Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 949 results for: org

Showing: 381 - 400 of 949 results

  1. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...Nonetheless, the initial release caused a storm as the organization left open the question of how much data was ...to make the underlying data easily available, organized in a form that can be understood by others, balanced...each individual video. To PLOS’s credit, the organization subsequently clarified what the policy intent...researchers. Yet accurate and comprehensible organization of data is essential for efficient science. ...

  2. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...your host organism. Because transcription machinery differs between cell types or organisms, promoters...GFP Resources on Addgene.org Find Plasmids for Your Research     ...  Kendall Morgan...

  3. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016/j.jneumeth.2008.05.003 Ghosh, R., Gilda... Review of Proteomics, 11(5), 549–560. https://doi.org/10.1586/14789450.2014.939635 Gilda, J. E., & Gomes...Analytical Biochemistry, 440(2), 186–188. https://doi.org/10.1016/j.ab.2013.05.027 Additional Resources on... and Optimizing a Western Blot Resources on Addgene.org Addgene's Western Blot Protocol  ...

  4. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...functional, fluorescent protein in almost all organisms and cell types, (ii) no need to add a co-factor...protein are available (Cranfill et al., 2016 and FPbase.org). A number of variants remain fluorescent under...mTurquoise2, not shown) remains fluorescent in acidic organelles (Figure 1), showing that its acid tolerance is...Fluorescent Proteins 101 eBook Resources on Addgene.org Browse the Fluorescent Protein Collection Find...

  5. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...Guest blogger Todd Waldman, Professor at Georgetown University, contributed to this post. Adeno-associated...recombination pathway. The Waldman lab at the Georgetown University School of Medicine has used this gene... at the Lombardi Comprehensive Cancer Center, Georgetown School of Medicine. His research currently focuses...Made in Heaven: CRISPR and AAV Resources on Addgene.org Check out our Viral Service Find AAV Vectors...

  6. March for Science

    Type
    Blog Post
    ...rights (2). Less formally, governments are the organizations that make and enforce the rules of the lands...climate change, the safety of genetically modified organisms (GMOs), the absence of a link between vaccines... while their website lists over 200 partner organizations, and 514 satellite marches are scheduled to ...Chronicle of Higher Education: “March for Science” Organizer Says It’s About the Public, Not the Scientists...

  7. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...Research Volume 42, Issue 22, Page e168. https://doi.org/10.1093/nar/gku936 Eva K. Brinkman, Arne N. Kousholt...Research Volume 46, Issue 10, Page e58. https://doi.org/10.1093/nar/gky164 Cancellieri S, Canver MC, Bombieri...editing. (2019) Bioinformatics 36:2001–2008. https://doi.org/10.1093/bioinformatics/btz867 Haeussler M, Schönig...selection tool CRISPOR. (2016) Genome Biol 17: https://doi.org/10.1186/s13059-016-1012-2 Find more CRISPR Resources...

  8. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...the more detailed questions the organizers wanted to touch on in organizer David Riglar’s blog post. Essentially...Symposium On the FOR website, the FOR Boston 2015 organizers indicate that the main question they wanted to...panel was that there are many companies and organizations (including Addgene), that need people who are...

  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...in the comments or shoot us an e-mail at blog@addgene.org and maybe we can write a blog post about it! ...including subcellular visualization of 6 different organelles, a cell cycle indicator for mouse embryonic stem...mitochondrial fusion with a new tri-chromatic assay Georg Ramm’s lab at Monash University has developed a ...viability. In their recent work in Nature Methods, Georgieva et al describe the rational production of a new...have fewer effects when fused to your POI. Georgieva et al developed the inntags via a bioinformatics...information on inntags, read our recent blog post. Georgieva, et al. Nature Methods. 2015 Aug 31. CRISPR-Display...monitoring cargo trafficking, and for discovering organelle contact sites. This suite of deposited fluorescent...

  10. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...has also been implemented in a variety of other organisms, such as sphingomonads and bacillus.  ADH1 The...mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750-6-43Kaczmarczyk A, Vorholt JA...overexpression transgenes. Sci Rep 12, 3835 (2022). https://doi.org/10.1038/s41598-022-07852-7 De Boer, Herman A., ...and PolyA Signals FLEx Vectors Resources at Addgene.org Tetracycline Collection ...

  11. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...Cell Culture. Anal Chem 84:4227–4232 . https://doi.org/10.1021/ac2033112 Ligasová A, Vydržalová M, Buriánová...Fluorescence Microscopy. Cells 8:1510 . https://doi.org/10.3390/cells8121510 Sung H, Kang SH, Bae YJ, Hong...Lines. In Vitro Cell Dev Biol Anim 38:86 . https://doi.org/10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2...for working with viral vectors Resources on Addgene.org Check out Addgene's written and video protocols...

  12. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...Psychological and Personality Science 11:647–657 . https://doi.org/10.1177/1948550619882032 Chrousos GP, Mentis A-...threatens diversity. Science 367:749.2-750 . https://doi.org/10.1126/science.aba8039 Cordova FA (2016) Embrace...Embrace uncertainty. Science 351:994–994 . https://doi.org/10.1126/science.351.6276.994 Moore E (2021) One...I built a network of mentors. Science. https://doi.org/10.1126/science.caredit.abi6939 Additional resources...

  13. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... blood stream to commute to and invade multiple organs, and most importantly escape the immune mechanisms...environments for tumor cells spreading to other organs during metastasis. Evading Recognition Tumor cells...2002): 991-998. PubMed PMID: 12407406. 4. Weiner, George J. "Building better monoclonal antibody-based therapeutics...Ghana Modulate Cancer Signalling Resources on Addgene.org Check out Our Cancer Collection Pages Use the...

  14. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...of Scientific Instruments 43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow Cytometry...Current Protocols in Immunology 120: . https://doi.org/10.1002/cpim.40 Picot J, Guerin CL, Le Van Kim ...instrumentation. Cytotechnology 64:109–130 . https://doi.org/10.1007/s10616-011-9415-0 Additional resources ...proteins Learn more about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody...

  15. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    ...of a lawyer can bring a new perspective to an organization. I'm used to thinking about the downstream results...how to manage them without them paralyzing an organization. I think these are things that I can bring to...that business experience and judgement to the organization and see how I can help. Q: What do you enjoy...

  16. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    ...research with the community, so email us at blog@addgene.org if you are interested. Addgene's Most Popular...Plasmids 101: Origin of Replication By Kendall Morgan Save Time and Money by Making Your Own Competent...101: The Promoter Region – Let's Go! By Kendall Morgan Plasmids 101: Mammalian Vectors By Marcy Patrick...

  18. Let There Be LITE Plasmids

    Type
    Blog Post
    ...tissue. “The brain is such an amazing and dynamic organ,” Konermann explained. “It helps us adapt to influences...use is by no means limited to particular model organisms or cell types. “This tool is really flexible and... Kendall Morgan...

  19. Reflections and Looking Ahead

    Type
    Blog Post
    .... Even more remarkably, 1,160 labs from 522 organizations in 41 countries enriched our repository with...antibody characterization. Nature Protocols. https://doi.org/10.1038/s41596-024-01095-8 Kahn, R. A., Virk, H...biomedical research. eLife, 13, e100211. https://doi.org/10.7554/eLife.100211...

  20. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...growth, survival, and differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs/ Finding...Technologies from 2015 Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled... Kendall Morgan...
Showing: 381 - 400 of 949 results