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Showing: 21 - 40 of 72 results

  1. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...metagenomically discovered marine anion-conducting channelrhodopsin used as a sensitive fluorescent voltage indicator...Hyperpolarizing chloride channel SwiChr++ - A channelrhodopsin converted into a chloride-conducting anion... step-function artificial anion conducting channelrhodopsin (aACR). It is activated by cyan light and ...

  2. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...Patrick Miller-Rhodes from the University of Rochester Medical Center. Patrick Miller-Rhodes is currently...This post was contributed by Patrick Miller-Rhodes, a Ruth L. Kirschstein NRSA Predoctoral Fellow at ...

  3. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...interneurons Isocortex 223971 pAAV-3xSP10ins-AiE0354m-minRho-SYFP2-WPRE3-BGHpA AiP12040 AiE0354m FlpO Vip interneurons...interneurons Isocortex 230101 pAAV-hsA2-AiE0219h-minRho-SYFP2-WPRE3-BGHpA AiP11712 AiE0219h SYFP2 Vip interneurons...interneurons Isocortex 208401 pAAV-3xSP10ins-AiE0354h-minRho-SYFP2-WPRE3-BGHpA AiP12039 AiE0354h SYFP2 Vip interneurons...interneurons Isocortex 163503 pAAV-hsA2-AiE0140h-minRho-SYFP2-WPRE3-BGHpA AiP11633 AiE0140h SYFP2 Pvalb...interneurons Isocortex 230099 pAAV-hsA2-AiE0086m-minRho-SYFP2-WPRE3-BGHpA AiP11580 AiE0086m SYFP2 Pvalb...interneurons Isocortex 230807 pAAV-hsA2-AiE0086m-minRho-iCre(R297T)-BGHpA AiP20171 AiE0086m Cre Pvalb interneurons...striatum Striatum 191717 pAAV-3xSP10ins-AiE0367h_C2-minRho-SYFP2-WPRE3-BGHpA AiP12514 AiE0367h_C2 SYFP2 Medium...

  4. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ... illustrated with my favorite microorganism (Rhodobacter sphaeroides!) at the ASM booth. As well as being...Lakes Bioenergy Research Center and a fellow Rhodobacter enthusiast, Tim also hosted me at his University...

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ..., Adam Cohen's lab presented Archaerhodopsin 3 (Arch) as a new rhodopsin based voltage indicator for use...plasma membrane. To obtain a more sensitive channelrhodopsin actuator for triggering action potentials ...lab further modified sdChr- a blue-shifted channelrhodpson from freshwater green alga identified by Ed...

  6. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ... by delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel... Plasmid tools for microbiome studies Channel rhodopsins for optogenetics Viral Vectors 101: Chemogenetics...

  7. Brain Armamentarium

    Type
    Collection
    ...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by Lamp5 interneuron-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by cholinergic neuron-targeting enhancer...

  9. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons...use viral prep in serotype AAV5.  Get the channelrhodopsin here Astrocyte specific tight affinity glutamate...

  11. Optogenetics Plasmid Collection

    Type
    Collection
    ...opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins, are pumps that can ... as an optogenetic tool is the widely used Channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii . ...cations to flow into the cell. Variants of channelrhodopsin have been engineered or identified to increase...

  12. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...Activation of these neurons via the channel Channelrhodopsin 2 (ChR2) reduced glutamate transmission from...mouse to deliver Cre-dependent expression of  Channelrhodopsin 2 (ChR2), a modified receptor protein that...

  13. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...://doi.org/10.1038/s41586-025-08877-4.  Two powerhouse tools combine for dynamic protein labeling By...to express either the optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the...

  15. Brain Initiative Collection

    Type
    Collection
    ...AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and ...AAV9 pAAV-hSynapsin- soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single millisecond... pAAV-hSynapsin-FLEX-soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single millisecond...pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal...pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST Anion channelrhodopsin GtACR1 fused to ER export signal and targeted...

  16. AAV Viral Preps

    Type
    Collection
    ...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...

  17. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...Veenman et al., 1992) or fluorescent molecules like rhodamine-isothiocyanate (RITC; Thanos et al., 1987) are...Vidal-Sanz M., and Aguayo A.J. (1987). The use of rhodamine-B-isothiocyanate (RITC) as an anterograde and ...

  18. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...simultaneously measuring the activities of CaMKII and RhoA during induction of structural plasticity in single...expression plasmids, FRET constructs, and CaMKII and RhoA sensors. Laviv, et al. Nat Methods. 2016. PubMed...

  19. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ..., reagentless biosensor for ADP based on tetramethylrhodamine-labeled ParM A fluorescent, reagentless ...reagentless biosensor for ADP based on tetramethylrhodamine-labeled ParM. ACS Chem Biol. 2010 Apr 16;5(4):415-25...reporter VARNAM Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator. Neuron. 2023... mammalian neurons using engineered microbial rhodopsins. Nat Methods. 2014 Aug;11(8):825-33. Adam Cohen...D95N), MacQ-mCitrine, QuasAr and QuasAr-mOrange) Rhodopsin-based voltage imaging tools for use in muscles...

  20. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...far-red fluorescent marker, a Flp-dependent channelrhodopsin in the retrograde serotype, and a dTomato ...
Showing: 21 - 40 of 72 results