...epifluorescence microscopy wasn’t able to give a clear view of cellular movement in real time. Two-photon...microscopy has uncovered host-pathogen interactions leading to the understanding of effector function in infected...Weninger. "Dendritic cell behaviour in vivo: lessons learned from intravital two-photon microscopy." Immunology... Get Tips on Choosing Your Fluorescent Protein Learn Some of the Pitfalls of Working With Fluorescent...

... the pPSU2 plasmids are available from Addgene. Learn How to Analyze a Restriction Digest References ...Baby Gel Box Find 5 Smart Phone Apps for Lab Life Learn About the Evolution of Lab Techniques Resources...Kit-Free Alkaline Lysis Plasmid Miniprep Protocol Learn How to Analyze Your Restriction Digests Make Your...

... company, Science with Tom. In this podcast, we learn more about Tom and pick his brain for advice on ...importance in understanding biological history. 4. Please Don't Kill My Hive Beautifully filmed video about...generation science standards and communicate science. Learn more about Tom on his youtube channel and at sciencewithtom.com...

...forward with these efforts, you’ll find new ways to learn about science careers, lab protocols, and, of course...read through each one, plug in some headphones to learn more about the latest and greatest plasmids through...Plasmid webpage. We hope you enjoy this new way to learn about plasmid technologies. If you have any comments...

...Ready-to-Use CRISPR Lentivirus Learn about Other Members of the Addgene Leadership Team ....     Additional Resources on the Addgene Blog Learn More About Alex's Work with Cas9 Activators Michael...

...journey is at an end! Their name is...(drum roll, please!)... Abi! Thank you so much to everyone who submitted...Which name best fits our new immunoglobulin friend? Please help us decide by voting for your favorite on this...Original Blugene and Aavery would like your attention, please, as they introduce their new friend…  Fig...

...Cambridge to spend some time learning and doing research at Novartis. Listen to learn how Kwabena plans to use...Addgene Blog Check Out Posts on Synthetic Biology Learn about the Addgene, Seeding Labs Plasmid Grant Read...

...to manage these changes quickly and harmlessly. Learn much more about the laws and their possible repercussions...School Advice Part 2: Building Community.” This post leads readers to our latest podcast all about graduate...to catch up on ways to use CRISPR in plants, to learn techniques for detecting genome edits, to discover...

...Since this is a beta project, it will run for at least 6 months as we assess the needs of the scientific...Browse blog posts from our Plasmids 101 blog series Learn about the different grades of DNA  Read about the...ways to quantify DNA Resources on Addgene.org Learn about our other beta projects Read our Molecular...

... Now, they’ve filled that gap. As Sakuma and colleague Takashi Yamamoto describe in their recent study...tandem. They went on to show that their CRISPR/Cas9-nuclease and CRISPR/Cas9-nickase vectors work successfully...Currently, the kit only includes two versions of Cas9, nuclease and nickase, but we are now adopting dCas9, dCas9...

...stress-induced biochemical changes that could otherwise lead to depression. And there you have it, mck-PGC-1a1...beneficial effects on the brain,” Ruas said in a press release. “We actually found the opposite: well-trained ... demands,” they write. “In the future, these may lead to treatments for a variety of brain disorders, ...

...corner of this post) is an example of a seemingly clean DNA sequence (no Ns in sight). If you never looked...like these…. 3. Expect to get 500-700 bases of clean reliable DNA sequence Anything less and you might...and small, so I suggest designing your primer at least 50bp upstream of the sequence of interest. 5. Use...

...Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids from the Kotton Lab Learn more ...mission to promote scientific sharing. Listen to learn more about Darrell, his research, and his focus ...

...Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal DNA cleavage at 48 ...included in the next What’s New in CRISPR blog post, please let us know! Additional resources on the Addgene...

...Attendees consistently mentioned how pleased they were to learn about these exciting scientific topics ... members gave up a beautiful summer Saturday to learn about science, but they didn’t seem to mind! Attendees...

...scared of taking risks, afraid of leaping forward but I have learned to push myself to the forefront, ...internship at Addgene. Although my interests are very clear, I had no idea how to translate them into real world...

...mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi and find plasmids...vegetables; Broccoli, Spinach, and Corn, for example. Learn more about the aptamer soup in this blog post. ...something you’d like to see more of? Let us know by leaving a comment or emailing us at [email protected].  ...

...spreadsheet of random numbers that’ll leave you, well, #sad. Learn More About CRISPR Pooled Library Screening...PMCID: PMC4744125. Resources on the Addgene Blog Learn to Conduct Genome-wide Screens with CRISPR/Cas9 ...

...Science Signaling in December 2014, he and his colleagues at Duke University, in collaboration with “cloning... of the cancer pathways ORFs kit Wood and his colleagues developed the kit for use in screening for pathways...PMC4353587. Additional Resources on the Addgene Blog Learn How the Clon Tracer Library Has Been Used in Cancer...

...scientists standing on the shoulders of their colleagues and all those who’ve gone before them. Given ..."The Secret of Life." 10)     And, last but not least (certainly not for us), Addgene launched this blog... Year, let's make a toast to open science. And, please, share your favorite OA developments of the past...

...openness and trust that comes from caring about your colleagues on a personal level, and employees often cite...project. These projects could be anything from cleaning out the freezers to writing new plasmid protocols... it? Perhaps new ideas or collaborations, or at least a shared sense that we’re not alone in struggling...

...planning, it shouldn’t be used in isolation. Without clear dialogue between trainee and PI, it’s easy to miss...Starting with a recap of accomplishments, from learning new techniques to finally figuring out a tough...the yearly planning meeting is that the trainee leads the conversation. As the conversation proceeds through...

...and easily. These tagged plasmids also include cleavage sites should one need to remove the tag post-purification...on these and other tags used in the pCri system, please see Plasmids 101: Protein Tags. Beyond E. coli...purification. For a more thorough introduction to pCri, please see the original publication cited below. pCri ...

...some Addgenies. As the gala came to an end, it was clear that the Addgenies had no intention of ending the...tough but memorable one. At the gala, one thing was clear: the sense of family is still the backbone [ba-dum-chh...helped to edit and add to this post. Interested in learning more about the last 10 years @Addgene Check out...

...materials from the Addgene repository. Read on to learn more.  What will be available?  Over 3000 AAV plasmids...AAV5, AAV8, or AAV Retrograde (AAVrg) capsids.  Please be aware that this ordering process will look slightly... the identity of the packaged DNA and serotype. Please note that Addgene cannot perform functional or ...

...universities and Technology Transfer Offices were, at least, temporarily closing, these groups were willing ... would like to make them available to industry, please reach out at [email protected] and we will...Addgene blog Browse blog posts related to COVID-19 Learn more about the Ginkgo COVID-19 plasmid collection...

... repressors of gene expression in yeast Megan McClean’s lab generated an optogenetic toolkit that controls...repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders the NLS...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

...blog posts Learn more about retrograde AAVs Resources on Addgene.org Read our AAV guide Learn more about...Understanding the biology behind brain development, learning, and behavior poses many challenges in neuroscience...

...method that uses iterative staining and chemical bleaching to allow for high-resolution imaging without degrading...the IBEX Knowledge-Base is named on the Zenodo release in keeping with their value of shared ownership...IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues...

...nucleus in space and time, and to understanding how nuclear organization varies across the life of a cell, ...dCas9 gets a colorful makeover Even without its nuclease activity, catalytically dead Cas9 (dCas9) has ...under lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the...