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We narrowed to 188 results for: hwa

Showing: 21 - 40 of 188 results

  1. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...dissect a complex signaling pathway, and to identify what part of a pathway to inhibit to block the cellular...are causal for a phenotype of interest. Selected pathways  I attended a great talk when I was a PhD student...impression on me. The slide showed a complex signaling pathway with many receptors, signaling molecules, transcription... to show exactly which components of the known pathway that were essential for the activity of different...targeting all potential components of a signaling pathway. For example, one could study the cellular response...screen, known components of the cytokine signaling pathway could be targeted. Cells failing to upregulate ...

  2. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ...corresponding to known cellular signaling pathways as part of our Genetic Pathway Reference Set. These Gateway-compatible...cDNA clones mapping to well-annotated cellular pathways). It wasn’t clear whether gene-agnostic bioassays...The pan cancer mutant collection and the genetic pathway reference collections are described in this paper...PubMed Central PMCID: PMC5386591.  The genetic pathway reference collection is described in this publication...

  3. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...achieved in vivo using a bacterial recombination pathway that is present even in common lab cloning strains...strains. This intrinsic bacterial recombination pathway, referred to as recA-independent recombination, ...functions as a bacterial DNA repair mechanism. The pathway is ubiquitous, with successful recombination reported...E. coli strains tested so far. The use of this pathway as a cloning tool was first reported over 25 years...et al., 2016) uses the bacterial recombination pathway to allow any cloning procedure to be performed ...

  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...ACS Synth Bio. 2015. PubMed PMID: 26479688. RAS Pathway Clone Collection 2.0 Ras proteins are small GTPases...GTPases involved in cell signaling pathways that control many cellular processes such as differentiation...Understanding how molecules in the Ras signaling pathway interact will allow scientists to develop better... has recently released a collection of 360 Ras pathway plasmids to the scientific community. This collection... from a crowd-sourced map of the RAS signaling pathway and includes 180 unique transcripts. Each gene ...as to examine the mechanisms behind autophagic pathways. Rosado, et al. Autophagy. 2008. PubMed PMID:...-throughput tagging projects can be performed. Schwartz, et al. Genetics. 2016. PubMed PMID: 26837755 ...

  5. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...resistance gal Mutation in galactose metabolism pathway Cells cannot grow on galactose only gyrA, gyrA96...taken up by cell mcrA, mcrBC Inactivation of pathway that cleaves methylated cytosine DNA Allows for...methylated) DNA mrr, Δ(mcrC-mrr) Inactivation of pathway that cleaves methylated adenine or cytosine DNA...growth ara disruption of arabinose metabolism pathway Inability to utilize arabinose as a carbon source...growth proAB mutation in proline biosynthesis pathway Requires exogenous proline source for growth ...

  6. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...biosensors can enable investigation of a signaling pathway or measurement of a biomolecule while largely preserving...calcium signaling in the hippocampal mossy fiber pathway. Neuron. 2014 Apr 16;82(2):413-29. Baljit Khakh...imaging. Front Mol Neurosci. 2013 Sep 24;6:26. John Garthwaite cGMP (cyclic GMP) FRET-based cGMP biosensor SponGee...Cell. 2020 Aug 13. pii: tpc.20.00241. Markus Schwarzländer NADPH BRET semi-synthetic biosensor measuring...pyruvate A highly responsive pyruvate sensor reveals pathway-regulatory role of the mitochondrial pyruvate carrier...Synaptophysin with tandem repeats of pHluorin Two pathways of synaptic vesicle retrieval revealed by single-vesicle...dynamics. Nature. 2010 Jul 8. 466(7303):263-6. Martin Schwartz Tension Talin tension sensors Talin tension sensor...

  7. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...used to recreate natural biosynthetic pathways. Biosynthetic pathways containing multiple enzymes could be...improved cell-free system to examine biosynthetic pathways A new tool for enriching ABE-targeted cells The...transcription/translation system to examine biosynthetic pathways  By Gabrielle Clouse Recently, cell-free transcription...

  8. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...effectiveness. Analyze Cell Signaling Pathways Known cellular signaling pathways can be investigated in a high-throughput...promoter linked to luciferase. Activation of the pathway will result in luciferase expression and more complex...treatments can be designed to further explore the pathway. Which luciferase and assay have you used? Share...

  9. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...senses, GPCRs play roles in initiating signaling pathways in inflammation, and neurotransmission. These ...important molecular switches in signal transduction pathways and consist of a trimer of three subunits: alpha...there, how can scientists decipher the signaling pathways from ligand to receptor to G-protein, and finally...Interested in tracking the downstream signaling pathways mediated by GPCRs? The Kruse Lab constructed plasmids...

  10. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...important to know that cells possess two major repair pathways – Non-Homologous End Joining (NHEJ) and Homology...Homology Directed Repair (HDR) – and how these pathways work, as this could be relevant when planning your...structure, and dictate the major events of the pathway: Broken ends are recognized by loading of the ...This is a simplified, streamlined version of this pathway and does not consider the missing or damaged nucleotides...

  11. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...AKT, mTor, and ERK pathways so the lab will be studying the effects on these pathways using biosensors ...Terry Hébert: How GNB1 mutations affect signaling pathways G protein-coupled receptors (GPCRs) detect molecules...GNB1 disorder by understanding affected signaling pathways and how they lead to neurological phenotypes observed...

  12. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ..., chemogenetic receptors hijack cell signaling pathways and can lead to such responses as decreased heart...2014). The system allows you to study signaling pathways and the impacts of neural circuits on behavior...intracellular interactions and still set off the same pathways, giving rise to the same cellular responses. The...depends on what G-protein and, consequently, what pathway the receptor acts through. The DREADD hM3D(Gq),...

  13. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...guide RNA and repair template of your choosing (Schwartz and Jorgensen, 2016). Using SapTrap, this CRISPR...mutations that was published in a microPublication (Schwartz and Jorgensen, 2018).  These are just some examples...PMID: 17986455. PubMed Central PMCID: PMC2238901. Schwartz, Matthew L., and Erik M. Jorgensen. "SapTrap, ...: 26837755. PubMed Central PMCID: PMC4905529.  Schwartz, M. L., & Jorgensen, E. M. "SapTrap vectors for...

  14. Plasmid Collections

    Type
    Collection
    ...Finger Consortium Pathways & ORFs Plasmids are grouped together as part of a pathway or ORF. MAPK Nuclear...Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology Pooled Libraries Pooled...Nuclear Receptors Find a Pathway or ORF Kit Disease Research Find deposited plasmids for specific disease...

  15. CRISPR Guide

    Type
    Collection
    ...general repair pathways: the efficient but error-prone non-homologous end joining (NHEJ) pathway and the less...occurring repair pathway and is the primary repair mechanism for DSBs. While this pathway is efficient in...but high-fidelity homology directed repair (HDR) pathway. HDR relies on copying DNA from a matching template...involved in NHEJ or upregulating those in the HDR pathway can also help. Additionally, constructs with Cas9...fused components that promote the intended repair pathway or inhibit unwanted ones and improve editing efficiency...However, they promote the base excision repair pathway, which fully removes the uridine from the DNA and...evolved to excise inosine to access the same repair pathway and convert A to any other base. In principle, ...

  16. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ... Nicolas Mathis received his PhD from Gerald Schwank's lab at the University of Zurich (Switzerland),...L., Solari, C., Balázs, Z., Krauthammer, M., & Schwank, G. (2023). Predicting prime editing efficiency...Böck, D., Steensel, B. van, Krauthammer, M., & Schwank, G. (2024). Machine learning prediction of prime...on Cas9-induced DNA double-strand break repair pathway balance. Molecular Cell, 81(10), 2216-2230.e10....

  17. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...then transported in vesicles via the secretory pathway to their subcellular or extracellular destination...organelle or involved in intracellular transport pathways, it is possible to map the internal organization...markers for major mammalian organelles and transport pathways. It is best, though not always possible, to use....  CureFFI.org. Cell Biology 04: The Secretory Pathway. 4. Dunn, Kenneth W., Malgorzata M. Kamocka, and...

  18. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's Jens Schwamborn 85847 pDONOR-SNCAe3-WT SNCA CAG Parkinson's Jens Schwamborn 85848 pDONOR-SNCAe3...Parkinson's Jens Schwamborn 86154 pDONOR-PARK6e5-I368N PINK1 CAG Parkinson's Jens Schwamborn 87085 pCMV4-ApoE2... pRS-45 TBP His, yTAND12 T7 Parkinson's Harald Schwalbe 40822 EGFP-alphasynuclein-WT SNCA GFP CMV Parkinson's...CMV-CRY2oligo-mcherry-ANXA11 ANXA11 mCherry CMV ALS Jennifer Lippincott-Schwartz 164204 CMV-CRY2oligo-mcherry-ANXA11(D40G) ANXA11...ANXA11 mCherry CMV ALS Jennifer Lippincott-Schwartz 164205 CMV-CRY2oligo-mcherry-ANXA11(R235Q) ANXA11 mCherry...mCherry CMV ALS Jennifer Lippincott-Schwartz 164206 CMV-CRY2oligo-mcherry-ANXA11(R346C) ANXA11 mCherry...mCherry CMV ALS Jennifer Lippincott-Schwartz 164207 CMV-CRY2oligo-mcherry-ANXA11(1-185aa) ANXA11 mCherry CMV...

  19. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...understanding of the Z pathway" is vague and "what proteins regulate the Z pathway?" is an open-ended list...consider "how do proteins X and Y regulate the Z pathway?" A well-defined outcome makes it easier for the...postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes of Health, was an Associate...

  20. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...pinhead mutant, which regulates a developmental pathway implicated in congenital heart defects and cancer...lineage tracing can help illuminate how signaling pathways—such as those regulated by one-eyed pinhead—contribute...also showed that in the absence of the signaling pathway regulated by one-eyed pinhead, incorrect proportions... zygotes into adults and explain how signaling pathways control this process. Additional zebrafish applications...
Showing: 21 - 40 of 188 results