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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 39 results for: ena/1000

Showing: 21 - 39 of 39 results

  1. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...you want to have 100x-1000x coverage of your library: in other words, 100-1000x more reads than number...proper use requires careful amplification and maintenance of good representation. Addgene wishes you luck...

  2. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...Potential. PLoS Genet 5:e1000683 . https://doi.org/10.1371/journal.pgen.1000683 Bétermier M, Bertrand P...journal.pgen.1004086 Ghezraoui H, Piganeau M, Renouf B, Renaud J-B, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson...

  3. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...Research in Neurobiology, 4, 100086. https://doi.org/10.1016/j.crneur.2023.100086. Challis, R. C., Ravindra...antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing (Chen et al., 2023). AAV-BI30, developed...S., Meltzer, J. C., Buzhdygan, T., Cheng, M. J., Sena-Esteves, M., Bennett, R. E., Sullivan, T. P., Razmpour...by engineering capsid–receptor interactions that enable crossing of the blood–brain barrier. PLoS Biology...carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral...Directed evolution of a family of AAV capsid variants enabling potent muscle-directed gene delivery across species...

  4. Antibody Guide

    Type
    Guide
    ...sonication to break DNA up into fragments of 300-1000 bps in length. Note: This sonication process may...identity to be easily confirmed via sequencing, and enables the variable region and constant regions of the...given antigen. A mature B cell can clone itself to enable higher production of a single antibody, but each...blot Western blots use antibodies to identify denatured proteins separated by size on an SDS-PAGE gel....additional processing steps, such as sonication. Denature proteins, using heat and/or chemicals, and run...primary antibody must be able to recognize the denatured configuration of the protein. For western blots...

  5. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator...generating cell lines using lentiviral vectors enables long-term protein expression studies. Moreover,...resistance (e.g., puromycin, blasticidin), which enables selection of a stable cell culture after transduction...Performing antibiotic selection on transduced cells enables elimination of untransduced cells, resulting in...

  6. Bacterial Expression Systems

    Type
    Collection
    ...Consortium Modular Assembly Kits (Addgene #1000000005, 1000000010) contain plasmids and an engineered strain...localization to the periplasm. The pCri System (Addgene #1000000058) plasmids can be utilized for heterologous cytoplasmic...pastoris . The EcoFlex MoClo Toolkit (Addgene #1000000080) features a library of promoters and purification... cpFRET - FRET-based Biosensors Kit (Addgene #1000000021) is a vector library for the generation of FRET...ratiometric measurements. The MoBiFc Toolkit (Addgene #1000000222) is a collection of Golden Gate compatible vectors... repression. The BIOFAB plasmid set (Addgene #1000000037) contains specific bacterial promoters that vary... . The Marionette Sensor Collection (Addgene #1000000137) allows you to independently control the expression...

  7. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...Kamenyeva et al, 2015. Cellular trafficking studies enabled by fluorescent proteins The optimization of GFP...live-cell imaging (intravital imaging) studies it’s enabled are revealing the cellular behaviors that mediate...antigen presenting cells, but two-photon microscopy enabled scientists to see how chaotic and random the scanning...of fluorescent pathogens and fluorescent cells enables scientists to monitor cell interactions in diverse...proteins are excited by 750 nm to 760 nm laser light, enabling dual color imaging studies with blue or cyan proteins...photoactivable or photoconvertible proteins, as it enables tracking throughout the lifetime of the cell.     ...2009): 6379-6393. PubMed PMID: 19414791. 7. John, Beena, et al. "Dynamic imaging of CD8+ T cells and dendritic...

  8. Pipetting Protocol

    Type
    Protocol
    ...middle is hundreds, and the third is tens. Therefore, 1000 µL would read as 100 from top to bottom (as shown...molecular biology. You’ll use a pipette in many scenarios to accurately dispense small amounts of liquid...Anatomy of a Pipette This front view photo of a P1000 pipette shows the plunger at the top, which is used...tip off of the pipette. This side view photo of a P1000 pipette shows the volume adjustment ring right below...come in standard sizes: P2, P10, P20, P200, and P1000. The table shows the pipette with the appropriate...This image shows the P2, P10, P20, P100, P200, and P1000 as shown from left to right. Pipette Dispense Volume... 2 to 20 µL P100 10 to 100 µL P200 20 to 200 µL P1000 100 to 1,000 µL Each pipette should be used with...

  10. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ... of the biomedical research sector, and what a tenable workforce structure may look like. The panel discussion...Research: a perspective from junior scientists." F1000Research 3 (2014). Pubmed PMID: 25653845. Pubmed Central...

  11. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... variety of plasmids. Speed The Gateway system enables the generation of the expression construct in only...Drawbacks. Clon Transgen 4:138. doi:10.4172/2168-9849.1000138 2. Hartley JL. Use of the Gateway System for...

  12. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...Indeed, FRET was one of the first techniques which enabled the measurement of single molecule interactions...Bioessays. 2012 May; 34(5):341-50. doi: 10.1002/bies.201100098. Sekar RB, Periasamy A. Fluorescence resonance...

  14. CRISPR Guide

    Type
    Collection
    ...Gene and Genome Editing , 3–4 , 100013. DOI: 10.1016/j.ggedit.2022.100013 Zetsche, B., Gootenberg, J. S.... on the same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic...family proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal...purification methods are more straightforward and enable direct identification of molecules associated with...editor Fusion of a Cas protein to a deaminase that enables direct base conversion in RNA or DNA without a ...2016). Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian ..., Trevino, A. E., Joung, J., Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., ...

  16. TALEN Plasmids and Kits

    Type
    Collection
    ...Sangamo)-FokI-ELD-Destination Golden Gate TALEN 2.0 1000000030 Yamamoto Lab TALEN Construction and Evaluation... used for single-strand annealing assay, which enables the evaluation of TALEN activities in cultured ...-VP64 Golden Gate TALEN 2.0 47874 pTALYM3 Maria-Elena Torres-Padilla These plasmids were designed for ...

  17. Lentivirus ddPCR Titration

    Type
    Protocol
    ...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 2–50 µL multichannel pipette...°C/sec) # Cycles Enzyme Activation 95 10 2 1 Denaturation 94 0.5 2 40 Annealing/Extension 60 1 2 40 Enzyme...

  18. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... Alzheimer's Zena Werb 19138 mADAM10 deltaMP in pQCXIP cFLAG ADAM10 Flag Alzheimer's Zena Werb 19228 pBS...ERBB4 ERBB4 CMV ALS Yardena Samuels 29528 pcDNA3.1-ERBB4 (E317K) ERBB4 CMV ALS Yardena Samuels 29529 pcDNA3.1...E452K) ERBB4 CMV ALS Yardena Samuels 29530 pcDNA3.1-ERBB4 (E542K) ERBB4 CMV ALS Yardena Samuels 29531 pcDNA3.1...R544W) ERBB4 CMV ALS Yardena Samuels 29532 pcDNA3.1-ERBB4 (E563K) ERBB4 CMV ALS Yardena Samuels 29533 pcDNA3.1...dead) ERBB4 CMV ALS Yardena Samuels 29534 pcDNA3.1-ERBB4 (E836K) ERBB4 CMV ALS Yardena Samuels 29535 pcDNA3.1...(E872K) ERBB4 CMV ALS Yardena Samuels 29536 pCDF1-ERBB4 WT ERBB4 CMV ALS Yardena Samuels 29537 pCDF1-ERBB4...E317K) ERBB4 CMV ALS Yardena Samuels 29538 pCDF1-ERBB4 (E452K) ERBB4 CMV ALS Yardena Samuels 29539 pCDF1...

  19. CRISPR Guide

    Type
    Guide
    ...Gene and Genome Editing , 3–4 , 100013. DOI: 10.1016/j.ggedit.2022.100013 Zetsche, B., Gootenberg, J. S.... on the same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic...family proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal...purification methods are more straightforward and enable direct identification of molecules associated with...editor Fusion of a Cas protein to a deaminase that enables direct base conversion in RNA or DNA without a ...2016). Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian ..., Trevino, A. E., Joung, J., Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., ...
Showing: 21 - 39 of 39 results