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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 34 results for: buc

Showing: 21 - 34 of 34 results

  1. Luciferase Plasmid Collection

    Type
    Collection
    ...expressed if cells are infected with virus Christopher Buck 98580 pLX304 Luciferase-V5 blast Firefly CMV Lentiviral...

  2. Fluorescence Titering Assay

    Type
    Protocol
    ... pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste...

  4. General Transfection

    Type
    Protocol
    ... pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste...

  5. Protocol - Bacterial Transformation

    Type
    Protocol
    ...Stationary incubator at 37 °C Water bath at 42 °C Ice bucket filled with ice Microcentrifuge tubes Sterile spreading...

  6. Lentivirus Production

    Type
    Protocol
    ... pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste...

  7. Colony Formation Titering Assay

    Type
    Protocol
    ... pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste...

  10. Pouring LB Agar Plates

    Type
    Protocol
    ... agar. Autoclavable flasks Sterile pipettes Ice bucket to hold antibiotic Antibiotic at 1000 x concentration...

  11. AAV ddPCR Titration

    Type
    Protocol
    ...Mini Centrifuge, Thermo Scientific, 10199-452 Ice bucket 96-well freezer blocks (x 3) Reagents Molecular...

  12. AAV Production in HEK293 Cells

    Type
    Protocol
    ...channel pipette Hemocytometer or cell counter Ice bucket CO 2 incubator Pipet controller Hazardous waste...

  13. CRISPR Library Amplification

    Type
    Protocol
    ... and 10 mL Serological pipettes Ice slurry (Ice bucket with ice and water to create slurry) 100 mL LB ...

  14. Lentivirus ddPCR Titration

    Type
    Protocol
    ...multichannel pipette 20–200 µL multichannel pipette Ice bucket 96-well freezer blocks Vortex, VWR, 10153-688 Mini...
Showing: 21 - 34 of 34 results