...expression, though it is possible for the plasmid to still express without the G. Because the G is only one...the efficiency will be lower, although this might still be okay if you are introducing a selection marker...if/when you use HercII or Kapa Hifi the reaction still does NOT work, you can try these new primers: EMX1...

...but they still have potential targetable sites approximately every 35bp. Researchers are still determining...

...substrates and the ACP and SFP Synthases. Users may still generate their own fluorescent or non-fluorescent...

...years from symptom onset. Disease mechanisms are still poorly understood but several genes and mapped loci...

...mismatches toward the 5′ end distal to the PAM often still permit target cleavage. Cas9 undergoes a second ...desired edit, as a large percentage of DSBs are still repaired by NHEJ. How do you determine that your...

...For small deletions (as the “deletion band” may still amplify), resolve agarose gel to determine if size...

...Discontinued Penn Vector Core Viral Vectors (Plasmids still available, other serotypes may be available) Based...