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We narrowed to 194 results for: lenti

Showing: 21 - 40 of 194 results

  1. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase...firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro - Lentiviral vector expressing ...examples of each in Addgene's collection: HIV-based lentiviral particles Murine leukemia-based retroviral particles...Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus...neutralizing activity are listed below: pBOBI-FLuc - Lentiviral reporter vector expressing firefly luciferase...firefly luciferase. pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase...luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter vector expressing mCherry and firefly...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Mammalian lenti sgRNA(MS2)_zeo backbone - 3rd gen lenti sgRNA cloning backbone with MS2 loops at tetraloop... genome pLenti CMV GFP DEST - Lentiviral Gateway destination vector for gene expression pLenti CMV/TO ...pLKO.1 hygro - Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant of lentiCRISPR v2 that confers...Blasticidin Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by...Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector for mammalian genome editing... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...

  3. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...expression? Check out our Retro expression vector and Lenti expression vector. Turn it on for just a little ...mechanism to integrate your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic ...transiently induce expression? Check out Addgene's Lentiviral dCas9 SAM vector and Piggybac dCas9 SAM vector...promoters and modes of action Check out Addgene's Lentiviral Tet-on and AAVS1 targeted Tet-on transgene vector...Adapted from Nabet et al. Check out Addgene's Lentiviral dTAG cloning vector and SMASh degron tagging ...Addgene website Promoter Guide CRISPR Guide Lentiviral Guide  ...

  4. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ... have quite a bit in common with AAV, just like lenti and gamma-retroviruses have a lot in common with...function. Lentivirus/gamma-retrovirus Lentiviruses are a class of retrovirus (so all lentiviruses are retroviruses... but not all retroviruses are lentiviruses.) This means that lentivirus and gamma-retrovirus share a lot... cargo into the host genome. Lentiviral-specific components Lentiviruses have gone through their fair ...are many shared components across viruses (AAV, lentivirus, etc.) and their functions are more intuitive...the lab. We will discuss the gamma-retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV)...for gamma-retroviruses. While not required for lentiviral production, they can be helpful for certain applications...

  5. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...S. pyogenes Puro Zhang lenti sgRNA(MS2)_zeo backbone 61427 Mammalian/Lentiviral BsmBI none S. pyogenes...Yang pLCKO 73311 Mammalian/Lentiviral hU6 none S. pyogenes Puro Moffat lenti sgRNA(MS2)_puro backbone 73795...pyogenes URA3 Davis lenti sgRNA(MS2)_puro optimized backbone 73797 Mammalian/Lentiviral U6 none S. pyogenes...Mammalian/Lentiviral BbsI none S. pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI...Mammalian/Lentiviral H1-Tet none S. pyogenes Herold lentiGuide-Crimson 70683 Mammalian/Lentiviral hU6 none...50662 Mammalian/Lentiviral See paper none S. pyogenes Blast Sabatini and Lander lentiCRISPR v2 52961 Mammalian...Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe pL-CRISPR.EFS.tRFP 57819 Mammalian/Lentiviral BsmBI...

  6. General Transfection

    Type
    Protocol
    ...cells optimized for viral production (AAVpro or Lenti-X), but any HEK293T cell line should work. This ...checking for protein expression. Sample Data Legend: Lenti-X 293T cells were transfected using 1:1, 1:2, 1:...

  7. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...protocol on Lentivirus Production provides a general approach for producing lentivirus in Lenti-X 293T cells...production of lentivirus. Purification of this lentivirus results in the pooled library lentiviral prep. B)... for using pooled lentiviral libraries. A) Amplification, validation, and lentiviral generation of a pooled...Usage of a pooled library lentiviral prep for experiments. Pooled library lentiviral preps are generally aliquoted...Pooled Lentiviral CRISPR Libraries- A nice overview of what to consider when using pooled lentiviral CRISPR.... Most of the pooled libraries at Addgene are lentiviral pooled libraries, though other types exist.  ...plasmids requires amplification, verification, and lentiviral production steps before you can start transducing...

  8. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...McClure MO, Weiss RA (1997) Sensitization of rhabdo-, lenti-, and spumaviruses to human serum by galactosyl(...target cells which produce lentiviral particles.   Why pseudotype? Lentiviruses and retroviruses typically...related to lentivirus, many retroviral envelope proteins have been pseudotyped on lentiviral particles.... rabies glycoprotein into the lentiviral envelope allows the lentiviral vector to be transported retrograde... Quick Guide to All Things Lentivirus Get tips for titering your lentiviral preps Resources on Addgene.org...Addgene.org: Check out the lentivirus guide Find ready-to-use lentiviral preps at Addgene ...To deliver genes using lentiviral vectors, you need an envelope protein on the virus’s surface and a ...

  9. Luciferase Plasmid Collection

    Type
    Collection
    ...plasmid Attila Karsi 105621 Lenti-luciferase-P2A-Neo Firefly EF1α Lentiviral expression of firefly luciferase...fusions using Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2-Nanoluc-ccdB NanoLuc® Creation ...fusions using Gateway cloning. Lentiviral. Mikko Taipale 87077 pLenti6.2-Firefly-ccdB Firefly Creation ...transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase...luciferase Eric Campeau 21474 pLenti CMV V5-LUC Blast (w567-1) Firefly CMV Lentiviral expression of firefly luciferase...Firefly Ubc Lentiviral expression of firefly luciferase Linzhao Cheng 74444 pLenti.PGK.blast-Renilla_Luciferase...Marcel Bruchez 108542 pLenti-EF1a-Luciferase-IRES-Blast-WPRE Firefly EF1α Lentiviral expression of firefly...

  10. Lentiviral Vector Guide

    Type
    Guide
    ... Guides Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...Browse lentiviral plasmids available at Addgene. Lentivirus Versus Gamma-Retrovirus Lentiviruses and gamma-retroviruses... Things Lentivirus Lentiviral Vector Uses and Overview Overcoming the Challenges of Lentiviral Production...community for decades. Wild-type lentiviruses have been engineered into lentiviral vectors that can be safely...main difference between lentiviruses and gamma-retroviruses is that lentiviruses are capable of infecting...that lentiviruses can infect a greater variety of cell types than retroviruses. Both lentiviruses and ...

  11. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Bonifacino 173579 Lenti-sgAtm#1/Cre ATM CMV Ataxia telangiectasia Charles Swanton 173580 Lenti-sgAtm#2/Cre ...Venus CMV Lewy body dementia Tiago Outeiro 89643 Lenti-sgAtm/Cre ATM U6 Ataxia telangiectasia Monte Winslow...FusionRed CUP1 ALS Michihiko Kataoka 188460 pKDR-Lenti-CBh.6-SOD1 SOD1 CBh ALS Erik Dent 188572 Tet-off...LRRK2 His T5 Parkinson's Suzanne Pfeffer 208392 Lenti-sgRNA-Cre-GpNLuc-sgmATM ATM Ataxia telangiectasia...Cookson 13318 pLenti6-DEST PINK1-V5 G309D PINK1 V5 CMV Parkinson's Mark Cookson 13319 pLenti6-DEST PINK1-... Cookson 29412 pLenti6-DJ1-V5-E18N PARK7 V5 CMV Parkinson's Mark Cookson 29413 pLenti6-DJ1-V5-C106D PARK7...Cookson 29414 pLenti6-DJ1-V5-L166P PARK7 His, V5 CMV Parkinson's Mark Cookson 29415 pLenti6-DJ1-V5-E18D ...

  12. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...descriptions of the lentiviral elements and the 2nd and 3rd Generation Lentiviral Systems can be found...found in our Lentiviral guide. Popular packaging and lentiviral envelope plasmids are listed here. Please...pre-treated with a compound of interest. Lentiviral vectors Popular Lentiviral Transfer Vectors    Expressing your...3rd generation lentiviral vector backbone from the Shih lab. You can find more lentiviral expression vectors...ready-to-use lentiviral prep from the Addgene viral service. CRISPR technology lentiCRISPR v2 (Plasmid ... ~10-fold higher titer of lentiviral particles than the original lentiCRISPR v1. Special note from the...in the genome editing field. Lentiviral Plasmid Kit Multiple Lentiviral Expression System Kit (Kit #1000000060...

  13. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...of producing CRISPR lentivirus. As we’ve heard from scientists, producing lentivirus is not always an easy... can make lentivirus production frustrating - a researcher can complete an entire lentiviral production...titering, testing, and providing lentiviral preps of select lentiviral vectors from the repository for ... in lentiviral format. Finally, while we can’t currently produce custom gRNA-containing lentivirus, we... make new lentiviral preps on your own, fear not - anytime you order ready-to-use lentivirus from Addgene...you can use along with lentiviral packaging plasmids to create your own lentivirus stocks. Our research ...re very excited to provide ready-to-use CRISPR lentiviral preps to researchers across the globe. Why provide...

  14. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...pSpCas9(BB)-2A-GFP (PX458). lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the...promoter. It’s a 3rd generation lentiviral backbone that must be used with lentiCas9-Blast or with cell lines...the last 15 years - the majority of these being lentiviral or CRISPR tools. How many times have these plasmids...Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral packaging plasmid from the Didier Trono lab that...genes. It can be used with 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such...the top requested plasmid of 2015. What makes lentiCRISPR v2 great? It produces a ~10 fold higher titer...CRISPR pooled library screening experiments. Find lentiCRISPR v2.(If you’ve peeked through the rest of the ...

  15. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...does it work? LentiCRISPR v2 purpose As its name suggests, lentiCRISPR v2 is a lentiviral transfer vector...the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab...designed to have increased lentiviral titer as compared to previous lentiviral vectors. It can be used both...respectively, use lentiCRISPR v2 as the backbone for their many gRNAs. The high titer lentivirus production ... the Addgene Website Visit our Lentiviral Guide Pages Find Lentiviral Plasmids Find CRISPR Pooled Libraries...production afforded by lentiCRISPR v2 is key to its efficient use in screening experiments; large amounts of virus...pooled library screens. Availability and Use LentiCRISPR v2 is available from Addgene both as a single...

  16. Viral Production at Addgene

    Type
    Blog Post
    ...Browse our AAV vector collection. Lentivirus Production Lentiviral preparations ("-LV” catalog items)...Concentrated lentiviral preparations (“-LVC” catalog items) are generated from lentiviral preparations...being frozen. Specifically, lentiviral particles are collected from the lentiviral preparation by precipitation...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...of the transfer plasmid.   Lentivirus resources Browse lentiviral preps available from Addgene. After...through how we produce both our AAV preps and our lentiviral preps.   Viruses are generated using standard... Cell culture medium (OptiPro SFM) containing lentivirus is first cleared by low-speed centrifugation ...

  17. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ... of 2017 is the lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector... producing our ready-to-use lentivirus preps. You can find our lentivirus production protocol on our website...titering your lentivirus in another blog post. If you need a quick primer on how lentiviruses work, check...2015 and 2016 were CRISPR plasmids), but so are lentiviral vectors, many of which are used to deliver Cas9...promoter. Envelope proteins are a key component of lentiviruses. These proteins coat the virus and help determine...widely used and requested because it enables lentiviruses to infect many different types of cells. This...check out our lentivirus guide page. For more protocols and information, be sure to check out the Trono Lab...

  18. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ... screens rely on lentiviral systems, a fact supported by the wide array of lentiviral-based CRISPR libraries... while both AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost...vector delivery methods. Lentivirus has limited packaging options, with most lentiviral vectors being packaged...large packaging capacity and stable expression of lentiviral vectors make them an attractive option. However...made at high titers equal to those achieved with lentivirus but offer an improved safety profile, customization...expression in many mouse tissues. Safety AAV and lentivirus have different safety profiles that often affect...experiments. AAV can be safely handled at BSL-1, while lentivirus requires a BSL-2 setup. This requires a less ...

  19. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...use lentivirus to deliver a pooled population of gRNAs to Cas9 expressing cells. Pooled lentiviral CRISPR...generate lentivirus (B). Cas9-expressing cells or wild-type cells are treated with lentivirus containing... Quick Guide to All Things Lentivirus Get Tips on Titering Your Lentivirus Plasmids 101: NGS Quality Control...a heterogeneous population of gRNA-containing lentiviral transfer vectors, each targeting a specific gene...synthesized. Pooled gRNAs are then cloned into lentiviral transfer vectors to create the CRISPR library... concentration that is sufficient to generate lentivirus. Be sure to use next-generation sequencing to...amplification. If you've obtained a ready-to-use lentiviral preparation from Addgene, you can skip the steps...

  20. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ... cells a virus can infect. Note: While both lentiviruses and γ-retroviruses use the same gene products... well as the viral LTRs, differ. As a result, lentiviral and γ-retroviral packaging plasmids are not interchangeable...using 293T cells This method is very similar to lentiviral packaging methods. The three components described...How do engineered γ-retroviruses differ from lentiviruses? There are a few important differences between...between engineered γ-retroviruses and lentiviruses. First, these viruses were derived from different genomes...genomes (MoMLV and MSCV for γ-retrovirus; HIV for lentivirus). Additionally, γ-retroviruses can only transduce...during mitotic breakdown of the nuclear envelope. Lentiviruses can transduce both dividing and non-dividing...
Showing: 21 - 40 of 194 results