We narrowed to 424 results for: SUA

1. Lentiviral Vector Guide

   Type

   Guide

   ... Plasmid Interchangeable; usually encodes for VSV-G Interchangeable; usually encodes for VSV-G Safety ...Envelope plasmid — contains packaging gene env (usually VSV-G due to wide infectivity) VSV-G (vesicular...

2. The Fluorescent Vegetables in Aptamer Soup

   Type

   Blog Post

   ... K., et al. "A fluorescent split aptamer for visualizing RNA–RNA assembly in vivo." ACS synthetic biology...

3. Transferable Skills Guide: Conflict Resolution

   Type

   Blog Post

   ...really owning. Conflict in this latter scenario usually manifests as the assumption “I thought that was...

4. Quick Guide to Near-Infrared Fluorescent Proteins

   Type

   Blog Post

   ... that produce enough light intensities in NIR. Usually, mercury lamps are suboptimal, metal halide lamps...

5. Antibodies 101: Single Chain Fragment Variables (scFvs)

   Type

   Blog Post

   ...connected by a flexible linker peptide that is usually 15-20 amino acids long and made up of glycine and...

6. New Optogenetic Tools for Cytoskeleton and Membrane Control

   Type

   Blog Post

   ...oligomers that trap the fusion protein away from its usual location, preventing it from carrying out its proper...

7. Tips for Getting a Faculty Position

   Type

   Blog Post

   ...talk, simple in principle The first question I usually get about chalk talks is, what is a Chalk Talk?...

8. Antibodies 101: Introduction to Gating in Flow Cytometry

   Type

   Blog Post

   ...and how you can combine two colors (stains) to visualize four populations at once.   Paul Heisig is a Research...

9. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

   Type

   Blog Post

   ...cells, the viral life cycle initially proceeds as usual. Reverse transcription of the transgene occurs in...

10. Filming Science Videos in the Age of Social Distancing

    Type

    Blog Post

    ...scenes footage in the last several years, you can usually see a director sitting in their chair not watching...

11. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    ...ability to isolate purified phosphorylated products usually depends on tools such as phosphospecific antibodies...

12. Evolution of Lab Techniques

    Type

    Blog Post

    ...sample thereby labeling it. The label can then be visualized by autoradiography and X-ray film for radioactively...

13. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    ...common infections and opportunistic infections not usually seen in healthy individuals. Antiretroviral therapy...

14. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    ...reaction is typically fairly high, so there is usually no need to screen a large amount of colonies. Excluding...

15. Protocol - pLKO.1 – TRC Cloning Vector

    Type

    Protocol

    ...in a sterile microcentrifuge tube. TIP: When visualizing DNA fragments to be used for ligation, use only...

16. CRISPR 101: Off-Target Effects

    Type

    Blog Post

    ...pathway, a growth factor, etc.) this control is usually not very meaningful. Unfortunately, there really...

17. A Control for All Seasons

    Type

    Blog Post

    ...Samples are incubated with ‌secondary antibody as usual and the signal measured. If a nonspecific signal...

18. Viral Vectors 101: Optogenetic Tools

    Type

    Blog Post

    ...it (Nagel et al., 2003). Inhibitory opsins are usually light-driven pumps that pass ions (Cl-, H+, Na+...

19. Hot Plasmids: Spring 2025

    Type

    Blog Post

    ...loose-seal, cell-attached recordings in primary visual cortex (V1) layer 2/3 (L2/3). B) Fluorescence from...

20. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    ...to describe or classify the labeled structures visually; for example, confirming that your target protein...