We narrowed to 883 results for: prot

...Technique: Probe Phage Genomes for Host Binding Proteins & Running for Rare Disease, Running for FOP, Running...Technique: Probe Phage Genomes for Host Binding Proteins," was contributed by guest blogger Jessica Sacher...describes a technique you can use to find phage proteins that bind to your bacterium of interest. For those...an ingenious but simple technique to screen for proteins that could be useful for a wide variety of reasons...

...Davidson Fluorescent Protein collection, a ‘One-Stop Shop’ for Fluorescent Proteins, and plasmids from ...for tagged and untagged expression of SARS-CoV-2 proteins in bacteria, yeast, and mammalian cells. We teamed... determine the three dimensional structures of proteins.  The RESOLUTE Consortium collection enables research...

...include enrichment against kinases, cell cycle proteins, and more.  “In the past, genome engineering with...leveraged the CRISPR system to target all 19,150 mouse protein coding regions with 87,897 unique gRNAs. Their ...aid efforts to understand the function of both protein-coding genes as well as non-coding genetic elements...might need larger numbers.” Further details and protocols are available online on Addgene's CRISPR Library...

...an affiliated TniQ protein recruits transposition cofactors. The transposon proteins integrate a payload... combines with a donor template and transposon proteins (Tns) to integrate large sequences into genomic... using CRISPR-associated transposases. Nature Protocols, 19(3), 752–790. https://doi.org/10.1038/s41596...

...advantage of bacterial characteristics such as prototrophy, the ability to synthetize all compounds needed...synthesize a compound needed to grow. Auxotrophy and prototrophy come into play during cloning if you’ve designed...example, Markus Ralser’s lab created a yeast prototrophy kit containing 23 plasmids to complement auxotrophy...molecular biology reference Watch molecular biology protocol videos ...

...Science Resources on Addgene.org Find Protocols and Protocol Videos Check out LeGO plasmids from the... very human side to scientific research. Find Protocols Lab Tips to Keep Your Experiments Running Smoothly...this world where we are often trained to follow protocols, guidelines, and standards. Bricking science reminded...

...guest blogger Meghan Monroy, a graduate student in Protein Science at the University of Connecticut. Molecular...at the University of Connecticut interested in protein science.     References: 1. Celie, Patrick HN, ... Perrakis. "Recombinant cloning strategies for protein expression." Current Opinion in Structural Biology...Empty Vectors for Your Research Find Additional Protocols Browse Our Special Collections for Plasmids from...

...aminoglycoside Binds 30S ribosomal subunit; interrupts protein synthesis Bactericidal 7.5-50 ug/mL Streptomycin...Streptomycin aminoglycoside Inhibits initiation of protein synthesis Bactericidal 25-100 ug/mL Ampicillin...tetracyclin Binds 30S ribosomal subunit; inhibits protein synthesis (elongation step) Bacteriostatic 10...Molecular Biology Reference Pages View our Plasmid Protocols Find Your Perfect Plasmid Backbone ...

...system was enhanced to allow searches for a perfect protein sequence match even when the DNA sequence has changed... inaccurately. The best example is fluorescent proteins, which often come in closely related versions ..., we augment the database with new fluorescent protein variants. The future of DNA feature identification...Collections”, which are shareable databases of DNA and protein sequences, the next step is to enhance Collections...

...sequences is Proteobacteria - the most frequent transfer of genes is from Proteobacteria to mammalian ... they use; even if two species encode the same protein, their gene sequence may have slight or large variations...resembles that of the host organism, it may express protein more efficiently. To classify gene sequences as...

...libraries In surface display, proteins of interest are fused to anchoring proteins to be expressed on the surface...systematically explore variations in the spike protein, teaching us more about viral evolution and antibody... Pooled Libraries CRISPR Library Amplification Protocol ...

...nuclease that is active up to 100°C.  The IgnaviCas9 protein is 1,241 amino acids long, has optimal sgRNA spacer...CRISPR nuclease activity is desired and time or protocol steps can be saved by flexibly deploying IgnaviCas9...without an increase in the number of steps in the protocol. IgnaviCas9’s incredible thermostability makes...resources on the Addgene blog Learn about other Cas proteins Read all of our CRISPR blog posts Read about GeoCas9...

...including a link to a detailed CellTag protocol on protocols.io, code and analysis tutorials on GitHub...Hnf4α, a process that like other reprogramming protocols is inefficient. Cells were sequentially tagged...factors for generating iPSCs Learn how fluorescent proteins help researchers study single cell dynamics in...

...Plasmid Cloning Protocols Catch Up On Your Basic Molecular Biology Techniques Watch Our Protocol Videos ...interest with Taq polymerase using your favorite PCR protocol.  2. Set Up TOPO Cloning Reaction: Mix together...Competent Cells: You can use your standard lab protocol for this; however, you should reduce your incubation...

...colleagues began by isolating a calcium-binding protein, troponin C (TnC), from muscle cells. They identified...calcium binding and developed a version of the protein with as few calcium-binding sites as possible for...transcription factor cAMP-responsive element-binding protein (CREB) in living cells. “Now we are making this...Eindhoven has similar interest in FRET-based sensor proteins, particularly for the intracellular imaging of...

...gramstain.html Smith, A. & Hussey, M. Gram Stain Protocols. American Society for Microbiology (2005). https...5c95a063-326b-4b2f-98ce-001de9a5ece3/gram-stain-protocol-2886.pdf Steward, K. Gram Positive vs Gram Negative...Hans-Christian-Gram.pdf Smith, A. & Hussey, M. Gram Stain Protocols. American Society for Microbiology (2005). https...

...performance of antibodies, because of effects of pH on protein stability (Murphy et al., 2013). However, the pH...Mueller BM, van der Geer P, Manning MC, Fitchmun MI. Protein instability following transport or storage on dry...Byrne BJ, Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors...Addgene.org Check out the Viral Service Find Virus Protocols Browse Our Viral Vectors ...

...standard lentivirus production protocol here and you can find more tips and protocols for working with lentivirus...researcher can complete an entire lentiviral production protocol only to find that the low titer of her prep makes...lentivirus on our blog and protocol pages. We’re excited to provide this new service for researchers wanting...

...organized allowing you to upload anything from protocols to pictures of gels or even sketches to your notebook...but the NEB Tools app comes in handy when I use protocols that require restriction enzymes or PCR. The app...Bioinformatic Analyses Use Apps to Share Your Lab Protocols Check Out How Addgene Develops Lab Management ...Management Software Resources on Addgene.org Find Protoctols for Your Research Catch Up On Your Molecular Biology...

...nucleofection, which introduces functional Cas9 protein and the targeting guide RNA into cells by electroporation...extremely high frequencies of HDR. The detailed protocol for nucleofection as well as the sequences of ...is relatively modest, requiring expressed Cas9 protein, transcribed sgRNA, and optional donor DNA. Chris...human cells via delivery of purified Cas9 ribonucleoproteins." Genome research 24.6 (2014): 1012-1019....