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  1. Finding Your Science Policy Path

    Type
    Blog Post
    ...there is often discussion of “big P” and “little p” policy.  “Big P” policy is what you might typically...scientific areas. Whether you’re interested in “big P” or “little P” policy, the possibilities are endless leading... government itself. You can work to influence Big P policy from a post in non-governmental organizations...formal lobbying, or strategy setting.  The “little p” policy is non-governmental work happening at large...

  2. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...., Nalin, R., Sailland, A., Auriol, P., Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence ...Jessica Welch in January 2017 and was updated by Emily P. Bentley in October 2024. References and Resources...Veseli, I., Moore, R. M., Kiefl, E., Moody, T., Rice, P. A., Yu, M. K., Mimee, M., Chang, E. B., Ruscheweyh...doi.org/10.1016/j.cell.2024.01.039 Garcillán-Barcia, M. P., Redondo-Salvo, S., & de la Cruz, F. (2023). Plasmid...

  3. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...Sands, J., Sahasrabudhe, A., Anikeeva, P., & Chandrakasan, A. P. (2020). Modular Optoelectronic System...EMBC44109.2020.9175600 Park, S., Loke, G., Fink, Y., & Anikeeva, P. (2019). Flexible fiber-based optoelectronics for ...Niroui, F., Fink, Y., Chandrakasan, A., … Anikeeva, P. (2023). Multifunctional microelectronic fibers enable...

  4. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...j.cell.2020.04.031 Zimmermann I, Egloff P, Hutter CAJ, Kuhn BT, Bräuer P, Newstead S, Dawson RJP, Geertsma ...s41596-020-0304-x Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist...Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA (2018) Synthetic...

  5. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    ... protein interactions. J Cell Biol, 2003. 163(2): p. 257-69. PubMed PMID: 14581454. PubMed Central PMCID...microdomains of live cells. Science, 2002. 296(5569): p. 913-6. PubMed PMID: 11988576. 3. Matz, M.V., et al...nonbioluminescent Anthozoa species. Nat Biotechnol, 1999. 17(10): p. 969-73. PubMed PMID: 10504696. 4. Ohashi, T., et ... unstructured proteins. Protein Sci, 2007. 16(7): p. 1429-38. PubMed PMID: 17586775. PubMed Central PMCID...under physiologic conditions. Traffic, 2012. 13(5): p. 643-9. PubMed PMID: 22289035. PubMed Central PMCID...

  6. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...s41565-023-01419-x Deverman, B. E., Pravdo, P. L., Simpson, B. P., Kumar, S. R., Chan, K. Y., Banerjee, A...Hordeaux, J., Yuan, Y., Clark, P. M., Wang, Q., Martino, R. A., Sims, J. J., Bell, P., Raymond, A., Stanford,... A. I., Dvoryanchikov, G., Makhoul, V., Tsoulfas, P., & Chaudhari, N. (2022). Selectively Imaging Cranial...Griffiths, J. A., Ichiki, T., Mazmanian, S. K., Osborne, P. B., Keast, J. R., Miller, C. T., Fox, A. S., Chiu... N. M., Sun, J., Chen, X., Lefevre, A., Singh, V. P., Arokiaraj, C. M., Shay, T. F., Vendemiatti, J., ... A., Wu, W.-L., Yang, B., Huber, N., Pasca, S. P., & Gradinaru, V. (2016). Cre-dependent selection yields...Gore, B. B., Szelenyi, E. R., Trader, C., Balaram, P., … Tasic, B. (2021). Enhancer viruses for combinatorial...

  7. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ....   References Bennardo N, Gunn A, Cheng A, Hasty P, Stark JM (2009) Limiting the Persistence of a Chromosome...10.1371/journal.pgen.1000683 Bétermier M, Bertrand P, Lopez BS (2014) Is Non-Homologous End-Joining Really...Waters CA, Strande NT, Pryor JM, Strom CN, Mieczkowski P, Burkhalter MD, Oh S, Qaqish BF, Moore DT, Hendrickson....1038/ncomms5286 Yan M-Y, Li S-S, Ding X-Y, Guo X-P, Jin Q, Sun Y-C (2020) A CRISPR-Assisted Nonhomologous...

  8. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...plasmids and plasmids in incompatibility groups N, P, Q, and W are known to have replicons with broad host...Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia stuartii, Pseudomonas spp....Paracoccous denitrificans, Pseudomonas fluorescens, P. putida, Rhizobium meliloti, R. leguminosarum by. .../10.1074/jbc.273.14.8447  Jain, A., & Srivastava, P. (2013). Broad host range plasmids. FEMS Microbiology...2.50021-0  Toh, W. K., Teo, Y. L., Tor, X. Y., Loh, P. C., & Wong, H. L. (2023). Development of constitutive...

  9. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...Kateriya, S., Adeishvili, N., Berthold, P., Ollig, D., Hegemann, P., & Bamberg, E. (2003). Channelrhodopsin...Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann, P., & Wiegert, J. S. (2021). BiPOLES...Berndt, A., Sohal, V. S., Deisseroth, K., & Hegemann, P. (2010). Ultrafast optogenetic control. Nature Neuroscience....1936192100 Tichy, A.-M., Gerrard, E. J., Sexton, P. M., & Janovjak, H. (2019). Light-activated chimeric...

  10. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...., Feliciano, P. R., Arif, A., Bohnuud, T., Fernandez, T. P., Gehrke, J. M., Grayson, P., Lee, K. D., ...updated by Jennifer Tsang in August 2020 and by Emily P. Bentley in February 2025. References and Resources...Grünewald, J., Zhou, R., Lareau, C. A., Garcia, S. P., Iyer, S., Miller, B. R., Langner, L. M., Hsu, J...., Levy, J. M., Tay, T., Newby, G. A., Maianti, J. P., Raguram, A., & Liu, D. R. (2018). Improving cytidine...McElroy, A. N., Pandey, S., Doman, J. L., Huang, T. P., Raguram, A., Banskota, S., Newby, G. A., Tolar, ...Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained genome targeting with near-...

  11. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...experiment! References Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable...Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan... https://doi.org/10.1038/nature14136 Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein...Zhang J, Tang C, Zhang X, Xu H, Shi L, Cheng L, Huang P, Yang H (2018) In vivo simultaneous transcriptional...

  12. Of Myc and Men

    Type
    Blog Post
    ...than the substance P peptide, but still quite small. However, unlike the substance P tag, they found that...involved a peptide from the neuropeptide substance P. This protein had a highly specific antibody to the... the tag could be quite small. However, substance P has an amide group added to its C-terminus post-translationally...

  13. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine M, Broucque F, Robin C, Hebben M,...Merten O-W, Blouin V, François A, Redon R, Moullier P, Léger A (2015) Advanced Characterization of DNA Molecules..., Penaud-Budloo M, Broucque F, Blouin V, Moullier P, Ayuso E, Vandenberghe LH (2017) AAV-ID: A Rapid and...

  14. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...en.wikipedia.org/wiki/File:Crispr.png Barrangou R, Horvath P. Science. 315, 2007 Jan; 1709–1712 (2007). CRISPRdb..., Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineu S, Mojica FJM, Wolf YI, Yakunin JvdO, Koonin... F. Science. 2013 Jan; 339 (6121): 819–23. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville...

  15. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...y Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S. E., Muller... C., Ingelsson, M., Chamberlain, J. S., Corey, D. P., Aryee, M. J., Joung, J. K., Breakefield, X. O., ...

  16. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...., Thomas, D., Stamnes, S., Holt, K., Sinn, P., May, A. P., & Paulk, N. K. (2020). Methods Matter: Standard... F., Le, T., Prado, J., Bahr-Davidson, J., Smith, P. H., Zhen, Z., Sommer, J. M., Pierce, G. F., & Qu,... https://doi.org/10.1016/j.ymthe.2005.02.021 Zuk, P., Sanchez, C. E., Kostick, K., Torgerson, L., Muñoz...

  17. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ... a lentiviral vector. Naldini L, Blömer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, Trono D. Science...Greenberg RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas P, June CH, Humeau LM, Rebello T. J Gene Med. 2013 Feb...Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer A, Cornetta K, Galacteros F, Beuzard Y, Gluckman...Gluckman E, Bushman F, Hacein-Bey-Abina S, Leboulch P. Nature. 2010 Sep 16;467(7313):318-22. PubMed PMID...

  18. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...and other important information. Tip: A lowercase "p" is often used as the first letter of a plasmid name...and simply denotes that the object is a ‘plasmid’. “p” is for plasmid. pXXXXX-XXXX Step 1: Backbone name...

  19. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...which identifies a p value cut off. This method entails arranging all calculated p values from smallest...this by establishing a significance threshold at a p value less than or equal to the alpha value divided...the cut off for significance by finding the largest p value where its rank divided by the total number of...multiplied by the alpha value is less than or equal to the p value. There are drawbacks to these tests as they ...

  20. Kit Free RNA Extraction

    Type
    Protocol
    ...adapted from (Link opens in a new window) Chomczynski P and Sacchi N, 2006 and (Link opens in a new window...
Showing: 21 - 40 of 230 results