...human genome as a map with three coordinates: chromosome, start, and stop. For instance (chr3, 1, 10) ...stretch of DNA at the very beginning of the third chromosome, ten base pairs in length. An emerging family...coordinates alone aren’t very useful. We’ll need something like Google Maps to help us identify and visualize...700 terabytes per gram—and form the X-shaped chromosomes we all know and love. To understand compaction...

...updated protocols So that’s great, but what if someone in China improved upon that protocol? How would...frequently it's being updated now, but there are some classic protocols on Protocol Online that you might... As this is a growing market, I am sure I missed some of your favorite apps. If you know of a software...plasmids more successfully. And I’ll continue to add some of our basic protocols to the protocols.io app so...

...and expensive. In some cases, conjugation methods can interfere with binding, and some common antibody ...ELISA, advantages and disadvantages of each, and some considerations when using the method. ELISA basics...sample that is known to contain the target antigen. Some examples could be an endogenous sample of wild type...

...room names originated from. Let’s take a look at some of the stories behind the names. We also have many...insert specific pieces of DNA into vertebrate chromosomes via a cut and paste mechanism. Scientists reconstructed...couldn’t find the specific origins of the name but some of our Addgenies had an idea: sqh mutant fly ovaries...tour of Addgene’s new conference rooms and learned some fun facts about these genes and the origins of their...

...and hence making it searchable and shareable, but some ELNs provide better support for reproducibility ... use other tools in conjunction with the ELN, in some cases the integrations provide a direct boost to...storage resources remain live. This means that someone who wants to query and attempt to reproduce an ...access restrictions based on permissions, however, in some cases it would be possible to grant those wanting...

... low to nil base editing efficiency at some genomic sites. Some sites may be inaccessible to base editors...editors probably due to the presence of nucleosome or other proteins. You can detect base editing as early...editors, keep in mind that the generation of indel sometimes may also results in RE site destruction. Many...

...unmethylated sites in the chromosome. The more stable endonuclease can then cut the chromosome leading to cell ...improper plasmid partitioning, it will still inherit some toxin and antitoxin. However, the antitoxin will...-Kulka, and Gad Glaser. "An Escherichia coli chromosomal" addiction module" regulated by guanosine [corrected...

...to many complications, including the inability of some institutions to know exactly how many postdocs they...been able to gather data on changes to salary for some of the institutions that are meeting the salary ...length; that postdoc salaries will have to double at some institutions; and that institutions which can more...gradual adjustments towards this new level. Indeed some institutions did this. It doubtless took a lot of...

...-target DNA editing efficiency of APOBEC-Cas9 editisome complexes in real-time (St. Martin et al., 2019...transfected cells. C-to-T editing by the APOBEC-Cas9 editosome would restore eGFP fluorescence, making it easy...-target DNA editing efficiency of APOBEC-Cas9 editisome complexes. The eGFP reporters rely on the correction...reporters for single base editing by APOBEC-Cas9 editosome complexes. Scientific Reports 9: . https://doi.org...

...generated through measurement of events. An event is something that passes through the machine’s lasers and gives... read by the machine’s sensors. (Usually this something is a cell, but it could be debris or unbound antibodies...used to identify them, such as “CD123+CD11c- pDCs.” Some cells are defined not by the presence or absence...information FACS plots often contain other data. Some will have numbers by gated populations; this indicates...

...other than the cytoplasm. That is, unfortunately, some investigators realize too late that they have missed...secretory pathway, endocytic vesicles, mitochondria, lysosomes or they are secreted into the extracellular milieu...Carolina at Chapel Hill. Under the co-mentorship Blossom Damania and Jack Griffith, her research focuses...proteins in cellular organelles: serious pitfalls and some solutions." DNA and cell biology 32.11 (2013): 622...

...of year approaching. We’re always looking for something to brighten our day, whether it’s memories of ...finding new targets to measure! Let’s take a look at some of the hottest fluorescent biosensors new to Addgene...under two-photon illumination than previous GEVIs. Some constructs are also available as viral vectors (...pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 (AAV9) pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes of the...

...Morgan discovered that genes were found within chromosomes using the fruit fly far before we even knew that...Like human cells, yeast DNA is packaged into chromosomes and about 23% of yeast genes have a counterpart...al. "Efficient gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming...hartwell’s yeast: A model organism for studying somatic mutations and cancer." Nature Education 1.1 (2008...

...is only synthesized by plants, most bacteria and some fungi, thus mammals must obtain the vitamin through...diet was being supplemented with biotin. Thus something in the egg white was sequestering biotin from ...applications for biotin and avidin. A short list of some other common applications and links to relevant ...(McInnes et al., 1990). Photobiotinylation is sometimes easier and less expensive than other enzymatic...

...testable hypothesis - an educated guess about how some part of the world functions. Experiments come next...highlights data supporting unwanted results (14). Some loss of trust, I fear, has already come to pass....about it. And yet research shows that thoughts on some non-political issues are split along party lines...scientists to be more involved in their communities (something already encouraged by “Broader Impacts” requirements...

...cost with restriction mapping (Figure 1). Though some labs may prefer to send DNA directly for sequencing...sequencing machines (9). Today, similar technologies, sometimes referred to as sequence DNA as it synthesized ...laboratories. As scientific technologies rapidly progress, some common lab techniques we use now may soon become...3. Hoebeeck, Jasmien, Frank Speleman, and Jo Vandesompele. "Real-time quantitative PCR as an alternative...

...that you've made a conscious effort to eliminate some of the excessive costs in your lab. That in itself...focus on your research goals and hopefully remove some of the anxiety that comes with negotiations. This...their success. Therefore, assume positive intent. Sometimes, “no we don’t have the funds,” really means they...energy that would typically be wasted going through some of the questions we mentioned previously.  There...

... image from Evan Amos.   After the transfer, some areas of the membrane remain unbound by protein....antibodies and labels being used and may require some trial and error (Yang et al., 2012).  Choosing your...expressed protein at physiologically relevant levels. Some vendors will also test their antibody in a variety...polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy...

...is out of your hands, it’s helpful to focus on something controllable, like experiments (and their controls...secondary antibodies will be highly specific, but in some cases they may cross-react and bind to more than...secondary antibodies.   Application-specific controls Some antibody-based applications have very specific controls...rigorous experiments. While this blog touched upon some of the more critical controls for antibody-based...

... your experiments? In this post, we will discuss some broad classes of optogenetic tools, and what factors...in using optogenetic tools in their experiments. Some tools turn on and off rapidly with light to control...light source used.   Channel and activation kinetics Some channels turn on and off very rapidly in response...mechanisms that are often cell-type specific. If it is something you’re considering for your experiment, note that...