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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 921 results for: Tro;

Showing: 571 - 600 of 921 results

  1. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...interaction is not covalent, later work showed that introducing a Leu28Cys mutation in eDHFR does lead to covalent...2). If you’re working with purified proteins in vitro, you can even use the same tag for multiple proteins... actin filament assembly-regulating proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled...

  2. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...happy cloning! This article was written by Susanna Stroik and Meghan Rego, with thanks to Rob Hurt for his...Plants Mediated by a Cas9 Gene Containing Multiple Introns.” Plant Communications, vol. 2, no. 2, Elsevier...Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold Spring Harbor ...

  3. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...substance (fluorophore) that has absorbed light or electromagnetic radiation at another wavelength (Heinrichs,...Heinrichs, 2009). When a fluorophore absorbs light, electrons are elevated from a ground state to an excited state...After a brief elevation in this excited state, the electron returns to its ground state by emitting a photon...

  6. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español! Esta traducción... aumentar el acceso global a los materiales de nuestro repositorio. Este increíble regalo permitirá que...

  9. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...not just for a better day at the office but for stronger science as well. This blog is our way of continuing...Principal Investigator. How-to guides for troubleshooting your experiments and getting better results...

  10. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    ...here! Podcast Breakdown (Time: Topic) 0:00-1:00 : Intro 1:01-3:48 : Kingdom Kwapata's background and education... : Kingdom's Thoughts on his time in Boston and Outro We'd like to thank Dr. Kwapata for taking the time...

  11. Summer Fun at Addgene!

    Type
    Blog Post
    ...tricks for sharing your science with others, and introducing science communication as a research field. And...probably are familiar with our 101 series, which introduces common techniques and concepts. But what if you...

  12. Our New Antibody Mascot is....

    Type
    Blog Post
    ...some brand-new Addgene swag!   Last month, we introduced you to Blugene and Aavery's newest friend, our...Aavery would like your attention, please, as they introduce their new friend…  Fig. 1: Meet...well,...

  14. CRISPR Cheat Sheet

    Type
    Blog Post
    ...bacteria that allow the bacteria to target and destroy viruses that infect them. Commonly referred to ...With a repair template, CRISPR can be used to introduce a functional change in the DNA sequence. Base ...

  19. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    .... Not all of these technologies were without controversy, and it remains to be seen how popular any one...our most requested plasmid, SpCas9 is still going strong as the basis for many genome editing experiments...

  20. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...the general public that provides an accessible introduction to various scientific topics. Over twenty graduate...the audience engaged. The day kicked off with introductory lectures targeting three broad concepts: the...

  25. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...at eight different states of assembly from cryo-electron microscopy, the team reveal the mechanism behind...genome editing by fusing Cas9 to a mutated human estrogen receptor. This protein fusion stays in the cytoplasm...

  26. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...through any search, ordering, technical, or quality control issues. Improving customer support with self service...re looking for it, or you may just prefer to troubleshoot yourself without contacting someone else and...

  27. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    .... The kanamycin antibiotic resistance gene is controlled by a weaker constitutive Nos promoter, and it...Transgenesis with GreenGate Plasmids Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More Plasmids...

  28. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...worth it. Read here to get help if you are having trouble preparing your most excellent resume. 2) Follow... the criteria. Note the criteria that you meet strongly and note the ones that you could meet with learning...

  30. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ... sure to include various positive and negative controls to “convince [any] old skeptic professor who's...conference is a big multidisciplinary one.” Cepko strongly emphasizes that people should “follow their passions...
Showing: 571 - 600 of 921 results