We narrowed to 732 results for: SON

1. Hot Plasmids and Viral Preps - September 2021

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   ...stop codons.    New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

2. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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   ...recombination in samples. For a more detailed comparison of these cloning strains, browse the table of...

3. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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   ... light.   Inspired by @UriAlonWeizmann, here my song about our paper recently published in @nchembio ...

4. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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   ...she will start her own research group at Boyce Tompson Institute.  References Baldwin, Melinda. "Credibility...

5. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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   ...Resources on Addgene.org Learn about the Michael Davidson fluorescent protein collection Check out Addgene...

6. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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   ...introduced at ~2 copies per cell per color, following a Poisson distribution. To overcome the limited expression...

7. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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   ...purification of tTALE-eGFP and tTALE-mCherry. In comparison to intercalating fluorescent dyes which can cause...

8. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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   ...strands that are packaged at the same frequency, we reasoned that, following DNA extraction, the [+] and [-...

9. New and Upcoming Viral Vectors - December 2019

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   ...Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 124650 AAV9 pAAV-CamKIIa-C1V1...

10. Antibodies 101: Epitope Availability

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    ... Rachel Leeson...

11. Viral Vectors 101: Transductions

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    ... will not stably integrate (unless you used a transposon system, CRISPR targeted strategy, etc.) and episomal...

12. Multifunctional Neural Fibers: Modulation from Gut to Brain

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    ...the gut-brain axis (admittedly for self-serving reasons – I want to understand my obsession with the combination...

13. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    ... Rachel Leeson...

14. Antibodies 101: Epitope Tags

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    ...they exist but are unreliable for a variety of reasons (Baker, Nature). Tagging your protein allows you...

15. Antibodies 101: Chimeric Antibodies

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    ...     Morelock, M. M., Rothlein, R., Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R.,...

16. Plasmids 101: TOPO Cloning

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    ... Lianna Swanson...

17. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    ...shift won’t happen overnight, but there are a few reasons why you might choose NgAgo over CRISPR proteins...

18. Plasmid Cloning by PCR

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    ...to add overhangs to your insert of interest for Gibson assembly. The steps following primer design and...

19. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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    ...functionality of the RNA scaffolds follows the rules of Watson-Crick base pairing, allowing for rational, straightforward...

20. Hot Plasmids: FRET-Based Biosensors

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    ...contribution to the field is a series of fluorescence resonance energy transfer (FRET)-based calcium biosensors...

21. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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    ...maybe we could treat patients suffering from Parkinson's, chronic pain, deafness, or other conditions....

22. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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    ...be more difficult in the virtual format than in-person. You might miss out on the short chats between ...

23. New and Upcoming Viral Vectors - September 2019

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    ... neurons near the target site.” pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (124651-AAV9) Recombinase AAV...

24. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    ...reporters have been developed, such as the Forster resonance energy transfer (FRET)-based executioner caspase...

25. The Crispr Babies Saga Shows the Need for Action, Not More Delays

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    ...the research has been adequately proven to be reasonably safe and effective. We do that through a standardized...

26. Scientists Map the SARS-CoV-2-Human Interaction Network

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    ...takes time away from our research. For all these reasons, the QCRG is delighted to be partnering with Addgene...

27. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    ...the mouse CRISPR knockout library at Addgene. Henriksson et al., Cell, 2019. PubMed PMID: 30639098. LipoGlo...

28. The MTA at Addgene

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    ...  Rachel Leeson...

29. Hot Plasmids - January 2023

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    ...reproducibility of a (recombinant) monoclonal. Another reason is that some antibodies may just not perform as...

30. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    ...26808669. PubMed Central PMCID: PMC4916159. 2. Jacobson, K., Rajfur, Z., Vitriol, E., & Hahn, K. (2008...