We narrowed to 444 results for: ENA
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TypeBlog Post...cultures or plants, our transient expression method enables us to quickly screen and investigate transcription...
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Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...using/depositing. Our viral vectors data is less amenable to such analysis, due to its inherent bias: Addgene... -
Hot Plasmids Spring 2024
TypeBlog Post...PE7, it will be exciting to see future studies enabled by this tool as well as additional potential improvements... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog Post...spp., Alcaligenes spp., Aliivibrio salmonicida, Anabaena spp., Azospirillum braziliense, Azotobacter spp... -
CRISPR-mediated Plant Base Editors
TypeBlog Post...gene system to clone multiple protospacers which enables us to target more than one genomic site simultaneously... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post...restriction-modification systems mediate plasmid maintenance." Journal of bacteriology 177.12 (1995): 3451... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...PMC5424143. 2. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling of off-target cleavage by... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...amplification step in combination with the ssRNA reporter enables SHERLOCK to detect DNA or RNA with atomolar sensitivity... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...glycoprotein pseudotyping of lentiviral vectors enables retrograde axonal transport and access to the nervous... -
Plasmids 101: Gateway Cloning
TypeBlog Post... variety of plasmids. Speed The Gateway system enables the generation of the expression construct in only... -
5 Reasons to Use Reddit for Science Communication
TypeBlog Post...moderators for keeping the quality high and thus enabling a productive discourse. They even advised me on... -
CRISPR Activation: A Practical Guide
TypeBlog Post...Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H,... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post...that could be used to study a wide variety of phenomena, it’s possible that we’ll learn much more from... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...Majdoul, S., Martin, S., Merten, O.W., Galy, A., Fenard, D. ”Influence of mildly acidic pH conditions on... -
Tips for Using FRET in Your Experiments
TypeBlog Post...Indeed, FRET was one of the first techniques which enabled the measurement of single molecule interactions... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...nobody is really owning. Conflict in this latter scenario usually manifests as the assumption “I thought... -
Five Popular Model Organisms, Part 2
TypeBlog Post.... (2018). Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post...target. RNAi is a valuable tool for your research arsenal, and there is still a dedicated field of study ... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post... Transfecting these three plasmids into cells enables the production of lentiviral vectors that are competent... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier...