We narrowed to 421 results for: SUA

1. Tips for Getting a Faculty Position

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   Blog Post

   ...talk, simple in principle The first question I usually get about chalk talks is, what is a Chalk Talk?...

2. Transferable Skills Guide: Conflict Resolution

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   Blog Post

   ...really owning. Conflict in this latter scenario usually manifests as the assumption “I thought that was...

3. Quick Guide to Near-Infrared Fluorescent Proteins

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   Blog Post

   ... that produce enough light intensities in NIR. Usually, mercury lamps are suboptimal, metal halide lamps...

4. Antibodies 101: Single Chain Fragment Variables (scFvs)

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   Blog Post

   ...connected by a flexible linker peptide that is usually 15-20 amino acids long and made up of glycine and...

5. Filming Science Videos in the Age of Social Distancing

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   Blog Post

   ...scenes footage in the last several years, you can usually see a director sitting in their chair not watching...

6. Using Phosphoserine to Study Protein Phosphorylation

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   Blog Post

   ...ability to isolate purified phosphorylated products usually depends on tools such as phosphospecific antibodies...

7. Evolution of Lab Techniques

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   Blog Post

   ...sample thereby labeling it. The label can then be visualized by autoradiography and X-ray film for radioactively...

8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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   Blog Post

   ...common infections and opportunistic infections not usually seen in healthy individuals. Antiretroviral therapy...

9. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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   Blog Post

   ...reaction is typically fairly high, so there is usually no need to screen a large amount of colonies. Excluding...

10. Hot Plasmids: Spring 2025

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    Blog Post

    ...loose-seal, cell-attached recordings in primary visual cortex (V1) layer 2/3 (L2/3). B) Fluorescence from...

11. CRISPR 101: Off-Target Effects

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    Blog Post

    ...pathway, a growth factor, etc.) this control is usually not very meaningful. Unfortunately, there really...

12. A Control for All Seasons

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    Blog Post

    ...Samples are incubated with ‌secondary antibody as usual and the signal measured. If a nonspecific signal...

13. Viral Vectors 101: Optogenetic Tools

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    Blog Post

    ...it (Nagel et al., 2003). Inhibitory opsins are usually light-driven pumps that pass ions (Cl-, H+, Na+...

14. Antibodies 101: Multiplex Immunofluorescence

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    Blog Post

    ...to describe or classify the labeled structures visually; for example, confirming that your target protein...

15. Technique: Probe Phage Genomes for Host Binding Proteins

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    Blog Post

    ...library of transformants for single colonies (this usually requires 10-20 plates) (see box below). This initial...

16. Generating Mouse Models Using CRISPR/Cas9

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    Blog Post

    ...oligonucleotide, we aim to generate 50 to 100 mice and are usually successful in deriving mice carrying the intended...

17. Finding Your Perfect Job After University

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    Blog Post

    ...not suit everyone. My career path may seem quite unusual but it has allowed me to try out working in many...

18. Protocol - pLKO.1 – TRC Cloning Vector

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    Protocol

    ...in a sterile microcentrifuge tube. TIP: When visualizing DNA fragments to be used for ligation, use only...

19. A History of Genome Engineering in Popular Culture

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    Blog Post

    ...  Genetic Engineering Era: Pre-CRISPR  Having visualized and understood the DNA, the next milestone for...

20. Quick Guide to All Things Lentivirus

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    Blog Post

    ... will first need to transfect producing cells, usually 293T cells, with your transfer plasmid and your...