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We narrowed to 672 results for: abo.1

Showing: 381 - 400 of 672 results

  1. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    ...with Crystal Shih Podcast breakdown Intro Chapter 1: Who is Crystal Shih? Crystal's educational background...therapeutics at Novartis - listen to learn more about Crystal’s exciting career path, to gain insight ...Chapter 2: What's a lab group in industry? The collaborative nature of working in industry and how delegating...metrics for success. The importance of thinking about the big picture and not just the next experiment...resources in our Mentoring for Scientists Series Learn about Turning Your PhD into a Career with Joanne Kamens...

  2. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...for example, split cells on a Monday diluting them 1:10, you should be able to split on the following Thursday...Immunology. You can find Sana on LinkedIn.   References 1. Yin, R., et al. "Gene expression profiling analysis...of the hood while work is in progress.Learn more about cell culture contamination in this post from the... bath constantly at 37°C for this purpose.Learn about the effects of CO2 on sample pH Cell morphology:... Set up a routine to split cells Don’t be lazy about splitting cells, instead try to form a routine. ...secretion of lactic acid (a by-product of cellular metabolism that is toxic to the cells), and need to be split...

  4. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ...editors (ABE) mediate A•T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes...dCas to the genomic DNA of interest  Figure 1. When ABEs are in action they bind DNA so that the...more blog posts about base editing Learn about another evolved base editor: ABE8e Read about xCas9, a CRISPR...

  5. Of Myc and Men

    Type
    Blog Post
    ...more applications.   Fig. 1 Making a Protein Tag (Abridged) (1) Locate a promising monoclonal antibody...Do you ever wonder about the origins of some of the common techniques or tools you use in the lab? Take...times of the myc tag.  Origins of protein tags For about as long as the disciplines have existed, cell and...examples of new versions of Myc1-9E10 mentioned above basically involve taking the Myc1-9E10 variable ...

  6. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...Updated Jun 1, 2021 by Meghan Rego. Reproducible data are key to science, so scientists are used to repeating...the DNA packaged inside the viral particles (Figure 1). Briefly, packaged DNA is extracted from purified...viral genome sequencing (VGS). Why should you care about viral quality control? AAV vectors are produced ... let us know if you have any specific questions about viral DNA NGS you’d like us to discuss. We are also...or at [email protected] to answer your questions about our quality control process! The images in this...

  7. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...cleavage (Harrington et al., 2017). Figure 1: Anti-CRISPR proteins (Acr's) can block CRISPR activity... ways to anti-CRISPR proteins in your experiments 1. Reduce off-target effects Prolonged Cas activity ...Bondy-Denomy et al., 2018). For example, AcrIIA4 mentioned above indicates that it’s the fourth Acr protein discovered...Cas9 Variant for Your Next Experiment Read more about Optogenetic Control of CRISPR   Resources on Addgene.org...

  8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...fluorophores are constantly being engineered. Table 1 lists a few common fluorescent proteins and their ...definitions found in Table 2 may not apply. Table 1: The specific mutations comprising common fluorophores... activity, and much more. Read on to learn more about GFP, how scientists have evolved this versatile ...effects of alterations to this activity. Learn more about optogenetics at OpenOptogenetics and find optogeneic...

  9. Promoters

    Type
    Guide
    ...Strong mammalian promoter from human elongation factor 1 alpha CAG Constitutive Strong hybrid mammalian promoter...of interest is expressed. Summary Promoters are about 100-1000 base pairs long and are adjacent and typically...Inducible by arabinose Promoter of the arabinose metabolic operon trp Repressible by tryptophan Promoter ...

  10. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...existing protein structures, called Top7. Figure 1: Top7 receives the Nobel Prize. Created with BioRender.com...proteins also helped researchers understand more about the biophysics of protein folding and selective ...

  11. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...be selected for these small ligands. References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of...Biological Applications.” Sensors (Basel). 2012; 12(1): 612–631. PubMed PMID: 22368488. PubMed Central PMCID...target metabolite creates an aptamer that will only bind to DFHBI in the presence of that metabolite (Fig...approach less sensitive in most applications. Learn About Fluorescent Biosensors Light-up aptamers (or, fluorescing...fluorophore protein. Years later, Samie Jaffrey’s laboratory took advantage of their deep knowledge of the...Tips for Using FRET in Your Experiments Learn more about GFP Controlling Protein Activity with GFP Resources...

  12. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ... Hot plasmids covered in this segment Hot Plasmid 1: CRISPR X from Michael Bassik's Lab - A new tool for...this new way to learn about plasmid technologies. If you have any comments about the Hot Plasmids Podcast...with these efforts, you’ll find new ways to learn about science careers, lab protocols, and, of course, ...each one, plug in some headphones to learn more about the latest and greatest plasmids through this podcast...

  13. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ... necessary for advancing the field. References: 1. Internal initiation of translation of eukaryotic ... of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice. Kim et al...genes above with your gene or genes of interest. Some of the plasmids listed in the table above (and their... an internal region of the mRNA. In the figure above, you can see that the IRES element acts as another... first discovered in picornaviruses, are short (about 20 amino acids) and produce equimolar levels of ...

  14. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...true reproducibility. The following are critical: (1) connectivity to other data sources used in research...engagement with the Wisconsin research community were to: (1) prevent vendor lock in by making it easy to get data...RSpace is integrated with most of the tools noted above, and has a modern API. Integrations with additional...into RSpace. The kind of informal conversations about research that can take place in Slack normally never...RSpace documents to external data sources (described above) further enhances the value the repository integrations...Minisymposium on Reproducibility Read all blog posts about scientific sharing Resources on Addgene.org Find...

  15. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...passionate about health equity, science communication, and public health.     References 1. O'Neill, J... to the development of resistance include (Figure 1): Over-prescription of antibiotics (for example, ...resistant infection or not, because scientists in the laboratory will need to isolate and grow whatever bacteria...method that is frequently used in the clinical laboratory. What can we do to stop the emergence of antibiotic...CDC. Antibiotic resistance can be scary to think about, but we can all do our part to learn all we can,...

  16. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ... cerevisiae (Tuller et. al., 2010). Figure 1: An overview of peptide synthesis. A ribosome interacts...McClain WH (2000) The G·U wobble base pair. EMBO Rep 1:18–23 . https://doi.org/10.1093/embo-reports/kvd001...usage bias Read more about plasmids in our Plasmids 101 blog series Learn more about trends in synthetic... out Addgene's Molecular Biology Reference Read about genetic code expansion Browse our plasmid collection...

  17. Hot Plasmids - August 2020

    Type
    Blog Post
    ...membrane targetin sequence derived either from p63(1-29) and Lck. Image from Chertkova et al., 2020. ...Reports. 2020. https://doi.org/10.1038/s41598-020-57500-1 The CRISPR corner By Jennifer Tsang Here are a few... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's... a cell. The Goedhart lab, Gadella lab, and collaborators have an ongoing effort to improve genetically..., that are optimized for excitation wavelengths above 1,000 nm and enable two-color imaging with red fluorescence...

  18. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ... medicinal plant, the Catharanthus roseus (Figure 1). The ultimate goal of our research is to enhance ...activities in seed plants." Plant cell reports 9.1 (1990): 1-5. PubMed PMID: 24226366. Lee, Min Woo, and Yinong...Generating a transgenic plant takes even longer, is more labor intensive, inefficient, and often not reproducible... region is transferred into the plant. In the laboratory we can utilize this natural process by replacing...system. The vectors mentioned in this blog post above are ready to use vectors, but with the MoClo system...

  19. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...Spaghetti Monster probes at Addgene Uses of smFPs 1. Multi-channel connectomic tracers Fluorescent proteins... expanded use of smFPs in the future. References 1. Viswanathan, Sarada, et al. "High-performance probes...enable accurate labeling of neurons. As discussed above, weakly expressed proteins in particular are difficult...resembles images of the deity (see comparison image above). The church of the Flying Spaghetti Monster is ...tags. 4. High-resolutions microscopy As indicated above, smFPs can be used with conventional microscopes...

  20. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...depths (1 mm), important for certain applications such as in vivo tissue imaging.   Figure 1: Ace-mScarlet...especially essential. If you want to learn more about how viruses can be used for chemigenetics, biosensors...
Showing: 381 - 400 of 672 results