We narrowed to 405 results for: pho;

1. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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   ...these TFs to see if the differentiated cells had morphologies consistent with the predicted cell type (e.g...neuron predicted differentiation), and 15/17 morphologically aligned. Documentation of the network that ...

2. Plasmids 101: Inducible Promoters

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   ...sensing protein YFI. When light is absent, YFI phosphorylates FixJ, which binds to the FixK2 promoter to ...

3. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   ...designed by the Toettcher Lab, OptoNBs are photoswitchable engineered nanobodies whose binding of their...cascade. This signaling is a lot like a game of telephone, until the message reaches the nucleus where gene...

4. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ...transmission within minutes. Constructs using the photodimerizer system CRY2-CIB1 to regulate botulinum neurotoxin...gas-filled protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins ...

5. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   ...tumorigenesis assays, drug epistasis assays, and morphological profiling can be used as generalizable strategies... are described in this paper Systematic morphological profiling of human gene and allele function ...

6. Multiplex Genome Editing with CRISPR-Cpf1

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   ...example of how this works. Make sure to order 5’ phosphorylated oligos or treat with T4 PNK. Delivery options...

7. Sweating the Small Stuff: Details in the Lab

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   ..., a large set of headphones can act as a Do Not Disturb sign, even if the headphones aren’t actually playing...

8. Antibodies 101: Flow Cytometry Controls

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   ...should have a single color control for every fluorophore you intend to use in your panel. Using single...have the same Ig subclass, and be in the same fluorophore (e.g., if you are staining for anti-mouse Ly6C...

9. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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   Blog Post

   ...inactivation maximum of 590 nm light. Aurora and Phobos optogenetic tools - A collection of bi-stable anion... medicine, evolution of body plans, synthetic morphology, and even artificial intelligence," says Michael...

10. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post

    ...selecting a bright fluorescent protein and a photostable fluorescent protein. In the third post of this...verify a number of key properties (brightness, photostability, oligomerization) of a number of promising ...