We narrowed to 869 results for: SOM

...cost with restriction mapping (Figure 1). Though some labs may prefer to send DNA directly for sequencing...sequencing machines (9). Today, similar technologies, sometimes referred to as sequence DNA as it synthesized ...laboratories. As scientific technologies rapidly progress, some common lab techniques we use now may soon become...3. Hoebeeck, Jasmien, Frank Speleman, and Jo Vandesompele. "Real-time quantitative PCR as an alternative...

...that you've made a conscious effort to eliminate some of the excessive costs in your lab. That in itself...focus on your research goals and hopefully remove some of the anxiety that comes with negotiations. This...their success. Therefore, assume positive intent. Sometimes, “no we don’t have the funds,” really means they...energy that would typically be wasted going through some of the questions we mentioned previously.  There...

...is out of your hands, it’s helpful to focus on something controllable, like experiments (and their controls...secondary antibodies will be highly specific, but in some cases they may cross-react and bind to more than...secondary antibodies.   Application-specific controls Some antibody-based applications have very specific controls...rigorous experiments. While this blog touched upon some of the more critical controls for antibody-based...

... your experiments? In this post, we will discuss some broad classes of optogenetic tools, and what factors...in using optogenetic tools in their experiments. Some tools turn on and off rapidly with light to control...light source used.   Channel and activation kinetics Some channels turn on and off very rapidly in response...mechanisms that are often cell-type specific. If it is something you’re considering for your experiment, note that...

...(Issa et al., 2023). This can be particularly burdensome in the brain where many different cell types,...License. Within the nervous system, remember that some serotypes can be transported retrogradely and/or...expression can still occur (Fig. 3) - for example, some neurons can transiently express TH - so you’ll need...control the timing of expression in your experiment. Some inducible constructs can exhibit leaky expression...

... image from Evan Amos.   After the transfer, some areas of the membrane remain unbound by protein....antibodies and labels being used and may require some trial and error (Yang et al., 2012).  Choosing your...expressed protein at physiologically relevant levels. Some vendors will also test their antibody in a variety...polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy...

... a forward or reverse orientation so you’ll need some way to verify that the insert ended up in the direction... plasmid) and plasmid backbone. Because you lose some DNA during the gel purification step, it is important...different migration speeds; after running the gel for some duration of time, these differently sized pieces...post or pre run in the table above, respectively). Some of the above stains require you to visualize your...

...Rocío López-Diego, & Zafira Castano Corsino. When someone says “internationalization” young hearts often ...talented professionals (who migrated from their country some years ago looking for their career improvement) ...with mentors who are working in USA and Europe, something that I would not able to do without a program ...Spanish scientists (MD, PhD and MD/PhD) established in some of the top Universities in USA and Europe (Harvard...

...into primary cells, this blog article will give you some tips to plan your experiment using the lentiviral...cells often relied on chemical (CaCl2, cationic liposome) or electrical (electric current forming membrane...and the marker can be separated by an internal ribosome entry site (IRES) and expressed under the same...supernatant in order to precipitate the viral particles. Some protocols suggest adding sucrose to the supernatant...

...Considerations Mismatch cleavage assay Knockouts Some point mutations Some insertions/deletions Very inefficient ...genome relies on proper gRNA design. While there are some cases where it makes sense to manually design a ...See Addgene's HDR blog post for more details. In some cases, there may be no PAM site close to your desired...lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. Both lentiviral...using cationic lipids, electroporation, or an endosomal approach. Similar to the mRNA method described... genotypes within the resulting cell population. Some cells may remain wild type due to either (1) a lack...Before choosing a validation method, ask yourself some questions: Does the resulting population need to...

...to as “Epi” illumination. Therefore these are sometimes call Epi-fluorescence microscopes. (See left image...cell cultures, small model embryos >15 μm Thsee are some of the most challenging samples to image as they...image thick scattering samples in their entirety, something that is not possible via traditional confocal ...and cleared tissues These samples always require some form of optical sectioning. Point scanning confocal...

...antibodies can be produced for research purposes, some of which take advantage of natural immune system...of the antibody. Let’s dive in and take a look at some of these applications. Western blot Western blots...Enzyme-linked Immunosorbent Assay). Immunoprecipitation Sometimes, instead of just detecting the presence of protein... are referred to as functional grade antibodies. Some of the research and therapeutic uses overlap: antibodies...

... the technology. In this blog, we will highlight some common MoClo applications and kits. Keep in mind...range of parts, including copy number machinery, chromosomal integration modules, and many more of the usual...conditional gene expression, multiplexed CRISPR, and chromosomal integration of larger expression cassettes (Shaw...addition to additional elements for S. cerevisiae chromosomal integration and gene expression, the system includes...

...knockouts using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR...also like to see what you think so we’ll ask you some questions on Friday - share with us how you use ...CRISPR guide! If you’re not into Twitter, we have some exciting news for you too! The CRISPR field is always...

... with Addgene mugs and some delicious hot chocolate for the lab. Here are some of our 2017 contest winners...but our community of creative scientists delivered some spectacular photos once again this year! All winners...

...along the way to ourselves so we decided to share some of our tips in our new series: Addgene Lab Tips!...tip #1: Glove trick Putting on lab gloves can sometimes be annoying. To make it easier, try this quick... to keep up to date on the latest episode.  Have some suggestions for future tips?  Send your ideas to...

...advantage of repair pathways within the cell — in some cases, the same repair pathways that were considered...Surprisingly, the team noticed that this editor sometimes introduced C → G edits at certain positions within...multi-step cellular repair pathway that is known for sometimes causing double-strand breaks (DSBs). The Fei-Long...common intermediate of CGBE activity, and they are sometimes processed by error-prone non-homologous end joining...

...research done in our depositor labs. Some are written by Addgenies and some are written by the depositors themselves...favorite posts from 2022! Wow! Our depositors do some really incredible science - I hope you've enjoyed...

...the loading and unloading of the column and give some tips for performing this purification.  Check out...titration by qPCR using SYBR green technology Here’s some advice on how to successfully set up your qPCR to... new to tissue culture, check out this video for some tips and best practices for getting started with...

...these plasmid deposits came in this year, there were some plasmids deposited as early as 2006 that have helped...your science along? We’ll also take a look back on some fascinating Addgene history that you might not have... into 2021. Check out the infographic below for some Addgene depositor fun facts for a quick way to grasp...