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We narrowed to 1,076 results for: TOR;

Showing: 571 - 600 of 1076 results

  1. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ... first step. Developing mentoring relationships, especially with peer mentors, to discuss your strengths...recently, I have been hearing exit survey data from postdoctoral programs in the Boston area that demonstrate...Both explicit and implicit aspects of today’s postdoctoral training can directly interfere with a seamless...

  2. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ... able to eliminate the need for axenic (pure) laboratory cultures to study these microbes. From 16S rRNA...included a voltmeter on top of the battery box to monitor charge status and a disconnect switch between the... the battery bank and the vehicle battery/alternator to make sure the vehicle battery didn't run down.... can enable applications where reagents must be stored at elevated temperatures for long time periods ...

  3. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ... CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over 90% in ...such as ApE or commercial workbenches such as MacVector or Benchling. Utility functions: These functions.... For this reason I generally recommend that laboratories that have already attempted several CRISPR experiments...above or, even better, first-hand knowledge from laboratories employing CRISPR on the same organism could ...CRISPRseek offers great utility and coupled with Bioconductor should be the first choice for anyone already...

  4. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ... at 230 nm. The A260/A280 ratio is used as an indicator of DNA purity. Ideally, this number should be ...Concentration (ug/ml) = A260 reading x dilution factor x 50 ug/ml This method is quick and simple and ...like a PCR product). This method also gives an indicator of DNA or RNA contamination based on the presence...protocols and tips Find the best way to elute and store your plasmid DNA Learn about the different grades...

  5. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...Tools for checking grammar and jargon Hemingway Editor  This is a free web-based tool for detecting the... saved to your Mendeley library. The library is stored in the cloud, so you can access it from anywhere...you organize and cite papers. With the Zotero Connector, papers can be saved from Firefox or Chrome browsers...with others which makes it easier to work with collaborators. Zotero is a desktop based program that’s compatible...

  6. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...University, and did postdoctoral work at Harvard Medical School and the University of Toronto. He is an author...quick turn-around time of Sanger sequencing have historically matched the needs of most molecular biology ...next generation (NGS) sequencing have prevented laboratories from using these methods for routine processes...

  7. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...none are lost. Each Addgene library has its own depositor transformation and amplification protocol, but... a file in FASTQ format, a standard format for storing NGS reads and their corresponding sequencing quality...from the count_spacers.py file from Feng Zhang’s laboratory. There are a number of other analysis scripts...screening, and if you have any tips, input, or stories of interest about library use, let us know in the...

  8. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...imaged together, you will need to consider the same factors as when choosing an individual fluorescent protein...previous blog post for more discussion of these factors). In addition, you will also need to choose fluorescent...infrared fluorescent proteins require biliverdin as a cofactor and so you may need to supplement your cells with...received his PhD in biophysics from UCSF in the laboratory of Ronald Vale, after which he was a fellow at...

  9. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...creating a time-stamped research plan in a public repository before conducting the experiment (note: registrations...participating journals before a study is conducted. Editors and reviewers recommend changes, and if everyone...position of having robust, null findings that are notoriously difficult to publish.  With Registered Reports...implement authentic scientific research and as the Director of Advising in the Division of Life Sciences at...

  10. Hot Plasmids - May 2022

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...comparison to the 62-90% error rate seen with Cas9 editors. The lab believes that this system could potentially...Development of a cleavage-free dCas9-based gene editor using microbial SSAPs. Image from Wang, et al, ...live cells remains a challenge due to multiple factors, including limitations of number of flourescent...

  11. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...shared the paper with my lab since one of the AAV vectors in the paper seemed like it could help one of my...conference, whether you are attending in person or monitoring that conference via social media. The podcast...BLACKandSTEM Twitter community. Dr. Page shares her story of how one seemingly innocuous tweet would not only...you advance your research or career? Share your story in the comments below!   Additional resources ...

  12. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...light activation, you can’t use fluorescence to monitor processes directly affected by light. Long-term...To make luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka University...Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns... Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing Nano-lanterns...

  13. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ... a novel TAL effector nuclease (TALEN) facilitates efficient knockout of HIV co-receptor CCR5. Nucleic...This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta. If you’... out of frame! Scott Findlay is currently a Postdoctoral Fellow at the University of Alberta. He is interested...

  14. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...nanobody. As a proof-of-principle the Toettcher Lab monitored how AsLOV2 insertion into mCherry or GFP nanobodies... Sevenless (SOS), a guanine nucleotide exchange factor that activates Ras. They expressed this OptoNB-...purified. More OptoNBs that bind cell signaling receptors would offer more levers to pull to control signaling... example, if the OptoNB could bind and block a receptor or a ligand’s activity, then a flip of a light...

  15. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...screening overview blog post.) *Note: Two Popular Vectors for Blue White Screening Available at Addgene Are... plasmid, creating an α-complementation cloning vector. When a cloning reaction goes to plan and your ...pUC18 and pUC19, and pBluescript are a few common vectors. Blue-white screening is just that – a screening...insert, there are other methods. Positive selection vectors encode a gene which, when expressed, is lethal ...

  16. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ..., one that is not food, not medicine, and not mandatory – people can decide for themselves if they want...comprehensive plant cloning kits available in the repository, MoClo and GreenGate, and we are excited to see...how you were able to overcome them. Share your stories in the comments below! And if you have developed...duty to pursue a degree in a synthetic biology laboratory and received her M.S. from CSU in 2014. Outside...

  17. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...help win a business plan competition and advance investors to the next stage of diligence." In business competitions...non-scientific audiences. "There are 100 ways to tell a story - convince your audience you are doing amazing things...crowd funding, federal funding, foundations, and investors are examples of various sources of funding. Always... Biology & the Importance of Assembly Standards Story of a SynBio Startup: RevBio's Epiphany (or Lack ...

  18. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...Addgene.org Browse all viral vectors available at Addgene Visit our viral vectors guide Find our chemogenetics...makes them light-responsive. When light shines on excitatory channelrhodopsin-expressing neurons, the channelrhodopsins...non-invasive methods to the neurons in the right secondary motor cortex (M2). Since M2 is located on the surface ...

  19. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...support team, is a safer bet than one from a large distributor that may not have direct experience with the ...decision by price point, shipping time, or other factors. Just remember that these are secondary considerations...specificity, and experimental considerations - no other factors can make up for an antibody that doesn't work for...that application validation is the most important factor in antibody selection, but what does that mean?...

  20. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...into mammalian cells, especially when fused to effector proteins for gene activation or gene editing. ... efficient Cas system with very compact genome editors and a gRNA down-sized by almost 40%. The activity...architecture and amino acid sequence of the prime editor, yielding PEmax, to work synergistically with epegRNAs... within the FAH gene was precisely corrected, restoring expression in the liver.  Read the paper in Nature...

  21. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...Engineering gut microbes in situ While the ability to monitor the microbiome with sequencing is vast, the ability...upon the Yeast Toolkit by replacing the backbone vector’s high-copy number bacterial ColE1 origin with the...origin with broad host-range (Meyer, 2009). The vector retains the oriT for delivery into recipients by... Counting cell division in the gut microbiota Monitoring microbial population dynamics in the gut can ...

  22. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...New Under The Sun Blog and is part of her larger tutorial series, A Beginner’s Guide to Writing Scientific...when I can’t it’s an easy search. Writing this tutorial has shown me that I’m still in the dark ages with...guest blogger! Johnna Roose is a biochemistry instructor in the Department of Biological Sciences at Louisiana...Manuscript by Maaike Pols, PhD, a Developmental Editor at F1000Research  ...

  23. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...student and I truly enjoyed learning.” Addgene’s Director of Product Management Eric Perkins, who has been...you’re set up for failure. Listening to Kenkel’s story has helped me realize that is not true at all. Racheal...Komuhendo, a Lab Technician, dreamed of becoming a doctor. After taking a year break in between undergraduate...interviewees. Fabienne Furt, Addgene’s Associate Director of Business Development, got her PhD in Plant ...

  24. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ...beautiful figures. And, you can’t wait to tell your story. But, before you can show off your work at a conference...design was conceived by Mike Morrison, a psychology doctoral student at Michigan State University. The idea... center of the poster in a large font. Hence, spectators can read the text from many feet away and immediately...be the focus of your poster - the data and your story. If you choose a single-color background but want...

  25. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...neural network, and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle tissue as ...depleted via RNA interference (RNAi). Scientists monitor the effects of RNAi using four-dimensional contrast...assembly line for the creation of CRISPR targeting vectors that each contains a guide RNA and repair template...  Schwartz, M. L., & Jorgensen, E. M. "SapTrap vectors for introducing point mutations with unc- 119 +...

  26. A Look at Addgene's QC Process

    Type
    Blog Post
    ...compared to the information provided by the depositor. If a depositor provides a sequence for the plasmid, QC...collection. This standardization provides security for requestors, ensuring that the plasmid they ordered contains... elements based on the information provided and vector type  Any tags, selectable markers, and fusion ...

  27. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    ... infections along with a mild or exacerbated inflammatory response which leads to nearly 25% child mortality...understood.  In line with this, Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG...discovery led them to investigate the use of a common laboratory chemical, guanidine hydrochloride, as a potential...plasmids from Addgene to construct NFIX expression vectors as well as reporters of NFIX function. They will...

  28. CRISPR in the Clinic

    Type
    Blog Post
    ...potential for treatment is no longer an accurate descriptor; progress in clinical therapy is more fitting...suite, with Beam Therapeutics initiating a base editor-based clinical trial for SCD in 2022 and Editas... muscular dystrophy, cardiovascular disease, inflammatory diseases, blindness, and diabetes. Directions...Plasmids and Resources CRISPR 101 eBook CRISPR History and Development Resources on the Addgene blog...

  29. 6 Tips for Grant Writing

    Type
    Blog Post
    ...screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor/ a previous supervisor / someone...the field. 3. Highlight your network and your collaborators It’s a good idea to move around to different...Networking for Scientists Series 4. Become a science communicator More and more fellowship applications are asking...on your grant application to your peers and collaborators can also help you get some critical insight ...

  30. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...subcloned (using FX cloning) into the expression vector pSb-Init, which attaches a Myc-tag and a His6-tag... and isolate sybodies targeting the SARS-CoV-2 Receptor Binding Domain found on the membrane-bound spike... Synthetic nanobodies targeting the SARS-CoV-2 receptor-binding domain. BioRxiv.  https://doi.org/10.1101....16.045419.  Wrapp D, De Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam W, Roose K, van Schie L...
Showing: 571 - 600 of 1076 results