We narrowed to 37 results for: t7

1. New England Biolabs Cell-Imaging Plasmid Collection

   Type

   Collection

   ...101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone for bacterial expression under T7 control 101135 pSNAP-tag...

2. Hot Plasmids - August 2020

   Type

   Blog Post

   ...The pMAK461-64 plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction...

3. CRISPR Antimicrobials

   Type

   Blog Post

   ... synthetic phages, each based on the well-studied T7 phage, to target various types of bacteria. In mixed...

4. Depositor Collections

   Type

   Collection

   ...MORF) Collection Synthetic Biology Voigt Fragmented T7 RNAP Plasmids Repressor Plasmids Sigma (σ) Factor...

5. Luciferase Plasmid Collection

   Type

   Collection

   ...firefly luciferase James Wilson 101156 T7-CMVtrans-FFLuc-polyA Firefly T7 Expression of firefly luciferase ...luciferase. Renilla luciferase is expressed from a T7 promoter for normalization. John Atkins 174050 pGWB-nLUC...

6. Finding nucleic acids with SHERLOCK and DETECTR

   Type

   Blog Post

   ...transcriptase (RT)-RPA, respectively. RPA is coupled with T7 transcription to convert amplified DNA to RNA for...

7. CRISPR Plasmids - Xenopus

   Type

   Collection

   ...pyogenes (PAM = NGG) 51306 pUC57-Simple-gRNA backbone T7 BsaI In vitro transcription none, need Cas9 plasmid...

8. Bacterial Expression Systems

   Type

   Collection

   ...(aTc) Escherichia coli Stanley Qi 11518 pDest-527 T7-lacO Lactose/IPTG Escherichia coli Dominic Esposito...Escherichia coli Cheryl Arrowsmith 26094 pET28a-LIC T7-lacO Lactose/IPTG Escherichia coli Cheryl Arrowsmith...Escherichia coli Andreas Moeglich 26092 pET15-MHL T7-lacO Lactose/IPTG Escherichia coli Cheryl Arrowsmith...

9. Plasmids 101: Repressible Promoters

   Type

   Blog Post

   ...be produced. Many commonly-used promoters, such as T7, CMV, EF1A, and SV40, are always active and thus ...

10. Technique: Probe Phage Genomes for Host Binding Proteins

    Type

    Blog Post

    ...DNA into E. coli BL21 (DE3) cells (these have the T7 polymerase and a few proteases knocked out making...

11. Sequencing Primers

    Type

    Guide

    ...promoter Forward T7 TAATACGACTCACTATAGGG T7 promoter Forward T7 Terminal GCTAGTTATTGCTCAGCGG T7 terminator ...

12. Promoters

    Type

    Guide

    ...Expression Description T7 Constitutive Promoter from T7 bacteriophage; requires T7 RNA polymerase Sp6 Constitutive...

13. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type

    Collection

    ...Tetracycline (Tet) Inducible Expression page Bacteria Lac, T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector...

14. Modular Cloning Guide

    Type

    Guide

    ...Freemont 78 plasmids including constitutive promoters, T7 expression, RBS strength variants, synthetic terminators...

15. 27 Hot Plasmids from 2016

    Type

    Blog Post

    ...Grunwald pCS2TAL3-DD, pCS2TAL3-RR Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination series, pCAG-Golden-Gate-Esp3I-Destination...

16. CRISPR Plasmids - Empty gRNA Vectors

    Type

    Collection

    ...yes, cut S. pyogenes W Fujii MSP1673 65769 Bacteria T7 yes, cut N. meningitidis Joung Cas9 sgRNA vector ...

17. Fluorescent Protein Guide: Empty Backbones

    Type

    Collection

    ...C-SNAPf , pCS2+/N-SNAPf - Xenopus Expression pSNAP-tag (T7) Vector - Bacterial Expression CLIP-Tag Benzylcytosine...