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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 535 results for: genome

Showing: 381 - 400 of 535 results

  1. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...Protein-DNA Interactions CRISPRainbow and Genome Visualization CRISPR Between the Genes: How to Experiment...methods and tools for studying chromatin and the epigenome. If you have been considering some new techniques...Regulation CRISPR 101: Epigenetics and Editing the Epigenome CUT&RUN: An Improved Method for Studying Protein-DNA...Experiment with Enhancers and Epigenomics I would like to thank the many scientists with me at the Keystone ...

  4. Clear and Accessible Writing

    Type
    Blog Post
    ...are captured at the 17%, 25%, and 60% layers, and genome-containing virions are captured at the 40% layer...

  8. Working with Nuclear Receptors

    Type
    Blog Post
    ...receptors and signaling cascades conceivably allows the genome to sense the impact of the total environment on...

  9. Browse

    Type
    Collection
    ...Collections Structural Genomics Consortium Plasmids Browse All SGC Plasmids Structural Genomics Consortium Plasmids...Browse plasmids from the Structural Genomics Consortium (SGC) for 3D protein structure studies Plasmid...

  10. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector for mammalian genome editing Tet-pLKO-puro...Fusion Protein Function: Viral Vector Delivery, Genome Modification, mRNA Regulation, and More Selectable...can also be used for for viral vector delivery, genome modification, reporter assays, shRNA expression... efficiency, but do NOT integrate into the host genome Addgene has an extensive collection of plasmids...nondividing cells, integrate into host genome pLenti CMV GFP DEST - Lentiviral Gateway destination...Retroviral Easy and safe to use, integrate into host genome MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian...efficiency, but do NOT integrate into host genome pAdTrack-CMV - Shuttle vector for...

  11. Microbiology Resources

    Type
    Collection
    ...bacterial genome editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome Engineering...using CRISPR in your lab, specific CRISPR tools for genome editing in bacteria, and more. Synthetic Biology...range of organisms. Find CRISPR tools for use in genome editing of bacteria. CRISPathBrick Multiplex Plasmid...opens in a new window) JGI Integrated Microbial Genomes and Microbiomes (IMG/M) (Link opens in a new window...Program (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window) Return...

  12. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...Plasmid Collections Structural Genomics Consortium Plasmids Structural Genomics Consortium Plasmids You may...Plasmids from the Structural Genomics Consortium (SCG), which are primarily used for determining protein...like... David Waugh Lab plasmids The Structural Genomics Consortium is a not-for-profit organization that...

  13. Viral Production

    Type
    Collection
    ...sequencing of the Viral Genome Next-generation sequencing is performed on viral genomes isolated from the final...confirm the identity and integrity of the viral genome and the absence of unexpected DNA contaminants....pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] ...Electron Microscopy The ratio of empty to full (i.e., genome containing) AAV particles within representative...of a lentiviral vector, incubated for 72 h, and genomic DNA is extracted. Primers and probes targeting ...harvesting cells several days later, and isolating genomic DNA. This DNA is then used as a template to amplify...

  14. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...exactly match your genomic target? Variation between a given gRNA sequence and your genomic target may reduce...Plasmids: gRNAs Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...gRNA plasmids designed to target various genes or genomic regions. It is highly recommended that you determine...

  15. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...recombinase systems, how to use them to modify the genome or control gene expression, and find plasmids for...derived from fungi and bacteriophages are powerful genome engineering tools used to precisely manipulate ... payload containing an attB target site into a genomic attP site. So far, their practical application ...site-specific recombinases can be used to modify the genome or control gene expression or skip ahead to browse... Vectors . Flp-IN: A single FRT site within the genome of a host cell line serves as the target location...stable integration of the gene of interest into the genome at the defined FRT site. Multiplexing: Orthogonal... may cause repetitive recombination leading to genomic instability and cell death. It is important to ...

  16. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ... Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...the nickase to the target site by homology to a genomic DNA locus and encodes a primer binding site and...Cas9 (dCas9) can be used to purify a region of genomic DNA and its associated proteins, RNA, and DNA. ...fluorescent protein (FP) can help visualize specific genomic loci using fluorescent microscopy in living cells...sequence to direct the dCas9-FP fusion to a specific genomic sequence - potential target locations can include...
Showing: 381 - 400 of 535 results