will be able to make higher affinity antibodies, modify antibody function...I predict a similar revolution for antibodies. Recombinant antibody technology makes it easy for scientists...scientific toolbox. What are Recombinant Antibodies? Antibodies are used by scientists around the world...Top) Polyclonal antibodies are produced in animals and consist of a mixture of antibodies recognizing many...Middle) Monoclonal antibodies produced from hybridomas are usually a single antibody recognizing one epitope...

  6. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...Addgene blog Antibodies 101: ELISAsAntibodies 101: Selecting the Right Antibody Antibodies 101: Polyclonal...unconjugated primary antibody and a conjugated secondary antibody. Incorporating a secondary antibody increases ...antigen-specific detection antibody.  Importantly, the detection antibody and the capture antibody must bind to distinct...detection antibodies or unconjugated detection antibodies followed by conjugated secondary antibodies. Assay...Polyclonal Antibodies Resources on addgene.org The Addgene Antibody GuideAddgene's recombinant antibody collection...target antigen binds to antigen-specific antibodies. The antibodies are conjugated with a reporter or tag...primary antibody first binds to the antigen and then a reporter enzyme conjugated secondary antibody binds...

  7. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...and cost-effective) antibody for my experiment – the “goldilocks antibody.” Antibodies are one of the most... the antibody crisis, one must corral and corner the goldilocks antibodies. A goldilocks antibody is highly... blog Antibodies 101: ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ... popular and useful antibodies are, they are in a midst of a crisis. The “antibody crisis” came to light...light as antibodies became commercialized, revealing the unreliability of antibodies. There are three main...ability – i.e., the antibody can no longer bind to the target). Unreliable antibodies cause research that...explaining the antibody crisis states that up to 50% of all commercially available antibodies are unreliable...

  8. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post
    ...each antibody performs differently and some antibodies impact the performance of other antibodies. But...researchers use a single antibody to visualize their target. These antibodies are generated against a ... requires two antibodies: a primary and secondary. For indirect IF, the primary antibody targets the antigen...secondary antibodies on hand to mix and match with a larger collection of primary antibodies. This strategy...target and sample type. Primary Antibody This is the first antibody applied to your sample, which will...incubation time. Secondary Antibody Unsurprisingly, this is the second antibody you apply, which binds ...experimental context.  Choosing antibodies for your assay The antibodies you choose for your experiment...

  9. A Control for All Seasons

    Type
    Blog Post
    ...Recommended controls for antibody-based experiments Primary antibody specificity Antibody-based assays have ...Microscopy-based antibody experiments should include a primary antibody specificity control, secondary antibody specificity...Addgene blog Antibodies 101: Validation Antibodies 101: Choosing the Right Isotype Antibodies 101: Selecting...confirm primary antibody specificity. The gold standard in demonstrating primary antibody specificity is...approaches discussed in the Antibodies 101: Validation post. The primary antibody control needs only to be...standard to demonstrate primary antibody specificity. B, secondary antibody-only staining will allow you ...secondary antibody. C, a label control treats the sample to the full protocol without any antibodies to determine...

  10. Antibody Validation for Flow Cytometry

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    Blog Post
    ...Synaptotagmin-1 antibodies and corresponding Multi-rAb CoraLite® Plus 647 secondary antibodies. Antibody staining...multiple antibody clones targeting the same protein (independent antibodies). If two antibodies, that recognize...Antibody validation is to confirm (or refute) that the antibody is selectively detecting the target-of-interest...pillars of antibody validation, and it is recommended to screen multiple candidate antibodies when possible...monoclonal antibodies. It is also important to seek out supportive data on candidate antibodies from the...stained with secondary antibodies, showing the level of background staining. All antibodies were diluted to ...0.35 µg/mL (1/100). * = monoclonal antibody, ** = recombinant antibody. Figure adapted from Biddle et al...

  11. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...which antibody to request for your experiment!  Addgene’s Antibody Data Hub Addgene’s Antibody Data Hub...Updated AAV Data Hub Antibodies 101: Validation Antibodies 101: Selecting the Right Antibody...the Addgene antibody collection can look at user-deposited data detailing how an antibody performed in...Figure 2: Search options in the Antibody Data Hub. Data reports  Each antibody submission is used to create... the antibody performed.  Figure 5: Results for the data report shown in Figure 3. The antibody is rated...requesting an Addgene antibody, the Data Hub can help you decide if an antibody is a good candidate for...and used an Addgene antibody, we encourage you to submit data showing how the antibody worked in your application...

  12. Antibody Guide

    Type
    Collection
    ...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production... produce antibodies, with the highest variability in antibodies produced. Cells - Antibodies can also ...called recombinant antibodies. Recombinant antibodies are created by cloning antibody genes from specific... in a buffer. Antibody Structure Figure 1: Structure of an antibody A standard antibody is made up of ...specificity in antibody-based methods. Antibodies can recognize and bind other antibodies using epitopes...per antibody unit, which is typically the number of antibodies multiplied by two, as each antibody has...These are antibody mimics instead of modified antibodies and are not closely related to antibodies. Monobodies...

  13. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...ready-to-use antibodies for your experiments directly from Addgene. How Addgene is Reimagining Antibody Sharing...Addgene is building our antibody resource, which includes recombinant antibodies, educational materials...RRIDs) for each antibody, making it easy for you to determine whether any given antibody is available through...Addgene’s antibodies are plasmid-based and recombinant. Learn more about recombinant antibodies here. Finally...Will Start With Neuroscience Antibodies and Grow From There Addgene’s antibody resource is funded by a generous... Figure 1: Antibody against PSD-95 from the Trimmer Lab. Information about the antibody, the target ...accompany our antibody collection.  Addgene is sharing our protocols for recombinant antibody production ...

  14. Antibodies 101: Fab Fragments

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    Blog Post
    ...regions of the antibody that actually bind antigens.      Figure 1: IgG antibody structure with...Fab portion of the antibody. Fab fragments are useful when you just need an antibody’s antigen binding function...   Why Fabs?  Antibody fragments have a handful of advantages over full antibodies. Their smaller size... use Fab fragments as primary antibodies, you can make your own antibody fragments as needed.  In contrast...contrast, anti-IgG antibodies, commonly used as secondary antibodies, have much broader applicability. It...secondary antibody recognizes endogenous antigens. In these cases, even though one full antibody may be ...two full antibodies bound together may be too large. Using a complex that is one full antibody bound to...

  15. Antibodies 101: Fc Effector Functions

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    Blog Post
    ...secondary antibodies to bind. We also know that changing the Fc domain can impact an antibody's binding ... affect antibody applications like ELISAs or Western blots, they are key in helping antibodies drive an...responses? We got you! You can also review antibodies in our Antibodies 101 animation.)  Many types of immune...a viral infection a) antibody-dependent complement deposition (ADCD) b) antibody-dependent cellular cytotoxicity... et al., 2019.    Antibody-dependent complement deposition (ADCD) Antibodies can bind to a pathogen...opsonization. In antibody-dependent complement deposition (ADCD), opsonization of IgG and IgM antibodies on a pathogen...quickly lyse and die.  Antibody dependent cellular cytotoxicity (ADCC) Antibody dependent cellular cytotoxicity...

  16. Antibodies 101: Affinity Reagents

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    Blog Post
    ...from camelid antibodies to antibody fragments to tools completely unrelated to antibodies.  Why use an... are eliminated, as is antibody recognition, since antibody-specific antibodies bind to and recognize ... (the antibody region that binds to the antigen) from existing antibodies. Each cleaved antibody will ....org  Antibody GuideRecombinant antibodies available from AddgeneBrowse our recombinant antibody plasmid...101: Aptamer FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction to Antibodies Additional resources...Addgene's new Antibody Guide and not just because I helped create it. But while antibodies, as the OG (...co-crystalize, a major advantage over antibodies (Kwon, 2019).  Single-domain antibodies (sdAbs), sold under the commercial...

  17. Antibodies 101: Epitope Availability

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    Blog Post
    ...s blog Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction...collection of antibodies in your lab. Perhaps you’re even in that mythical place where antibodies are well-...hides the epitope that the antibodies used to recognize the protein, your antibody will no longer be effective...monoclonal antibodies that have been validated solely for denaturing assays. Many antibodies will note ...Validation  Once you’ve selected an antibody, or multiple antibodies, you’ll need to validate them for ...which antibodies are suitable for which assays and conditions when you’re building your antibody library...Introduction to Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene...

  18. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...multiple secondary antibodies can bind to a single primary antibody. The secondary antibody is conjugated ... coated with the antibody. Then, you add your sample to the wells, and the antibodies bind to your protein...is the addition of a detection antibody (typically a monoclonal antibody) that also recognizes the same...same target protein. The capture antibody and the detection antibody recognize different epitopes on the... to that antibody. Then, the protein-antigen mixture is added to the wells. Any free antibody (meaning...two different antibodies. As mentioned above, it can also be used to characterize antibodies against the...using monoclonal antibodies. Read about other techniques that you can use antibodies for in our Intro ...

  19. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...use two antibodies - one against your target of interest and a second against the first antibody. When ...same species as your primary antibody are present, your secondary antibody recognizes both the endogenous...because the secondary antibody is not able to distinguish between the primary antibody and endogenous IgGs...secondary antibody bind endogenous IgGs? Don’t use one. If it is possible, using a primary antibody that is...samples as homogeneously as a single antibody. Using a secondary antibody that is a Fab fragment, single-chain... and secondary antibodies before adding them to your sample so the secondary antibodies aren’t free to...on the Addgene blog Antibodies 101: Introduction to Immunofluorescence Antibodies 101: Affinity Reagents...

  20. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...smaller antibodies.They are derived from an unusual type of IgG antibody called a heavy chain antibody (HCab...require secondary antibodies, frequently of the mouse or rabbit variety. While antibodies certainly aren’...Nanobodies are like tiny antibodies which work just as well, if not better, than antibodies for all of the above...traditional IgG antibody structures. Functional comparison Strong but Large: Advantages of Antibodies One of ...different epitopes of a primary antibody. B) Each arm of the antibody has an antigen binding site for ...be attached to each antibody. But being big is a double-edged sword. An antibody’s large size creates ... tissue. Because antibodies are large and bivalent, primary and secondary antibodies cannot be incubated...
Showing: 21 - 40 of 224 results