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We narrowed to 410 results for: SUA

Showing: 381 - 400 of 410 results

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The Fluorescent Vegetables in Aptamer Soup

Type

Blog Post

... K., et al. "A fluorescent split aptamer for visualizing RNA–RNA assembly in vivo." ACS synthetic biology...
Transferable Skills Guide: Conflict Resolution

Type

Blog Post

...really owning. Conflict in this latter scenario usually manifests as the assumption “I thought that was...
Using Phosphoserine to Study Protein Phosphorylation

Type

Blog Post

...ability to isolate purified phosphorylated products usually depends on tools such as phosphospecific antibodies...
Evolution of Lab Techniques

Type

Blog Post

...sample thereby labeling it. The label can then be visualized by autoradiography and X-ray film for radioactively...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

...common infections and opportunistic infections not usually seen in healthy individuals. Antiretroviral therapy...
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Type

Blog Post

...reaction is typically fairly high, so there is usually no need to screen a large amount of colonies. Excluding...
CRISPR 101: Off-Target Effects

Type

Blog Post

...pathway, a growth factor, etc.) this control is usually not very meaningful. Unfortunately, there really...
A Control for All Seasons

Type

Blog Post

...Samples are incubated with ‌secondary antibody as usual and the signal measured. If a nonspecific signal...
Viral Vectors 101: Optogenetic Tools

Type

Blog Post

...it (Nagel et al., 2003). Inhibitory opsins are usually light-driven pumps that pass ions (Cl-, H+, Na+...
Antibodies 101: Multiplex Immunofluorescence

Type

Blog Post

...to describe or classify the labeled structures visually; for example, confirming that your target protein...
Technique: Probe Phage Genomes for Host Binding Proteins

Type

Blog Post

...library of transformants for single colonies (this usually requires 10-20 plates) (see box below). This initial...
Generating Mouse Models Using CRISPR/Cas9

Type

Blog Post

...oligonucleotide, we aim to generate 50 to 100 mice and are usually successful in deriving mice carrying the intended...
Finding Your Perfect Job After University

Type

Blog Post

...not suit everyone. My career path may seem quite unusual but it has allowed me to try out working in many...
Quick Guide to All Things Lentivirus

Type

Blog Post

... will first need to transfect producing cells, usually 293T cells, with your transfer plasmid and your...
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

Type

Blog Post

... digestion products (which could be easier to visualize and identify). If you need/want to put the digestion...
When Fidelity Matters: A frank discussion about ligase fidelity

Type

Blog Post

...differences. The original creators of this technique visualized the ligation product in a gel, but we can now...
A Guide to Getting Started in Undergrad Research

Type

Blog Post

...and responsibilities can vary "Lab assistants" usually wash glassware, prep media, etc. "Research assistants...
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

Type

Blog Post

...to regulate gene expression (CRISPRi/CRISPRa), visualize specific genomic loci (CRISPR-imaging), or to ...
A History of Genome Engineering in Popular Culture

Type

Blog Post

... Genetic Engineering Era: Pre-CRISPR Having visualized and understood the DNA, the next milestone for...
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

Type

Blog Post

...to cell-type specific promoters; however, those usually drive weak expression of downstream genes. Strong...

Showing: 381 - 400 of 410 results

Results per page:

The Fluorescent Vegetables in Aptamer Soup

Transferable Skills Guide: Conflict Resolution

Using Phosphoserine to Study Protein Phosphorylation

Evolution of Lab Techniques

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

CRISPR 101: Off-Target Effects

A Control for All Seasons

Viral Vectors 101: Optogenetic Tools

Antibodies 101: Multiplex Immunofluorescence

Technique: Probe Phage Genomes for Host Binding Proteins

Generating Mouse Models Using CRISPR/Cas9

Finding Your Perfect Job After University

Quick Guide to All Things Lentivirus

Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

When Fidelity Matters: A frank discussion about ligase fidelity

A Guide to Getting Started in Undergrad Research

An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

A History of Genome Engineering in Popular Culture

FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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