The Challenges of Cell Culture
Type
Blog Post
... of tracking how many passages the cells have undergone and when to throw them away and thaw a new batch...
We narrowed to 437 results for: ERG
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Plasmids 101: Stringent Regulation of Replication
TypeBlog Post...Grimwade et al., 2018). Rather than serving as an energy source, ATP binding is one more way for the cell... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...confluency but high enough to promote cell growth. Overgrown cultures stimulate quiescence, the resting phase... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog Post...of genome editing tools. Jieze Zhang is an undergraduate student studying biochemistry at the University... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...-McFarland, Lisa Gorski, Mark O. Martin, Maureen Berg, Michael D. L. Johnson, Tiffany Lowe-Power, Eric... -
How to Write a Scientific Review Article
TypeBlog Post...ecology, this can be represented by the tip of the iceberg. For writers, this is probably “a normal day.” ... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog Post...clinical laboratory. What can we do to stop the emergence of antibiotic resistance? The problem with this... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...the resident proteins within these compartments undergo significant post-translational modifications including... -
Plasmids 101: Control Plasmids
TypeBlog Post...Plasmid A is the decreased expression of Gene X. Ergo the positive control(s) should decrease the expression... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...likely won’t be long before other data repositories emerge that allow efficient searching and extraction of... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post..., 10 General expression mRNA Yeast adjacent, divergently transcribed promoters Inducible with galactose... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post... A. Khavari. "Research Techniques Made Simple: Emerging Methods to Elucidate Protein Interactions through... -
Plasmids 101: Biotinylation
TypeBlog Post...gluconeogenesis, lipogenesis, amino acid metabolism and energy transduction. Biotinylation is the general process... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...protein. This is clearly the case when proteins undergo post-translational modification with fatty acyl... -
Five Popular Model Organisms, Part 2
TypeBlog Post...blog post to see even more genetic systems for emerging model organisms!) Escherichia coli One of the ... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...-SspB heterodimerizes under blue light BcLOV4 undergoes a conformational change and binds the plasma membrane... -
The Advances Behind The World's First Personalized CRISPR Treatment
TypeBlog Post...even further. In the meantime, base editing will undergo even more improvements in the lab. We’re so happy... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post.... https://doi.org/10.1007/978-1-60761-657-3_10 Vergani-Junior, C. A., Tonon-Da-Silva, G., Inan, M. D.,... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog Post...delivered and expressed transiently using a standard detergent, Polyethylene Glycol (PEG), although the application... -
10 Steps to a Perfect Science Talk
TypeBlog Post...about the problems you are addressing by using an energetic tone of voice. Your goal is to be conversational...