We narrowed to 625 results for: Emb

1. AAV Vector Quality Control: Going the Extra Mile with NGS

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   ...to de novo assembly of the unmapped reads, employing our bioinformatics software to assemble the reads ...

2. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   ...start life as fertilized eggs that develop into embryos and then become larvae. There are three larval ...importantly, this knowledge will stop you from embarking on a fruitless journey of trying to get males ...

3. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   ...this case the cell needs to be fixed and the cell membrane permeabilized in order for the antibodies to reach...Parton's Lab (University of Queensland) to observe membrane trafficking events in vivo. Locate your protein...

4. Tips to Make the Most of a Scientific Conference

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   ...break), write down some notes on the card so you remember who they are and what you’ve talked about. After... Resources on the Addgene website Become of a member of the Addgene depositor community ...

5. Magnetic Control of Proteins: More than a Dream

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   ... adaptable into future magnetogenetic tools.  Embracing science in the open The York Lab operates with...applications across a wide range of topics. He embraces open science, self-publishing directly on his ...

6. Plasmids 101: Degron Tags

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   ...associate with the endogenously expressed SCF complex members, an E3 ubiquitin ligase complex. Finally, when ...Chem Biol. 2015 Sep;11(9):713-20. doi: 10.1038/nchembio Nabet B, Roberts JM, Buckley DL, Paulk J, Dastjerdi...

7. Tips for a 1st time AAV user (by a Rookie AAV user)

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   ...aliquoting purified and buffer exchanged virus, remember to make one or two small aliquots to use for qPCR...Tips When calculating the titer for your AAV, remember to account for the dilution of the sample for ...

8. Light Sheet Fluorescence Microscopy

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   ...development of vertebrate embryos.  The first 3D reconstructions of clear embryos were obtained by using ...

9. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   ...else you’d like to say to our readers? Patron: Remember plants. Every time you look at your plate, think...Journal. A modular cloning system for standardized assembly of multigene constructs. Weber E, Engler C, Gruetzner...

10. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    ...likely due to quarantining {% video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist..._playlist=True, viral_sharing=False, embed_button=False, autoplay=False, hidden_controls=False, loop=False...

11. An Introduction to Adenovirus

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    ... derived from the mastadenovirus members (mammalian-infecting members) of the Adenoviridae family, which...

12. Course-Based Undergraduate Research Experiences (CUREs)

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    ...!). In 1997, Joseph Provost, then a new faculty member at Minnesota State (and now Professor of Chemistry...and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the recipe...

13. Tips for arabidopsis transformation

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    ...2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids from the Dinneny...Arabidopsis.html?id=IfZAMNPWVk4C&redir_esc=y (Accessed: 21 September 2018). Additional resources on the Addgene blog...

14. Plasmids 101: Colony PCR

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    ...want to know the orientation of the insert. One member of this type of primer pair anneals to a sequence...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org Read about...

15. Tips for Screening with Yeast Two Hybrid Systems

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    ...nucleus. To circumvent this requirement for transmembrane proteins, a split ubiquitin system has been ...genetic system optimized for systematic studies of membrane protein interactions. Proc Natl Acad Sci U S A...

16. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    ...sharing this protocol on the Addgene website in December 2006, the pLKO.1-TRC cloning vector (deposited... allows labs to create accounts in which their members can share protcols, sign-up for shared equipment...

17. Your Lentiviral Plasmid FAQs Answered

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    ...technically gamma-retroviruses - another, separate member of the retroviral family. While both lentiviruses...Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing and non-...

18. Negotiating Work and Life: How to Find the Joy

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    ...Maybe you’ll need to care for an elderly family member, want to train for a triathlon, finally buy that...laundry, take public transportation, call a cab and remember their own school assignments, the better. In my...

19. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...cleaving genomic DNA in mammalian cells and zebrafish embryos, and they can be used to modify genomic loci that...SpCas9 (Amrani et al., 2018). When choosing a Cas9, remember to check that your tracrRNA and crRNA (or synthetic...

20. Hot Plasmids - August 2020

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    ...with plasmids encoding FPs tagged with the plasma membrane targetin sequence derived either from p63(1-29...non-specific labeling. For labeling the plasma membrane, they tested different target sequences for improved...