Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,029 results for: ELL

Showing: 761 - 800 of 1029 results

  1. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...expected to make sure the work gets done and done well. Involving your team effectively is now your new... in mind, you won’t be able to communicate these well to the task assignee and this is a recipe for bad...delegating is a bad idea. How do I make sure it goes well? As with most managerial responsibilities, good ...Delegating" in the Harvard Business Review. Another excellent blog on delegation tips and practices can be found...

  2. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ... be wondering how this all fits in with science. Well, I’ll give you an example. At my previous company... goes into software product management? I always tell people the best part of my job is getting to talk...communicate with customers, software developers, as well as team members in automation, sales, marketing,...help you be a valuable asset to the scientists as well as the developers. Lastly, you’re going to need ...

  3. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ... R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Screen identifies... R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Rapid and phosphoinositol-dependent... Rando OJ, Xue Y, Swiderek K, Kuo A, Crabtree GR Cell. 1998 Nov 25. 95(5):625-36. An architectural role..., Ensminger AW, Fox AH, Chess A, Lawrence JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions...

  4. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...idea from academia to a startup Mike Koeris and fellow co-founders Tim Lu and others first came up with...non-scientific audiences. "There are 100 ways to tell a story - convince your audience you are doing amazing...aligned in vision and outcomes. The team must work well together, have complementary skill sets, and “talk...were free and free to repair.” If the team works well, the idea is solid, and “the pitch is packaged to...

  5. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...you correct any language- and spelling mistakes. A manuscript that is well written looks more professional...submission, missing files or a manuscript full of spelling errors will not have a great chance of getting...manuscripts submitted for publication. Holds a PhD in Cell Biology and a Masters degree in Infection and Immunity...

  6. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...practical solutions.  My PhD prepared me remarkably well for this task. Good scientists learn how to communicate...to say “I’m going to pour these plates now” to a fellow team member. But, at Addgene, we use seven different...different types of “plates” (Fig. 1). When you have to tell an engineer that you want the LIMS software to create...It is never really done. But if you implement it well, here’s what can happen: More effective meetings...

  7. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...enable scientific reproducibility. This workshop was well received, and then when the team started brainstorming...widespread issue of irreproducibility. Use your network well to seek out valuable and impactful opportunities...course! Thanks to individuals in the group who were well connected, this diverse and international team assembled...website How-to-guide for Reproducibility workshops Levelling Up: Reproducibility for Everyone Addgene: a global...

  8. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ... channels in living cells. Multiple properties of a channelrhodopsin, such as cell localization, kinetics...would result in a functional channelrhodopsin, as well as predict the channelrhodopsin’s photocurrent strength...minimally-invasive optogenetics While ChRgers performed well in a tissue culture dish, that doesn’t guarantee...

  9. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...read a 100-page genre fiction novella as it does to read an 11-page Cell paper - and sometimes, it might...obtain?” If the answer is yes, you will need to spell out that connection for your audience. I ask myself...needed. The trick is to communicate your key points well enough that the audience can take that knowledge...

  10. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ... project aimed at understanding how a critical cellular pathway is regulated. You’ve read all the background...generated against a linear antigen tend to work very well for applications that denature a protein to its ...expression in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody...References Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T,...

  11. Addgene: The First Twenty Years

    Type
    Blog Post
    ...Facebook and Gmail, were launched. And Melina Fan, a cell biology graduate student, was frustrated by the ...to receive the Massachusetts Nonprofit Network Excellence Award for our electronic MTA system. We also ... our most popular lentiviral and AAV vectors, as well as developing and sharing supportive educational...stories we can, and to hear the stories others have to tell.  "Also thanks for being Addgene. You guys rock....

  12. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...teams continue to keep up with the demand with excellence and dedication. We don’t have worry or wonder...right? Upgrades to the internal LIMS pages and excellent work on our external website appearance and functionality...support. The Addgene management team leads with excellence. They are one of the main reasons I have so few...Addgenies. I never worry about their dedication, excellent intentions, or incredible drive to succeed.  Thanks...

  13. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...transfer 100 µl of glycerol stock to each of 2 96-well microplates (identical copies, see image above)....integrates our database of over 2000 primers. Two 96-well PCR plates are prepared with the primer stocks for... be found here. Finally, you will want to have a well-organized database of expected sequences, either...some tips on how to get better sequencing reads? Tell us your favorite sequencing tricks in the comments...

  14. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...colony.  You should genotype all of your F1 mice, as well as progeny from future generations. 4. Continue ...strain, the promoter being used should be tested in cell lines to determine how it impacts the expression...on separate chromosomes in future generations as well, a concept known as linkage. This hinders the likelihood...interest. Cre may be expressed globally (in every cell of the mouse) or in a tissue-specific manner (with...

  15. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ... Fragmid website, or through Addgene, has worked well in the hands of the Doench lab. Additionally, controls...Drosophilia Plasmid pRDB_051 (for stable fly cell lines; attL and attR sequences for genome integration...Drosophilia Plasmid pRDB_052 (for stable fly cell lines; Hygromycin resistance gene; for genome integration...rapid assessment of emerging CRISPR technologies. Cell Genomics, 4(3), 100519. https://doi.org/10.1016/...

  16. Engaging with science and society at pgEd

    Type
    Blog Post
    ...communication, and policy. In this post, I hope to tell you a little more about how I ended up at pgEd, ...starting conversations about both the benefits as well as the personal, social and ethical implications...broaden my training beyond the biological sciences, as well as to acquire leadership and communication experience...scientific, educational or industry conferences, as well as schools, community events, churches or mosques...

  17. Tips for Technical Support Calls

    Type
    Blog Post
    ...answering help emails and phone calls, I feel compelled to share my years of accumulated wisdom so that...company’s FAQs? A lot of companies and organizations selling biological reagents have a ton of data on the catalog...their products from you, unless they’re knowingly selling a bad product (in which case, you should probably... some time. If the customer service rep does not tell you upfront how long it will take to get an answer...

  18. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ... on how your virus of interest causes cancer, as well as therapeutic strategies to target it, than you...at an unfamiliar venue. Finding your poster board well ahead of time ensures you aren’t scrambling to tack...published papers or “hot topics,” so be sure to be well-read on the recent literature in your field. Take...know every answer – don’t hesitate to be honest and tell your audience just that. Explain what you do know...

  19. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...uses a small molecule, a tag, and the endogenous cellular degradation machinery to function. The dTAG is...tag and small molecule. It does work in mammalian cells, mice, and even yeast, though (we have a kit!). ...and complete degradation of proteins in mammalian cells. Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350...Tags Plasmids for Endogenous Gene Tagging in Human Cells Antibodies 101: Epitope Tags  ...

  20. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ... thought about management skills before being propelled into the position of manager. I barely had any...check myself sometimes to make sure I am delegating well, but it does get easier over time! Communicate often...to recognize someone’s hard work and reward a job well done. Your report will (needs to) feel valued. Talk...should help you work through your situation, not tell you what to do. Unfortunately this is not always...

  21. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...is an excellent resource that has gained a lot of traction with researchers. It is an excellent way to... future career. If it wasn’t something I liked – well, I was used to experiments not working, so just ...programs and departments Graduate school was an excellent time to try new things, develop new skills, and...

  22. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...his own biases--many of which will be shared by fellow scientists--but that doesn’t make his introduction...have outgoing personalities to supplement their intellectual prowess and scientific skills. This is an era...has been such a great fit for me!), the book was a well-executed overview that recontextualized some areas...career.  Acknowledgements: I would like to thank fellow Addgenies Joanne Kamens, Marcy Patrick, Aliyah ...

  23. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...transformation efficiency of the strain: when asking the cell to perform multiple recombination events, all linear...linear fragments need to get into the same cell. For simple cloning requiring just one or two recombination...recombination events, homemade competent cells of 107 CFU/μg are perfectly fine, yet performing multiple modifications...modifications will require ultracompetent (109 CFU/μg) cells. Primer design Using specific primer design (Figure...

  24. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...using an electric charge to help the DNA enter the cells. Some samples require a reduced antibiotic concentration...concentration or need to be transformed into different cells than were originally requested. Most of our samples... Start by scanning the sample barcodes into the cells in the same order that they exist within the rack...storage process - from deposited DNA to transformed cells and finally to bacterial glycerol stocks that are...

  25. Bioinformatics at Addgene

    Type
    Blog Post
    ...designed to help us work in large formats, such as 96-well microplates, and thanks to our dedicated product...: Customizable to our specific needs Modern and well-supported, so we could focus on our development ... workflow would handle plasmid sequences on a 96-well plate basis, but for rapid testing and refinements...blog:  The Power Behind NGS Plasmid Validation: SeqwellHow to Keep a Lab Notebook for Bioinformatics AnalysesAAV...

  26. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ... be safely used to deliver a genetic payload to cells, and the complexity of effectively deploying them... can be to use. AAVs, which can target different cell types depending on their serotype, are particularly...amount of vectors available and number of requests tell only part of the story. Viral vectors are, after...experimental data in a standardized format, sharing how well a given viral vector worked — or didn’t work — in...

  27. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...named) “nanopores” that are attached to the flow cell membrane and have an electrical current running ...faster turnaround time than short-read sequencing, as well as the ability to sequence an entire viral genome..., with significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly McQueary References ...Addgene The Power Behind NGS Plasmid Validation: seqWell Addgene’s Tips for Plasmid Quality Control Additional...

  28. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...Many thanks to our guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of...by Future of Research Executive Director, Gary McDowell. On Dec 1st, the threshold at which salaried workers...come under this ruling, regardless of visa or fellowship status, in addition to certain staff scientists...

  29. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...other journals and several funding agencies (e.g. Wellcome Trust, NIH Data Sharing Policies, etc.) and is...numerous objections and concerns. Perhaps least compelling of these were those from researchers who wanted...released, we will be in danger of having to deliver on well-meaning but impractical requirements imposed by ...should open up new opportunities for research as well as increasing transparency. Thank you to our guest...

  30. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ... The loading control is supposed to control for, well, loading errors. When using it to normalize your...Most housekeeping proteins are highly expressed in cells. This means that at sample concentrations appropriate...assay. This is influenced by sample concentration as well as by antibody dilution — both factors we’ve covered... C, indicating that β-actin is less sensitive as well as less linear than the total protein quantification...

  31. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...academia Require that postdoc salaries be paid on fellowships instead of grants such that PIs no longer save...brought in editors from a variety of publications as well as young PIs and all gave advice on how to succeed...Rafael Luna, Joan Reede, Alberto Roca, and Jessica Tytell made it was obvious that more data is needed for...discluded/not treated equitably even when hired; well-meaning mentors often fail to judge the work of ...

  32. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...Every time I asked a question, I felt like I was yelling. I imagined 6 years of biting my tongue and gratefully...lab across the hall or in the same department as well. What do they have to say about this lab’s reputation...appointments with group members or are they frequently cancelled? Choosing a role model Make sure the mentors ...target up to fail, shifting goal posts without telling the target Warning signs while interviewing: Lab...

  33. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...mismatched) around the ligation junction in 16 wells of a 96-well plate, allowing 6 conditions to be screened... (2006): 687-699. PubMed PMID: 16464020.  3. Ellenberger, Tom, and Alan E. Tomkinson. "Eukaryotic DNA ...ligase bound to nicked DNA-adenylate." Molecular cell26.2 (2007): 257-271. PubMed PMID: 17466627. 25. Luo...

  34. The 12 Days of CRISPR

    Type
    Blog Post
    ...function using transcriptional recording sentinel cells! 🎵 On the 11th day of CRISPR, Addgene gave to me...Enhanced prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th ...activation and interference screens for primary human T cells🎵 On the 3rd day of CRISPR, Addgene gave to me: ...

  35. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    ...ImagingResearchers: Andrea Radtke, Ziv YanevCellosaurus: A Cell Line Information DatabaseResearcher: Amos...Undergraduate Research Experiences (CUREs)Author: Jason PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging...

  37. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...multiplex gene editing: Guest blogger Qichen Yuan tells us about a new tool for efficient multiplex base...Demethylation: Guest blogger Daniel M. Sapozhnikov tells us how to use dCas9 (with no modifications!) as ...research story, I couldn't resist adding in this excellent Q&A from Tim Miles on everything from AAV prep...

  38. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    ...next week - be sure to come by booth #851 and say hello! Just in time for this meeting, Addgene is launching...synthetic biology, gene cloning and expression, as well as guide pages for Synthetic Biology and Bacterial...of a useful plasmid that’s not in the collection? Tell us about it with our Suggest a Plasmid Form! Finally...

  39. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...Mammalian cells provide scientists with the means to study biological processes on the cellular level instead...with tissue culture The cultivation of mammalian cells in the lab, or tissue culture as it is commonly ...

  40. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ... Immune cells Flow cytometry (potentially others) Data repository From the Human Cell Differentiation...repository   HuBMAP Consortium Healthy human cells Imaging (IHC, ICC, IF, etc.)  Data repository...
Showing: 761 - 800 of 1029 results