...Browse plasmids supporting the evaluation of a diverse set of novel Alzheimer's disease targets....targets in Alzheimer’s disease (AD) by generating validated resources for emerging targets in AD and creating...lead molecules for prioritized targets that are valued opportunities for further translational investment...

...These antibodies undergo application-specific validation and consistent quality control measures. We work...experimental data for recommended applications. Addgene values transparency and accessibility in research and ...Addgene’s collection of antibodies developed and validated for many research applications. Browse Antibodies...

...Zebrafish Burgess 61570 Pvalb-2A-Cre targeting vector Cre; Targeting vector for Pvalb locus Mammalian Zeng...PI 11578 pSico Cre activates shRNA expression, removal of EGFP; See also similar plasmids pSico PGK GFP...Jacks 11579 pSicoR Cre turns off shRNA expression, removal of EGFP; See also similar plasmids pSicoR PGK puro...These plasmids can be useful for lineage tracing, evaluating infection/transfection efficiency, selecting ...

...monoclonal antibodies undergo application-specific validation and quality control by Addgene as well as by ...antibodies currently available from our catalog. Addgene values transparency and accessibility in research. For...cross-species reactivity. Reactivity is based on validation data from Addgene or trusted partner labs or ...

...MUST be placed in an ice bucket immediately after removal from the -20 °C freezer because heat can cause ...Polymerase or Klenow DNA Polymerase I for 3′ overhang removal and 5′ overhang fill-in. If you are using blunt...

...adjustment etc. Consequently, each batch needs to be validated and the best ratio of mass DNA:mass PEI determined...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmid...

...digested vector backbone. This is accomplished by covalently connecting the sugar backbone of the two DNA ...enzyme. The DNA ligase catalyzes the formation of covalent phosphodiester linkages, which permanently join...

...inactive (GDP) state, regulated by the addition and removal of a phosphate group. Under normal physiological... signaling pathways regulating cell migration, survival, adhesion, growth, and differentiation. Schematic...genes involved in cell growth, differentiation and survival. Ras is essential for regulating the growth of...

...consider runs with an NTC > 10 to be invalid. To reduce NTC values, we recommend wiping down all pipettes...

... Knockout Mouse Chen N/A (AAV) 4 286 Mouse Validation (mVAL) CRISPR Library 159391 Knockout Mouse Moffat...Human Weissman 3rd 10 Varies CRISPR/Cas9-assisted Removal of Mitochondrial DNA (CARM) Library 82480 Knockout...

...Monitor the ATP:ADP ratio during live-cell imaging (Perceval) A genetically encoded fluorescent reporter of... ATP/ADP Fluorescent sensor of ATP:ADP ratio (PercevalHR) Imaging energy status in live cells with a fluorescent...endocytosis is the dominant mechanism of vesicle retrieval at hippocampal synapses. Neuron. 2006 Sep 21. ... of pHluorin Two pathways of synaptic vesicle retrieval revealed by single-vesicle imaging. Neuron. 2009...

...scientists who want to immediately start cloning upon arrival of their plasmid from Addgene - reducing the time...grade DNA prep, the plasmid sequence has been validated by next generation sequencing. The DNA concentration...

...generally ready for shipment within 8 weeks after the approval of the MTA by your institution. Our final step...order being shipped, Addgene can provide the QC validation data performed on the material received. Shipping...

...for research in Parkinson's Disease; For target validation and drug design Antibodies Addgene distributes...produced in-house and undergo application-specific validation and quality control by Addgene as well as by ...

...produced in-house and undergo application-specific validation and quality control by Addgene as well as by ...collection - monoclonal antibodies extensively validated for neuroscience research applications from the...

...factors, including insulin. mTORC2 influences cell survival and cytoskeletal organization. Cancer Impact Enhanced... to signal upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids Click...

...fluorescent protein) in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression. ...

... and ChR2-EYFP, a large number of additional, validated molecular payloads in the INTRSECT configuration... 2019. Mapping Brain-Wide Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex...

...answered using model bacterial systems and plasmids avaliable at Addgene. Read below and browse our curated ...Fluorescent protein, GUS, and LacZ Derivatives avaliable Philip Poole A family of promoter probe vectors...

... its maximum value. Maturation is the time for fluorescence to obtain half-maximal value. Structure describes...