We narrowed to 61 results for: cDNA

1. Your Lentiviral Plasmid FAQs Answered

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   ... created for a variety of applications including cDNA expression, shRNA-mediated knockdown, Tet and Cre-regulated...

2. Viral Vectors 101: Preparing Pooled Libraries

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   ...types of pooled libraries here at Addgene, including cDNA, barcoding, screening, and gRNA libraries. Most ...

3. MXS Chaining

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   ...of these species and can often be used to clone cDNAs from them. These enzymes might not, however, be ...

4. Plasmids 101: Gateway Cloning

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   ...types of DNA fragments may be cloned: PCR fragments, cDNA or Genomic DNA and is available for all kind of ...

5. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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   ...of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication...

6. Tips for Screening with Yeast Two Hybrid Systems

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   ...fragments from the organism of interest, or a defined cDNA library (e.g. the entire proteome of a particular...

7. Open Enzyme Collection

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   ...pOpen-PstIR PstI 165528 pOpen-SfiIR (cDNA) SfiI (used to create cDNA libraries) 165529 pOpen-DpnIR DpnI ...

8. Cancer Research Plasmids and Resources

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   ...vectors from the Sabatini & Wood Labs containing cDNAs for studying oncogenic signaling pathways. ClonTracer...

9. Plasmid Collections

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   ... view all pooled libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular...

10. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    ...reactions also contain reverse transcriptase to produce cDNA. Isothermal amplification is done at one constant...

11. Fluorescent Proteins 101: When GFP lets you down

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    ...proteins are made by connecting its DNA code to the cDNA encoding for another protein. Effectively, it equips...

12. Guide to Using Pooled Libraries

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    ...to certain gene sets. In cDNA libraries, each plasmid contains a unique cDNA. In shRNA or gRNA libraries...PCR-amplified and sequenced using NGS to identify their cDNA or target gene. Positive selection screens are generally...

13. Plasmids 101: Using Transposons in the Lab

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    ...for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin, the gene mutated in muscular dystrophy...

14. Plasmids 101: Modular Cloning Applications and Kits

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    ..., solubility and purification tags, connectors, cDNAs, and more to find the perfect combination. The EcoFlex...

15. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    ... A.B. Lassar, Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell, 1987. 51...

16. Gamma-Retroviral Vector Guide

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    Guide

    ...RNA is reverse transcribed into the provirus, the cDNA that will be integrated into a host genome. This...leading to disease. Provirus The genetic material (cDNA for retroviruses) that will be integrated into a...

17. Delivery Methods for Generating iPSCs

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    ... They contain only a eukaryotic promoter and the cDNA(s) to be expressed and they don’t integrate or replicate...

18. Neurodegeneration Research Collection

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    ...(Link opens in a new window) such as huntingtin cDNAs, antibodies, and cell lines. Neurodegeneration Method...

19. CRISPR Plasmids - Tagging

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    ...: Dalvai et al. Cell Rep. 2015 . Alternatively, cDNAs can be cloned directly into this vector and targeted...

20. 28 Hot Plasmid Technologies from 2015

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    ...constitutive (CMV, SV40, SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30...CreERT2 gene deletion, (3) simultaneous expression of cDNAs and shRNAs with a reporter gene, or (4) multiple...luciferase-fluorophore pH fusion reporters, pRSETb-pHlash and pcDNA3.1+-pHlash, that use Bioluminescence Resonance Energy...