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  2. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...bioluminescent properties observed in the crystal jellyfish Aequorea victoria (Figure 1) in molecular detail. On the...jellyfish’s photoorgans; the first, which he called aequorin, gives off a faint blue light when it binds calcium...

  3. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...molecular biologists bought the pricey stuff in 50 ul aliquots in a kit with a huge amount of wasteful packaging...from a pottery supply house.  These large, cheap aliquots could be stored indefinitely at room temperature...

  5. Lab Automation at Addgene

    Type
    Blog Post
    ... any project in which you need to do a lot of aliquoting. Simply program the pipette with how much total... sample you need and the volume of the smaller aliquots you’d like to dispense and you’re on your way....

  6. Bacterial Expression Systems

    Type
    Collection
    ...3OC6HSL (Quorum sensing molecule) Cynthia Collins Contains luciferase inducible by the quorum sensing ...

  7. Hot Plasmids - August 2020

    Type
    Blog Post
    ...genetically encoded fluorescent markers tagged with mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list...service By Ina Ersing We regularly add new viral aliquots from our plasmid collection to provide ready-to-use...

  8. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...conditions, with pKa values below 4. Examples include mTurquoise2 (pKa = 3.1), tagRFP (pKa = 3.1) and mCherry (...pKa = 3.8). We have observed that mCherry (and mTurquoise2, not shown) remains fluorescent in acidic organelles...

  9. Important Considerations When Using AAVs

    Type
    Blog Post
    ...AAV titer. For long term storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze in liquid nitrogen...bath, and store at -80° C. Once thawed, the AAV aliquot should be used quickly. 3. Patience Is Bitter, ...

  10. Antibody Guide

    Type
    Collection
    ...long-term storage they should be stored at -20 °C in aliquots of at least 10 µL. Antibodies cannot tolerate ... some cases, it may be preferable to buy small aliquots of many different antibodies and do batch validation...

  12. Synthetic Biology - Overview

    Type
    Collection
    ...Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard Endy Lab Logic Gates...

  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's, FTD Michael Davidson 55570 mTurquoise-MAPTau-N-10 MAPT mTurquoise CMV Parkinson's, FTD Michael Davidson...Parkinson's David Komander 110945 pmTurquoise-parkin PRKN mTurquoise2 CMV Parkinson's Pablo Rivera-Fuentes...

  14. Antibody Production

    Type
    Collection
    ...of antibodies and suggests preparing single-use aliquots before storing at -20 °C. Each antibody catalog...

  17. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...without producing off-target mutations," Sha was quoted as saying when the news first broke. "Considering...
Showing: 21 - 40 of 110 results