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We narrowed to 511 results for: plasmid dna

Showing: 381 - 400 of 511 results

  1. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Tet-Off system.  For more information on Addgene Tet plasmids, see our Tetracycline resource page. Cumate Switch...binding to the lexA operator (lexAop). Fusing the LexA DNA-binding domain (DBD) to GAL4 or VP16 activation domain...20697123. PubMed Central PMCID: PMC2942869. Find plasmids from this publication at Addgene. del Valle Rodriguez...20434990. PubMed Central PMCID: PMC2883883. Find plasmids from this publication at Addgene. Wei, Xing, ...22406855. PubMed Central PMCID: PMC3846601. Find plasmids from this publication at Addgene. Riabinina, ...25581800. PubMed Central PMCID: PMC4344399. Find plasmids from this publication at Addgene. Riabinina O...Hill, C. et al. A LexAop > UAS > QUAS trimeric plasmid to generate inducible and interconvertible Drosophila...

  2. TALEN Plasmids and Kits

    Type
    Collection
    ...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...enhancers in mammalian cells. These plasmids allow for cloning DNA fragments encoding TAL effector repeat...TALE Toolbox 49622 - 49647 24 plasmids Thoru Pederson TALEColor plasmids: destination vectors for use ... repetitive DNA sequences in human chromosomes Do you have suggestions for other plasmids that should ...module plasmids and improve the success rate of Golden Gate assembly. Destination vectors, pcDNA-TAL-NC2... TALE plasmids. Genome...

  3. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...In this post, we’ll summarize how IDT (Integrated DNA Technologies) first demonstrated how CRISPR nickases...Check out CRISPR Topic Page Find CRISPR Nickase Plasmids Browse the CRISPR Guide ...insertions (long ssDNA; IDT Megamer® ssDNA Fragments) Test both bottom and top strand ssDNA donors (if possible...longer insertion (mCherry) using IDT Megamer long ssDNA with 100 nt homology arms. Schubert and Yan next...

  4. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...popular split-FP plasmids from our catalog to help you find the right split-FP plasmid for your experiment...below, and click the Article link to find all the plasmids from the associated paper:   Color Base FP...Constant rate of p53 tetramerization in response to DNA damage controls the p53 response. Gaglia G, Lahav...and experimental design. We hope you find these plasmids useful. Good luck, and happy experimenting! References...

  5. CRISPR Plasmids - Base Edit

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    Collection
    ...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break....CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... by the Doudna Lab . Browse, sort, or search the tables below for CRISPR base editing plasmids. Plasmids..., creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature, but have been ...

  6. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ... entity, please see our Plasmid Distribution to Industry page. These plasmids have not been expressed ...Addgene. Plasmids Use the search bar or sort buttons to find SARS-CoV-2 and common coronvirus plasmids based...SARS-CoV-2 Plasmids for Many Expression Systems Collection: COVID-19 and Coronavirus Plasmids & Resources...Browse Ginkgo Bioworks plasmids to express SARS-CoV-2 viral proteins in several expression systems. Depositor...Ginkgo Bioworks COVID-19 Ginkgo Bioworks COVID-19 Plasmid Collection (Link opens in a new window) Researchers...therapeutics, and vaccines against COVID-19 all rely on DNA encoding viral proteins as a first step for many ...Bioworks (Link opens in a new window) used their DNA design and synthesis capabilities, in collaboration...

  7. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... brightness and high degree of overlap. Dyes for DNA/RNA staining should have high affinity and specificity...specifically to the AT-rich regions of double-stranded DNA and exhibits a blue-fluorescent light when excited...cell and protein analysis products. You can find plasmids deposited by Promega here.    References and ...Self-Labeling Protein Tags Fluorescent protein plasmids and resources A practical approach to choosing...

  8. Adenovirus Plasmids

    Type
    Collection
    ... Viral Plasmids Adenovirus Plasmids Adenovirus Plasmids Additional Resources: Adenovirus...yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined...recombined into a DNA molecule that incorporates sequences from both plasmids. This DNA molecule can then...contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral... Venus tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral Kits Plasmids containing modular...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are...Strain for recombination between shuttle plasmid and adenoviral plasmid Vogelstein 16399 AdEasier-1 BJ5183 ...

  9. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...fusion proteins are made by connecting its DNA code to the cDNA encoding for another protein. Effectively...cells, but not other biomolecules of interest e.g. DNA, RNA, and lipids. Still, these molecules can be indirectly...protein fusions Browse the Fluorescent Proteins Plasmid Kits ...

  10. Protocol - How to Design Primers

    Type
    Protocol
    ...fragment of DNA. On the other hand, a long primer is used to amplify a eukaryotic genomic DNA sample. However...You may also like... Polymerase Chain Reaction Plasmid Cloning by PCR Agarose Gel Electrophoresis Primer...that are complementary to the template region of DNA. They are synthesized chemically by joining nucleotides...the primer corresponds completely to the template DNA strand so elongation can proceed. Usually a guanine...short. Short primers produce inaccurate, nonspecific DNA amplification product, and long primers result in...in a slower hybridizing rate. On average, the DNA fragment that needs to be amplified should be within...

  11. p53 Pathway

    Type
    Collection
    ...function in cancer. p53 Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor...mTOR pathway Ras pathway Background p53 Pathway Plasmids p53 Gene List References Background p53 is a transcription...tumor suppressor activated by cellular stresses like DNA damage, oxidative stress, and nutrient deprivation... negative regulators, increase its stability and DNA binding activity, and allow it to bind transcriptional...of these mutations are missense mutations in the DNA-binding domain, often at one of six hotspot residues...

  12. Plan Your Experiment

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    Collection
    ...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...more than just the DNA sequence. If this is the case, you can confirm your edit using DNA-based methods like...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells...mutations or small tag insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins...different activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your gRNA ...efficiency . Remember that HDR also requires donor DNA to serve as template for the repair. Once you settle...

  13. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...Novel techniques for the prevention of mitochondrial DNA disorders.” This document is about the popular technique...Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Check out our genome engineering guide...scientific possibility, safety, and efficacy of the mtDNA transfer options being investigated; A UK regulatory...

  14. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...transducing primary cells. What if, instead of supplying a DNA construct, they could simply induce cells to take...10.1016/j.cell.2015.03.028. PubMed. Find the Plasmids @Addgene: iTOP-tested proteins and reporters...

  15. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...CRISPR plasmids that create double strand breaks in DNA....Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert... elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Xenopus ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...changes, researchers use ssDNA or dsDNA repair templates with 1. homology to the DNA flanking the DSB and ...

  16. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...Virus-associated DNA Each viral vector service request will also include virus-associated DNA, which is a ...a sample of the purified DNA from the original plasmid that was used to make the virus. In order for Addgene...the right to use the original plasmid. Distribution of the original plasmid is important because it is the...Overview of Addgene's viral preparation service for plasmids and pooled libraries. Viral preparation options...produces high-quality viral vector preps from select plasmids in our collection. We know that viral vector production... production is a common step after requesting plasmids from Addgene, and by providing this service, we...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

  17. Viral Production

    Type
    Collection
    ...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral...Transfer Plasmid Addgene uses a rigorous barcode matching system that follows the sample from the DNA tube...cells several days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...of the viral genome and the absence of unexpected DNA contaminants. The data is also used to determine ...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...lentiviral vector, incubated for 72 h, and genomic DNA is extracted. Primers and probes targeting integrated...

  18. Deep Dive: qPCR

    Type
    Blog Post
    ...single-stranded) DNA. As the temperature drops, single intensity should smoothly increase as the DNA amplicon ... are produced by the presence of double-stranded DNA, when temperatures are high, signal intensity should...dyes that are not dependent on amplification of the DNA; they are, however, affected by bubbles in wells,...LightCycler qPCR optimisation for low copy number target DNA. Journal of Immunological Methods 270 (2002) 119 ...is a smooth, single-step transition between ssDNA and dsDNA. This is not true for all amplicons; if your... probes (which you probably know as TaqMan) and dsDNA dyes (commercial name: Sybr). However, there are... Yes Yes Agarose gel or uMelt analysis dsDNA dye (Sybr)  High No No No Melt curve ...

  19. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...... Restriction Digest of Plasmid DNA Purifying DNA from an Agarose Gel DNA Ligation Introduction Gel ...length of a DNA fragment by running it on an agarose gel alongside a DNA ladder (a collection of DNA fragments...negatively charged DNA through an agarose gel matrix toward a positive electrode. Shorter DNA fragments migrate...100 mL gel). EtBr binds to the DNA and allows you to visualize the DNA under ultraviolet (UV) light. Caution...from the DNA. So if you run the gel without EtBr in the buffer you will reach a point where the DNA will ...that has UV light, visualize your DNA fragments. The fragments of DNA are usually referred to as ‘bands...or c) loading less DNA into the well. Another method for visualizing very short DNA fragments is polyacrylamide...

  20. Serotype Testing AAV

    Type
    Collection
    ...virus associated DNA (vdna), which is a sample of the purified DNA from the original plasmid that was used...AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV in various... for direct injection. These AAV are made from plasmid 37825 (deposited by Edward Boyden ) and direct ...AAVrg.T 20 µL $ 150 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use AAV in various... for direct injection. These AAV are made from plasmid 50465 (deposited by Bryan Roth ) and direct EGFP...details, see our FAQ on What is virus associated DNA, and why do I have to order it? Don’t See What You...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....
Showing: 381 - 400 of 511 results