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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 15 results for: cycs

Showing: 1 - 15 of 15 results

  1. AAV ddPCR Titration

    Type
    Protocol
    ...sealed, proceed to thermocycling. Thermal Cycling Run the following PCR parameters. Cycling Step Temperature...Droplet Digital PCR System, Bio-Rad, DX200 Thermal Cycler, Bio-Rad, T100 PCR Plate Sealer, Bio-Rad, PX1 1...Temperature (°C) Time (min) Ramp Rate (°C/sec) # Cycles Denaturation 95 10 2 1 Denaturation 95 0.5 2 50 Annealing...

  2. Lentivirus ddPCR Titration

    Type
    Protocol
    ...sealed, proceed to thermocycling. Thermal Cycling Run the following PCR parameters: Cycling Step Temperature...Droplet digital PCR System, Bio-Rad, DX200 Thermal Cycler, Bio-Rad, T100 PCR Plate Sealer, Bio-Rad, PX1 1...Temperature (°C) Time (min) Ramp Rate (°C/sec) # Cycles Enzyme Activation 95 10 2 1 Denaturation 94 0.5 2 40 ...

  3. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ... heating and cooling (denaturing and annealing) cycle. The process became automated with the discovery...polymerase can withstand many heating and cooling cycles, which would denature DNA polymerases from other...a few nanograms of template DNA through several cycles of denaturation, annealing, and synthesis. After... 2 minutes at 72°C. Repeat steps 8-10 for 25-30 cycles. Final Extension for 5 minutes at 72°C. Run 2 μL...

  4. Kit Free RNA Extraction

    Type
    Protocol
    ...avoid multiple freeze-thaw cycles. Pro-Tip To avoid multiple freeze-thaw cycles of your entire RNA sample...

  5. Fluorescence Titering Assay

    Type
    Protocol
    ...lentiviral supernatants to multiple freeze-thaw cycles. Procedure Seed 75,000 cells into each well of ...and debris. Lentiviral titer can decrease during cycles of freeze-thaw. If you are freezing and aliquoting...

  6. Colony Formation Titering Assay

    Type
    Protocol
    ...supernatants be subjected to multiple freeze-thaw cycles. This protocol outlines the seeding of the cells...and debris. Lentiviral titer can decrease during cycles of freeze-thaw. If you are freezing and aliquoting...

  7. Pouring LB Agar Plates

    Type
    Protocol
    .../mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg/mL Notes: Unless otherwise indicated...from the autoclave. Pro-Tip Once your autoclave cycle is complete, we recommend opening the door to the...

  8. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...or -80°C for long-term storage. TIP: Freeze/thaw cycles decrease the efficiency of the virus, so Addgene...into smaller tubes to prevent multiple freeze/thaw cycles. Back to Top F. Infecting Target Cells Lentiviral...

  12. Ligation Independent Cloning

    Type
    Protocol
    ...product is then repaired during the replication cycle. Empty vectors for LIC typically employ type II ...
Showing: 1 - 15 of 15 results