We narrowed to 545 results for: SEC

1. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   ...viral packaging cell line. For example, Phoenix, a second generation γ-retrovirus packaging cell line developed...

2. Clear and Accessible Writing

   Type

   Blog Post

   ...the first time and learning how to purify AAV.  Second, you’ll identify your purpose in using that image...

3. Viral Vectors 101: Viruses as Biological Tools

   Type

   Blog Post

   ... into hard-to-reach organs, such as the brain. Secondly, some viruses have a low immunogenicity which ...

4. Sharing is Caring: But How to Distribute Open Hardware?

   Type

   Blog Post

   ...can modify and optimize the code, find and fix security-critical bugs, and contribute to constant updates...

5. How Addgene Creates Content

   Type

   Blog Post

   ... expert review, separate from the author, and a second review from someone less familiar with the subject...

6. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   ... usually with a fully fleshed out introduction section) to one of 222 participating journals before a ...

7. Plasmids 101: Methylation and Restriction Enzymes

   Type

   Blog Post

   ...of DNA Dcm methylase adds a methyl group to the second cytosine of CCWGG EcoKI methylase adds a methyl...

8. How to Keep a Lab Notebook for Bioinformatic Analyses

   Type

   Blog Post

   ...Alternatively, you can simply make a note in your methods section about the edit that was temporarily used. Just ...

9. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

   Type

   Blog Post

   ... so they won’t work if the fragment ends have secondary structure or too much sequence similarity. REPLACR-mutagenesis...

10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    ...tRNA-gRNA constructs or a 1-4 base tail from the second tRNA in the tRNA-gRNA-tRNA constructs. gRNAs with...

11. 3 Challenges in Plant Synthetic Biology

    Type

    Blog Post

    ...mechanisms – our project wouldn’t be possible. A second technical hurdle is the lack of resources associated...

12. Dos & Don'ts When Publishing a Scientific Manuscript

    Type

    Blog Post

    ...and software tools in the Materials and methods section of their manuscript to make resources easier to...

13. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    ...origin of replication (Please note: This first section primarily pertains to ORIs in budding yeast, Saccharomyces...

14. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type

    Blog Post

    ...finding someone to give you advice and guidance. Second, I will offer some advice on how to “make the ask...

15. Adenovirus Guide

    Type

    Guide

    ...vectors. Created with BioRender.com. Second-generation rAdV vectors Second-generation rAdV vectors contain ... is, by engineered packaging HEK293 cell lines. Second-generation rAdV vectors were shown to elicit less...first- and third-generation rAdV vectors. Figure 3: Second-generation rAdV vectors. Created with BioRender.com... vectors is normally transient and short-lived. Second- and third-generation rAdV vectors have been developed...number indicates the serotype of the genome and the second number the serotype of the capsid. Use of these...rAAV vectors (~4.7 kb) and is further increased in second and third-generation vectors. This capacity is ...

16. dTAG - You're it!

    Type

    Blog Post

    ...these plasmids are available here. The Methods section of Nabet et al., Nature Chemical Biology additionally...

17. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    ...was directed toward a protein of interest but the second antibody was directed toward AHA. Now the rolling...

18. Human Germline Editing Using CRISPR

    Type

    Blog Post

    ...edited cells. The results were even lower for the second gRNA: 13.1% targeting efficiency with only 13% ...

19. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    ...of 500 nm light (turn on psRaf1) followed by 3 seconds of 400 nm light (turn off psRaf1). After this, ...

20. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type

    Blog Post

    ...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...