...week within two months. That said, Ebola is not considered a “terribly infectious disease” – infection occurs...via body fluids not through the air and that’s considered unlikely to change – but the illness has been... critical for viral attachment and fusion. Once inside a host cell, new evidence in PLoS Pathogens shows...

...and on-targeting likely more efficient.  These insights are hard to appreciate without computational support...tools The goal of this post is to provide some insight into available CRISPR software tools, what problems... software tools that apply some approximate or sensible rules to determine the value of one sgRNA design...functions are not entirely necessary but should be considered extremely useful, depending on design goals. ...

...quoted as saying when the news first broke. "Considering that many human diseases are caused by genetic...presented the first detailed picture of Cas9's three-dimensional shape via x-ray crystallography, an advance they... and mainstream the news on CRISPR has gotten, consider these headlines in The New York Times and Forbes...

...right! He began to study the A. victoria gene responsible for encoding GFP, and, in 1992, he reported its...hypothesized that GFP-like proteins could be responsible and began studying these marine organisms to ... fell into place, as they, taken alongside the insights provided by the studies in Tsien lab, covered ...

...compiled our top career blog posts into one comprehensive, downloadable guide: the Addgene Science Career...you’d like to see discussed on the Addgene blog, consider writing a guest post. Additional resources on... Find job listings at Addgene Get more career insight from Addgenies ...

...number of building blocks with just two or three inexpensive enzymes. Now, a new toolkit - aptly named GreenGate...endonuclease of choice, BsaI, which can be very sensitive to temperature fluctuations. He says their lab...problem back to the endonuclease. “It’s just a sensitive protein,” he says. “There is nothing you can do...

... from other scientists doing interesting work. Consider this to be like any other research project. You...develop relationships, but if it is not possible consider a phone or video call. If you do decide to use...personal questions can elicit the most valuable insights you will get from the interaction. Most people...

...organism has already been made, and it would be expensive and time-consuming to repeat the process with ...spontaneous conversion did not occur. Tobacco suspension cell culture with cytosol-targeted GFP. White...level necessary to observe a signal. Red signal intensity increases with GFP concentration and saturates...

...partners, with the launch of antibodies in China and expansion of viral services available in South Korea.  ...addition, we released the MORF collection, a comprehensive transcription factor library, in lentiviral ..., Korea, Japan, India, and Brazil. Through an expansion of our export license application service, we’...

...a new and versatile binary Ti vector for both transient and stable gene expression applications in plants... (GUS) and GFP reporters, as well as multiple transient and transgenic systems, Sahoo et al. showed that... a novel versatile gene expression vector for transient assays as well as stable expression of foreign...

..., yellow, cyan, blue, and orange spectra. A comprehensive list of cell markers all tagged with mEmerald...multicolor labeling and FRET. Specialty constructs for biosensing. Over 300 plasmid backbones spanning a rainbow...color variants. The mEmerald plasmids offer a comprehensive list of readily available targets since the ...

...fluorescent proteins to observe what’s happening inside cells for at least a couple of decades. Green is... PA FPs with light of specific wavelength and intensity allows for optical labeling and tracking of proteins...capable of revealing, for example, how muscles work inside a living animal. For those making use of these ...

... these techniques and is a multi-step process consisting of digestion, purification, ligation, and transformation..., TOPO cloning, one step cloning, and overlap extension PCR. Although each type of cloning has its advantages...combine the products and digest with methylation sensitive restriction enzyme DpnI to remove the templates...

...for the user. This approach serves to maintain consistency with published screen interpretations and allows...curated CRISPR screen data are also available via comprehensive pre-compiled downloads and via a full-featured...are fully open access and available without any licensing restrictions.    As CRISPR technology continues...

...expanding your knowledge base extensively, you open doors to fresh insights and broaden your understanding...Making the Leap to Industry." He successfully transitioned to industry after his PhD and a couple of postdocs...

...put on typical middle age weight and “exercise” consisted of taking walks in the mall. I took action, joined...patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin ligand family... to raising funds and awareness of FOP. Please consider running with us or making a donation via the link...

...labs. Disposable plastic materials are fairly inexpensive in this country and we are quick to throw out... gel on a large glass slide using only surface tension.  It's a bit tricky to pour the right amount so...plastic and solder this thing together.  One of the downsides—there was no cover so you had to be careful not...

...the cell density and viability: Using a 5 mL pipette, transfer 0.5 mL of HEK293 cell suspension into a ...microcentrifuge tube. Vortex the cell suspension. Transfer 10 µL of cell suspension into the microfuge tube containing...Protocol Introduction Transfections allow for transient expression of a gene of interest in cell culture...culture. This protocol describes how to transfect suspension HEK293 cells with recombinant antibody plasmids...support reproducibility in science. General Considerations For antibody-expressing plasmids containing...antigen (EBNA). Safety Warnings HEK293 cells are considered biosafety level 2. Please ensure that you are...transfecting, seed a 108 mL culture of HEK293 cells at a density of 0.9 x 10 6 cells/mL in a 500 mL vented flask.Incubate...

...markers and replication origins, but there is extensive heterogeneity in the feature sequences due to ...control elements in commercial plasmids were inconsistent and sometimes clearly wrong. In an effort to...reliable feature annotations. Even with these extensive efforts, such an algorithm has limitations. For...

...a relatively pH-insensitive RFP variant, DsRed.T3, that’s connected to a pH-sensitive GFP variant, super...lysosome, and degradation of autophagic substrates inside the lysosome. Historically, methods for studying...