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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 45 results for: nls

Showing: 21 - 40 of 45 results

  2. AAV Molecular Tools

    Type
    Collection
    ...System Activity Serotype PI 61592 pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA CMV-driven, constitutive Expression...

  3. Chemogenetics AAV Preps

    Type
    Collection
    ...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 hM4D(Gi) - Inhibition NLS-dTomato none 1, 9, rg* Fishell 83897 pAAV-hDlx-GqDREADD-dTomato-Fishell...pAAV-hDlx-GqDREADD-dTomato-Fishell-4 hM3D(Gq) - Activation NLS-dTomato none 1, 9, rg* Fishell 50472 pAAV-GFAP-HA-rM3D...

  4. Caltech Systemic Capsids

    Type
    Collection
    ...pAAV-CAG-eYFP CAG EYFP Control Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH...pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2 Dlx mRuby2 Control Gradinaru 104055 pAAV-CAG-eYFP...dTomato/nlsdTomato Control Fishell 213936 pAAV_BiPVe4_dTomato_nlsdTomato BiPVe4 dTomato/nlsdTomato Control...Control AIBS , Ting 213814 pAAV_BiSSTe10_dTomato_nlsdTomato BiSSTe10 dTomato/nlsdTomato Control Fishell 213829...213829 pAAV_BiCHATe27_dTomato_nlsdTomato BiCHATe27 dTomato/nlsdTomato Control Fishell 213855 pAAV_BiVIPe4...BiVIPe4 dTomato/nlsdTomato Control Fishell 213914 pAAV_BiLAMP5e3_dTomato_nlsdTomato_nlsdTomato BiLAMP5e3 dTomato...Control Fishell 213940 pAAV_BiPVe3_dTomato_nlsdTomato BiPVe3 dTomato/nlsdTomato Control Fishell 213944 pAAV_BiSSTe4...

  5. Biosensor AAV Preps

    Type
    Collection
    ...AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f dTomato Cre dependent rg* Ting 51085 AAV-hSyn1-GCaMP6f-P2A-nls-dTomato..., Huebener , Rose 51082 AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato EF1a GCaMP6s dTomato Cre dependent 1 Ting...Cre dependent 5 Larsen 51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s dTomato Constitutive 1, rg* Ting...

  6. TALEN Plasmids and Kits

    Type
    Collection
    ...and 47389 include a Flag epitope tag and an SV40 NLS at the N terminus, a 152-residue deletion from the...acids after the repeat domain, and C-terminal SV40 NLS and HA tags. Plasmid 47389 also contains the VP64...

  9. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...PhoCl in between a protein and a tag, such as an NLS or NES, enables control over localization. Inserting...

  10. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...FP) Guide and the Michael Davidson FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression...Gateway yeast expression vector that adds a SV40 NLS Myr Membrane localization pWZL-Neo-Myr-Flag-DEST ...C-terminus of BioID2 3xHA-TurboID-NLS_pCDNA3 or 3xHA-miniTurbo-NLS_pCDNA3 - Expresses 3xHA-tagged TurboID...expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression pENTR4...

  11. Luciferase Plasmid Collection

    Type
    Collection
    ...firefly luciferase Javier Alcudia 113442 pcDNA3.1 NL NanoLuc® CMV Mammalian expression of Nanoluc with... Renilla luciferase Rudolf Jaenisch 113450 pLenti NL NanoLuc® Ubc Lentiviral expression of Nanoluc with...

  12. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ... promoter pAdx-CMV-YFP 73348 Express YFP with an NLS from the CMV promoter pAdx-CMV-dsRed 73349 Expresses...

  13. Lentiviral Prep Service

    Type
    Collection
    ...dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang Pooled Barcoding...

  14. Molecular Biology Reference

    Type
    Guide
    ...GLNDIFEAQKIEWHE Factor Xa IEGR or IDGR VSVG YTDIEMNRLGK SV40 NLS PKKKRKV or PKKKRKVG Protein C EDQVDPRLIDGK S Tag ...

  15. Lentivirus Plasmids

    Type
    Collection
    ...packaging construct encoding Tat. Reiser 17531 pCD/NL-BH*DDD 2nd Packaging Contains Gag/Pol, Tat, Rev. ...

  16. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...pyogenes Marraffini pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) 47868 Mammalian U6 yes, cut N. meningitidis..., methylation S. pyogenes Puro Zoldos pBLO1811_Cas9_noNLS_human 74490 Mammalian U6 yes, cut S. pyogenes...pyogenes mCherry Savage pBLO1808_arC9_2xNLS_human 74491 Mammalian U6 yes, cut S. pyogenes mCherry Savage pSG4K5...Bacteria J23119 none S. pyogenes Wang pBLO1811_arC9_noNLS_human 74494 Mammalian U6 yes, cut S. pyogenes...

  17. Optogenetics AAV Preps

    Type
    Collection
    ...FusionRed Cre dependent 5 Yizhar 109048 pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST CAG eGtACR1 (soma-targeted...

  18. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...GFP-polyQ23-NLS HTT GFP, SV40 NLS CMV Huntington's Lukas Kapitein 231892 GFP-polyQ74-NLS HTT GFP, SV40 NLS CMV...IDR-mCherry-Cry2-NLS TBP SFFV Parkinson's Denes Hnisz 145270 pHR-TBP(53Q)-IDR-mCherry-Cry2-NLS TBP Parkinson's...GPD ALS Aaron Gitler 29595 416Gal-SV40NLS-FUS-YFP FUS YFP, SV40 NLS GAL1 ALS Aaron Gitler 29596 426Gal-FUS... pEGFP-TDP-43 (NLSmut) TARDBP GFP CMV ALS Maxime Rousseaux 235490 pEGFP-TDP-43 (PY-NLS) TARDBP GFP CMV...SNCA CMV Parkinson's Hilal Lashuel 107543 pAAV-AICD-NLS-IRES-hrGFP APP CMV Alzheimer's Helene Marie 107544... Halo Parkinson's Michael Ward 178658 WT A1-LCD +NLS HNRNPA1 His T7 ALS Tanja Mittag 178659 A1-LCD-12F... pcDNA3.2 TDP-43 NLS1 YFP TARDBP YFP CMV ALS Aaron Gitler 84913 pcDNA3.2 TDP-43 NLS1, 5F-L YFP TARDBP ...

  19. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...Optogenetics Jonathan Ting New viral prep pAAV_BiSSTe10_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Optogenetics Gordon Fishell New viral prep pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Optogenetics Gordon Fishell New viral prep pAAV_BiVIPe4_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Gordon Fishell New viral prep pAAV_BiLAMP5e3_dTomato_nlsdTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell...Optogenetics Gordon Fishell New viral prep pAAV_BiPVe4_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Optogenetics Gordon Fishell New viral prep pAAV_BiPVe3_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Optogenetics Gordon Fishell New viral prep pAAV_BiSSTe4_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...

  20. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

    Type
    Blog Post
    ...fused to a bipartite nuclear localization signal (bNLS) and a nuclear export signal (NES), both which are...localizes to the nucleus, but following phosphorylation bNLS activity is suppressed and NES activity is enhanced...positions of the phosphorylatable residues and the bNLS and NES sequences, which have previously been characterized...Kudo et al. for more details on the design of the bNLS and NES sequences. Disadvantages of KTRs KTRs also...
Showing: 21 - 40 of 45 results