We narrowed to 63 results for: cDNA
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TypeBlog Post... created for a variety of applications including cDNA expression, shRNA-mediated knockdown, Tet and Cre-regulated...
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MXS Chaining
TypeBlog Post...of these species and can often be used to clone cDNAs from them. These enzymes might not, however, be ... -
Plasmids 101: Gateway Cloning
TypeBlog Post...types of DNA fragments may be cloned: PCR fragments, cDNA or Genomic DNA and is available for all kind of ... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post...types of pooled libraries here at Addgene, including cDNA, barcoding, screening, and gRNA libraries. Most ... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...fragments from the organism of interest, or a defined cDNA library (e.g. the entire proteome of a particular... -
Open Enzyme Collection
TypeCollection...pOpen-PstIR PstI 165528 pOpen-SfiIR (cDNA) SfiI (used to create cDNA libraries) 165529 pOpen-DpnIR DpnI ... -
Cancer Research Plasmids and Resources
TypeCollection...vectors from the Sabatini & Wood Labs containing cDNAs for studying oncogenic signaling pathways. ClonTracer... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...proteins are made by connecting its DNA code to the cDNA encoding for another protein. Effectively, it equips... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog Post...reactions also contain reverse transcriptase to produce cDNA. Isothermal amplification is done at one constant... -
Plasmid Collections
TypeCollection... view all pooled libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular... -
Guide to Using Pooled Libraries
TypeGuide...to certain gene sets. In cDNA libraries, each plasmid contains a unique cDNA. In shRNA or gRNA libraries...PCR-amplified and sequenced using NGS to identify their cDNA or target gene. Positive selection screens are generally... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post...for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin, the gene mutated in muscular dystrophy... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post..., solubility and purification tags, connectors, cDNAs, and more to find the perfect combination. The EcoFlex... -
Bacterial Expression Systems
TypeCollection...localization of your gene of interest once its appropriate cDNA has been fused to the MS2 binding sites within this... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post... A.B. Lassar, Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell, 1987. 51... -
Gamma-Retroviral Vector Guide
TypeGuide...RNA is reverse transcribed into the provirus, the cDNA that will be integrated into a host genome. This...leading to disease. Provirus The genetic material (cDNA for retroviruses) that will be integrated into a... -
Delivery Methods for Generating iPSCs
TypeBlog Post... They contain only a eukaryotic promoter and the cDNA(s) to be expressed and they don’t integrate or replicate... -
Tetracycline Inducible Expression
TypeCollection...using fluorescence; use with pGFPns-reporter for cDNA target rtTA On Gu 60495 pSBtet-GP Sleeping Beauty... -
Neurodegeneration Research Collection
TypeCollection...(Link opens in a new window) such as huntingtin cDNAs, antibodies, and cell lines. Neurodegeneration Method...