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We narrowed to 111 results for: quo

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Showing: 21 - 40 of 111 results

  1. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...molecular biologists bought the pricey stuff in 50 ul aliquots in a kit with a huge amount of wasteful packaging...from a pottery supply house.  These large, cheap aliquots could be stored indefinitely at room temperature...

  2. Antibody Guide

    Type
    Guide
    ...long-term storage they should be stored at -20 °C in aliquots of at least 10 µL. Antibodies cannot tolerate ... some cases, it may be preferable to buy small aliquots of many different antibodies and do batch validation...

  4. Lab Automation at Addgene

    Type
    Blog Post
    ... any project in which you need to do a lot of aliquoting. Simply program the pipette with how much total... sample you need and the volume of the smaller aliquots you’d like to dispense and you’re on your way....

  5. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Mammalian Expression mTurquoise2 434 474 28 3.1 33.5 min Monomer (A206K) pmTurquoise2-C1 - Mammalian Expression...Expression pmTurquoise2-N1 - Mammalian Expression CyPet 435 477 18 5 Monomer pCEP4CyPet-MAMM - Mammalian...

  6. Hot Plasmids - August 2020

    Type
    Blog Post
    ...genetically encoded fluorescent markers tagged with mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list...service By Ina Ersing We regularly add new viral aliquots from our plasmid collection to provide ready-to-use...

  7. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...conditions, with pKa values below 4. Examples include mTurquoise2 (pKa = 3.1), tagRFP (pKa = 3.1) and mCherry (...pKa = 3.8). We have observed that mCherry (and mTurquoise2, not shown) remains fluorescent in acidic organelles...

  8. Important Considerations When Using AAVs

    Type
    Blog Post
    ...AAV titer. For long term storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze in liquid nitrogen...bath, and store at -80° C. Once thawed, the AAV aliquot should be used quickly. 3. Patience Is Bitter, ...

  9. Viral Production

    Type
    Collection
    ...adequate for in vivo studies. Preparations are then aliquoted and stored at -80 °C. Titer Titering is either...polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, and stored at -80 °C. Titer All titering...

  10. Bacterial Expression Systems

    Type
    Collection
    ...Miguel Valvano 47640 pCS-PesaRlux PesaR 3OC6HSL (Quorum sensing molecule) Escherichia coli Cynthia Collins...coli Remy Loris 47663 pAC-EsaR-I70V Plac 3OC6HSL (Quorum sensing molecule) Escherichia coli Cynthia Collins...

  11. Antibody Guide

    Type
    Collection
    ...long-term storage they should be stored at -20 °C in aliquots of at least 10 µL. Antibodies cannot tolerate ... some cases, it may be preferable to buy small aliquots of many different antibodies and do batch validation...

  15. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...without producing off-target mutations," Sha was quoted as saying when the news first broke. "Considering...
Showing: 21 - 40 of 111 results