We narrowed to 202 results for: cycs

...reverse tetracycline-controlled transactivator) is normally inactive and cannot bind the tetracycline response... inducible promoters. The positive inducible tetracycline ON (Tet-On) system, a versatile tool developed...response elements (TRE) in a promoter. Tetracycline and its derivatives serve as inducing agents to allow...Addgene.org: Inducible Promoters for Bacteria Tetracycline (Tet) Inducible Systems at Addgene Cre-lox Guide...

...by titration with different amounts of tetracycline. Tetracycline regulation is reversible (when the drug...with more elements of control. Tet promoters Tetracycline-dependent promoters are engineered by placing...constitutively silenced proteins. Addition of tetracycline or one of its analogs will induce these transcriptional...transcriptional level.     Fig. 2 Tetracycline on/off promoters and modes of action Check out...

...two PiggyBac integratable cassettes, both with doxycycline inducible promoters (Fig. 2a). The first cassette...contrast. In all the cell lines we tested, 1 µg/mL doxycycline was sufficient to saturate expression. When ...Next, perform a short (12-hour) induction with doxycycline prior to FACS, gating for the top quartile (or...producing cells. After FACS, return the cells to doxycycline-free media and expand the cells. Use a subset...engineered cells via regular intraperitoneal doxycycline injections to enable their tracking with ultrasound...

...Xerox for recycle and reuse, reducing the need for the production of new plastic.  Recycling and reuse...areas provide multiple streams for composting and recycling in the event the object in question cannot be ... We participate in many styrofoam and plastic recycling programs in the lab as well. For a more in-depth...

...our consumption beyond recycling.     Choosing more sustainable products Recycling is great for rerouting... post focused on streaming some lab waste for recycling- a process that’s integrated into waste management...Additional resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene...

...workers into three cycles: Doers, Managers and Leaders. I like her use of the term “cycles” instead of levels...roles. At any stage in our careers we may be in a cycle of doing, managing or leading. All three roles are...need to do to be successful in a specific role or cycle. For example, if you try to lead when you are expected...

...permeability Bactericidal 10-100 ug/mL Tetracycline tetracyclin Binds 30S ribosomal subunit; inhibits...aliquots at -20oC and avoiding repeated freeze/thaw cycles will keep most antibiotics viable for at least ...in foil if a light-sensitive antibiotic like Tetracycline is used. Keep in mind that some E. coli strains...

...SCM-1, SCM-1a, SCM1, SCYC1 XCL2 chemokine (C motif) ligand 2 SCM-1b, SCM1B, SCYC2 XCR1 chemokine (C motif...CDC42 cell division cycle 42 (GTP binding protein, 25kDa) CDC42Hs, G25K CDK4 cyclin-dependent kinase 4 ... formation of a high avidity BCR through repeat cycles of expansion. Chemokines Chemokines, or chemotactic... formation of a high avidity BCR through repeat cycles of expansion. Symbol Name Synonyms AKT1 v-akt murine...HHT, HHT2, ORW2, SKR3, TSR-I ADCYAP1R1 adenylate cyclase activating polypeptide 1 (pituitary) receptor type...BN, GRP-10, preproGRP, proGRP GUCA2A guanylate cyclase activator 2A (guanylin) GUANYLIN, GUCA2, STARA ...

...2. Viral stocks may be sensitive to freeze thaw cycles. Depending on the researcher and the type of virus... of titer losses from 5% to 50% per freeze thaw cycle (Krajden et al., 1999;  Ugai et al., 2002). If the...However, polybrene is highly sensitive to freeze-thaw cycles and should be stored in single-use aliquots. Never...Krajden, Mel, et al. "Effect of multiple freeze-thaw cycles on hepatitis B virus DNA and hepatitis C virus ...

... vector. In the absence of tetracycline or a derivative such as Doxycycline (Dox), the Tet-controlled ...needs to be expressed a certain point in the cell cycle, a transfer vector with an inducible promoter (see...used inducible lentiviral vector system is the tetracycline (Tet)-regulated system. You can choose either...

...complete each cycle, your best-performing mutant is usually the starting point for the next cycle. ...methods is that they all follow the same general cyclical process (Figure 2). The differences typically ...evolution experiments, you’ll typically go through this cycle many times, until you achieve whatever goal you’...

...other posts on lab sustainability, check out my Recycling and Innovations posts. Making small changes in...sustainability project. This could be: Setting up a recycling program at your institute. Set your -80 °C freezer...Additional resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene...

...editing The best studied Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal...genome editing system that could be regulated by doxycycline. Now, they’ve developed another AAV toolkit -...system is regulated not only temporally with doxycycline, but it can also be regulated spatially with ...

...creating less waste.  Most communities seem to have recycling programs and it seems to me that most Addgenies...fewer people use plastic bags.  We also try to recycle our discards according to the Cambridge, Massachusetts...chipped or imperfect equipment.  We can’t safely recycle these materials either.  Test tubes aren’t cheap...

...repeated cycles will favor accumulation of the latter products. As noted above, a single cycle of cleavage... Unlike HDR, NHEJ is active throughout the cell cycle and has a higher capacity for repair, as there is...

...these components as part of its natural viral life-cycle, and researchers have adapted the components for...recombination” (although later referred to as the “cyclization recombinase”), is a 38 kDa protein responsible...the Cre inducible (such as with tamoxifen or doxycycline), the Cre recombinase can be expressed only in...

...added to the mastermix that are read after each cycle. The raw data is then normalized to a reference ...dependent on number of samples rather than number of cycles.  Choosing a method  As with all experiments, a...analysis of copy number variants based on real-time LightCycler PCR. Curr Protoc Hum Genet. 2014;80:7.21.1-7.21...

...there are many places where the PhD application cycle becomes much more complex. “How do I reach out to...respond to your email. During my PhD application cycle, I reached out to around twelve professors and heard...like that! For instance, during my application cycle, two schools on opposite sides of the country had...

...exist. The life cycle of AdVs is dependent on modulation of the host cell’s cell cycle; inhibition of ...., 2003).        Figure 1: The adenovirus life cycle. Image reused from Kremer and Nemerow 2015 under...

...stimulate quiescence, the resting phase of the cell cycle; quiescent cells do not take up nucleic acid or ...lentiviral transduction. The effect of freeze-thaw cycles on lentiviral stability depends on a variety of...produced at pH 6 are resistant to multiple freeze thaw cycles. Consequently, it is best to avoid freeze-thawing...