Delivery Methods for Generating iPSCs
Type
Blog Post
...behind at the site of excision, this approach is still not considered safe for therapeutic applications...screening cells for the presence of transgenes is still an important control. Additionally, Sendai virus...The typical drawbacks of using transfection will still exist: transfection efficiency is cell-type dependent...by dilution with each cell division, but it can still take several passages for the minicircle to be completely... that ⅔ of subclones generated from EBNA1 iPSCs still had episome DNA present.
DNA-Free Methods
RNA...reprogramming proteins for 8 hours a week for 6 weeks, and still required further culturing to generate iPSC colonies...