We narrowed to 430 results for: ERG

1. The Challenges of Cell Culture

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   Blog Post

   ... of tracking how many passages the cells have undergone and when to throw them away and thaw a new batch...

2. Getting the Most from Your Lentiviral Transduction

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   Blog Post

   ...confluency but high enough to promote cell growth. Overgrown cultures stimulate quiescence, the resting phase...

3. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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   Blog Post

   ...the resident proteins within these compartments undergo significant post-translational modifications including...

4. Plasmids 101: Control Plasmids

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   Blog Post

   ...Plasmid A is the decreased expression of Gene X. Ergo the positive control(s) should decrease the expression...

5. Data Freedom: The Expansion of Data Sharing in Research Publications

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   Blog Post

   ...likely won’t be long before other data repositories emerge that allow efficient searching and extraction of...

6. Plasmids 101: The Promoter Region – Let's Go!

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   Blog Post

   ..., 10 General expression mRNA Yeast adjacent, divergently transcribed promoters Inducible with galactose...

7. Plasmids 101: Biotinylation

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   Blog Post

   ...gluconeogenesis, lipogenesis, amino acid metabolism and energy transduction. Biotinylation is the general process...

8. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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   Blog Post

   ... A. Khavari. "Research Techniques Made Simple: Emerging Methods to Elucidate Protein Interactions through...

9. Five Popular Model Organisms, Part 2

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   Blog Post

   ...blog post to see even more genetic systems for emerging model organisms!) Escherichia coli One of the ...

10. Plasmids 101: Expressing shRNAs for RNA Interference

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    Blog Post

    .... https://doi.org/10.1007/978-1-60761-657-3_10 Vergani-Junior, C. A., Tonon-Da-Silva, G., Inan, M. D.,...

11. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    Blog Post

    ...-SspB heterodimerizes under blue light BcLOV4 undergoes a conformational change and binds the plasma membrane...

12. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post

    ...protein. This is clearly the case when proteins undergo post-translational modification with fatty acyl...

13. Hot Plasmids: Summer 2024

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    Blog Post

    ...than using more complex protective methods or detergents like CHAPSO, while requiring a lower concentration...

14. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post

    ...21561674. PubMed Central PMCID: PMC3159719. 6. Firnberg, Elad, and Marc Ostermeier. “PFunkel: Efficient...

15. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    Blog Post

    ...delivered and expressed transiently using a standard detergent, Polyethylene Glycol (PEG), although the application...

16. 10 Steps to a Perfect Science Talk

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    Blog Post

    ...about the problems you are addressing by using an energetic tone of voice. Your goal is to be conversational...

17. Site Directed Mutagenesis by PCR

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    Blog Post

    ...site, digestion with the appropriate enzyme will merge two smaller bands visible in a digest of the unmodified...

18. Evolution of Lab Techniques

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    Blog Post

    ...at Harvard University currently studying the serotonergic system and behavior.     References 1. Southern...

19. How to Negotiate a Successful Lab Start Up Budget

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    Blog Post

    ...before hand, you’re saving the institute time and energy that would typically be wasted going through some...

20. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    Blog Post

    ...-1.0 expresses RFP when the construct has not undergone recombination. This default state was disproportionately...