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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 142 results for: endog

Showing: 21 - 40 of 142 results

  1. Promega Plasmid Collection

    Type
    Collection
    ...luciferase that enables highly sensitive detection at endogenous expression levels. NanoLuc fusions are useful...applications where you want minimal disruption of endogenous protein expression and interactions, such as ...disturbing the virus of interest and monitoring endogenous protein dynamics in highly scalable assays. HaloTag...dynamic range can detect low-abundance proteins at endogenous levels in lysates or live-cells, thus preserving...

  4. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...Biology Find the AsCas12f1 plasmids   Selective endogenous encapsidation for cellular delivery of RNAs By...in the capsid, in an approach called Selective Endogenous eNcapsidation for cellular Delivery (SEND). They...plasmids    Figure 1: SEND combines an endogenous Gag homolog, cargo RNA, and fusogen in a modular...

  5. Antibodies 101: Conjugation

    Type
    Blog Post
    ...conjugation. 1) Endogenous lysine residues, which can be found throughout the antibody. 2) Endogenous cysteines...the disulfide bonds between antibody chains. 3) Endogenous carbohydrates added to antibodies post-translationally...

  6. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ... Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally, dimeric Dronpa is... a target for MEK1 phosphorylation, much like endogenous ERK, and its localization serves as an indicator.... Zhou et al studied the negative feedback on endogenous MEK1/2 phosphorylation by creating a short pulse...

  7. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...cell which will recombine the template with the endogenous gene. Here, we'll describe the techniques and...Cre recombinase. If GFP is under control of an endogenous promoter, you can use expression GFP to track...responds. You can also use this method to tag an endogenous protein with GFP, as seen in blue flame plasmid...

  8. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ... rely on exogenous enzymes and those that use endogenous homologous recombination. Modifying bacterial...and homologous recombination As an alternative, endogenous homologous recombination enzymes can also accomplish... To allow manipulation of bacteria without an endogenous rhamnose regulon, we inserted a copy of rhaS ...

  9. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...Piwi-interacting RNA (piRNA) miRNA and piRNA can be found endogenously in many cell types, while shRNA and siRNA are... viral vectors or plasmids, shRNAs hijack the endogenous miRNA machinery for processing (Figure 1). The...structures to create a mature siRNA duplex. Fun fact! Endogenous miRNA production starts with transcription of...

  11. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Plasmids for Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging...plasmids can be used to create cell lines with endogenously-tagged cellular structures. Addgene ID Plasmid...

  12. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...encoded, photocaged Cas9 system could silence endogenous gene expression - demonstrating light-induced...2019) LADL: light-activated dynamic looping for endogenous gene expression control. Nat Methods 16:633–639...light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol 11:198–200 . https...

  13. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...transgenes and the up-regulation of corresponding endogenous pluripotency genes. Retroviral vectors are prone... and promoter demethylation and expression of endogenous pluripotency genes (i.e. Oct4) are other measures...Virus-Based Vector Using a Nonstructural C Protein and Endogenous MicroRNAs." Plos One 11, no. 10 (2016). PubMed...

  14. CRISPR Guide

    Type
    Collection
    ...Several labs have also controlled Acr activity with endogenous cell-type specific miRNAs to restrict CRISPR ...Validate genetic modification ). Browse Plasmids: Endogenous Tagging CRISPR Base Editing To overcome low HDR..., and the break can be repaired by the cell’s endogenous repair mechanisms. Figure 14: Overview of editing...repression gRNA G uide RNA , a synthetic fusion of the endogenous bacterial crRNA and tracrRNA that provides both...Jaenisch, R. (2013). Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional...Adamson, B. (2024). Improving prime editing with an endogenous small RNA-binding protein. Nature . 628 (8008...

  15. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...invader ( Figure 1 ). Figure 1: An overview of the endogenous Type II bacterial CRISPR system. Within the bacterial...allows us to create a variety of modifications in endogenous loci with unprecedented speed and specificity... Tag : Multiple methods make it easier to tag endogenous loci with epitope tags or fluorescent markers...

  16. Let There Be LITE Plasmids

    Type
    Blog Post
    ...it as “a novel mode of optogenetic control of endogenous cellular processes." The LITE system can now ...Konermann et al. "Optical control of mammalian endogenous transcription and epigenetic states." Nature....

  17. Tetracycline Inducible Expression

    Type
    Collection
    .... To avoid secondary effects from repurposing endogenous systems that respond to natural stimuli like ...al., 2011), engineered to eliminate binding of endogenous mammalian transcription factors to further minimize...their sequence and eliminating interactions with endogenous factors. Experimental Considerations Expression...

  18. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...coelenterazine, followed by the addition of the endogenous ligand or drug. In the inactive state, the luminescent...different GPCRs. This collection can be used to study endogenous ligands, the pathways they activate, as well ...

  19. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...GFP. If there’s GFP expression, presumably the endogenous target site will be cut and repaired by NHEJ ...exhibited a 2-4 fold increase in the number of endogenous indel edits when compared to unsorted cells (...
Showing: 21 - 40 of 142 results