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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 643 results for: ato

Showing: 571 - 600 of 643 results

  4. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...dCas9, zinc finger proteins, and transcription activator-like effectors (TALEs), with fifteen total variants...

  8. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...introduce or remove restriction sites), in mapping of regulatory elements (to mutate promoters/enhancers in reporter...

  9. Evolution of Lab Techniques

    Type
    Blog Post
    ...DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly progress...

  16. AAV Production in HEK293 Cells

    Type
    Protocol
    ...before putting the CS5 in the incubator. Return the CS5 to the incubator for 96 h. This incubation time...Hemocytometer or cell counter Ice bucket CO 2 incubator Pipet controller Hazardous waste container pH ...pH meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents Adherent HEK293T cells (...media and mix. Seed all cells in 1 CS2. Return to incubator for 48 h. Trypsinize and resuspend cells from ... with a total volume of 850 mL. Return to the incubator for 24 h–36 h. Proceed with transfection: Calculate...2% HI-FBS. Mix well. Take the CS5 out of the incubator and pour the media into a waste container. Carefully...evenly between the layers. Return the CS5 to the incubator for ~72 h. Harvest cells and media by tapping ...

  18. Antibodies 101: Validation

    Type
    Blog Post
    ...may not be the most accessible option for many laboratories. Additionally, it can be difficult to distinguish...

  23. Typing CRISPR Systems

    Type
    Blog Post
    ...Samai, P., Pyenson, N., Jiang, W., Goldberg, G. W., Hatoum-Aslan, A., & Marraffini, L. A. (2015). Co-transcriptional...

  28. Protocol - Bacterial Transformation

    Type
    Protocol
    ...bacterial colonies. Equipment Shaking incubator at 37 °C Stationary incubator at 37 °C Water bath at 42 °C Ice...temperature and then (optional) incubate in 37°C incubator. Mix 1 - 5 μl of DNA (usually 10 pg - 100 ng) ...antibiotic) to the bacteria and grow in 37°C shaking incubator for 45 min. Pro-Tip This outgrowth step allows... do this you will need to have access to an electroporator and the appropriate cuvettes. Follow the manufacturer's...

  29. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...personal protective equipment including laboratory coat, laboratory goggles, and gloves. Sodium azide containing...Class II, Type A2 Biological Safety Cabinet 4 °C Refrigerator Pipette controller Benchtop centrifuge compatible...tubes NanoDrop spectrophotometer 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead...upright at -20 °C Procedure Section 1: Affinity chromatography Equilibrate filtered tissue culture supernatant...

  30. Deep Dive: qPCR

    Type
    Blog Post
    ...19246619.Downey N. Interpreting melt curves: An indicator, not a diagnosis. IDT, 2014. Dwight Z; Palais ...
Showing: 571 - 600 of 643 results