We narrowed to 873 results for: LIC

...throughout the lifespan of the fly to determine the implications of your genetic manipulations. You can produce...immunofluorescent images like in this paper (2). Metabolic assays such as Seahorse can be employed to look...

... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ... PubMed Central PMCID: PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene promoter confers...

... this stage and that can be used to simulate in silico cloning. This will definitely help in understanding...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...

...modular suite of plasmids (pGT) with various replicative origins that can be used for broad or narrow ...Central PMCID: PMC5963681.  Meyer, Richard. "Replication and conjugative mobilization of broad host-range...

...generate dsRNA as part of their infection and/or replication process within the host. dsRNA viruses are inherently...generate dsRNA intermediates as part of the viral replication process. dsDNA viruses yield dsRNA through a ...

...Deficiency (CTD) is a rare X-linked inherited neurometabolic condition and one of three Cerebral Creatine... into CCDS. The brain and muscles have a high metabolic demand that is powered by ATP. A molecule central...

...IgG2 or IgG4. This can be important for some applications. Assay use While secondary antibodies are useful..., the flexibility of their use varies across applications. Western blots, for instance, almost always ...

... Haoyi Qiu He Tian Every two years, we seek applications for awards to early career scientists using ...virtual conference attendance. We also opened up applications to research technicians and undergraduate researchers...

...Each half of the base editor is fused to a fast-splicing split intein and co-infected into the cell. Intein...increasingly been recognized for its biotechnological applications including conversion of CO2 into water and electrical...

...especially if you start to consider clinical applications). But there are a few other classes that we ...proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020; Niemeyer, et al., 2007...

... as secondary antibodies, have much broader applicability. It is fairly easy to find these in a Fab fragment...fragments described above can be used for similar applications and have similar advantages, though with some...

...   With the addition of blue light, the terminal helices of the LOV2 domain unfold, disrupting the protein...response to blue light (445nm). The discovery and applications of protein partners that bind to only the light...

...reached at high power may not translate to real applications in which far less power is used. For example...insight into the photostability in the intended application (Goedhart et al, 2012). The third issue relates...

...were at institutions where no data had been made publicly available (below). However, on November 22nd a...compelled at the state level, in the case of some public institutions) to cancel plans to raise salaries...

...can fail but, in our current setup, there are no explicit instructions on what to do when something fails... We can set ourselves up for success by giving explicit instructions to lab members through the software...

..., excellent resources with effective gRNAs are publicly available, including those found at Addgene! ...realization of a variety of perturbations and applications in functional genomics, cell reprogramming, ...

...and exciting applications, this system is an incredibly powerful tool for cloning complicated constructs...

...looking for a particular protocol or are trying to replicate an experiment, the methods section probably won...is science’s way of validating work prior to publication, it doesn’t mean that there are no mistakes or...

... perspective and you can layer them for more complicated functions. Those things are easy with CRISPR....just using what biology has given us. Wood is a complicated material and we can access it, but we are stuck...

...important plasmid elements – promoters, origins of replication, protein tags, and antibiotic resistance markers...proteins or co-factors, thus allowing for broad applications in a variety of experiments. LoxP (locus of ...

...potential to be used for other types of CRISPR applications. Let’s take a look at the key components of ... which means they are non-infectious and non-replicative. Nanoblades are produced by transfecting the ...

...important to consider that the same receptor can elicit different responses depending on what cell type...chemogenetics for research and potential clinical applications. Science 6436: eaav5282. https://science.sciencemag.org...

...BioRender.com In their recent ACS Synthetic Biology publication, Mary Dunlop’s lab generated a photosensitive...regions with Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes bringing the C-terminal...

...Jerome Goudeau, Cynthia Kenyon, and colleagues at Calico Life Sciences. Like many split FPs, split-wrmScarlet...Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express hybrid ...

...viral particles that have the gene deletion could replicate when challenged with the S. islandicus CRISPR-...B, Bondy-Denomy J (2020) Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies....

...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts...

...experiment and they don't do anything. Viruses are complicated and they perform complex biological tasks, both...vector may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try amplifying...

...rho-dependent and rho-independent. Rho factor is a helicase which assists RNA polymerase in the termination...Central PMCID: PMC104046. Wodrich H, Schambach A, Kräusslich H-G. Multiple copies of the Mason–Pfizer monkey...

...typically requires transducing with a limiting multiplicity of infection (MOI) to ensure only one copy integrates...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...

...these people and bios were likely part of the publicity. Good panels do not waste time on long introductions...on 1-2 panelist for each question in a way that elicits those interesting stories and opinions. Don't let...