We narrowed to 545 results for: SEC

1. Celebrating 15 Years of Scientific Sharing

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   Blog Post

   ...Addgene is about half of what is standard in most sectors. The Addgenies support a high collaboration, high...

2. Troubleshooting Your Plasmid Cloning Experiment

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   Blog Post

   ...the above is correct, you should probably have a second and more detailed look at your experimental design...

3. Summer SciComm Series: Cognitive Load

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   Blog Post

   ...We are now at the second post in our Summer SciComm series and it’s time for one of my favorite scicomm...

4. What's New in CRISPR - November 2021

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   Blog Post

   ...demonstrate SEND as a modular system for packaging, secretion, and delivery of specific RNAs, such as Cre mRNA...

5. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   Blog Post

   ... used primary antibodies against mCherry, and secondary antibodies coupled to nanogold particles, that...

6. dTAG - You're it!

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   Blog Post

   ...these plasmids are available here. The Methods section of Nabet et al., Nature Chemical Biology additionally...

7. Visualizing Protein Turnover In Situ

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   Blog Post

   ...was directed toward a protein of interest but the second antibody was directed toward AHA. Now the rolling...

8. Human Germline Editing Using CRISPR

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   Blog Post

   ...edited cells. The results were even lower for the second gRNA: 13.1% targeting efficiency with only 13% ...

9. Lighting Up Cell Signaling with Photoswitchable Kinases

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   Blog Post

   ...of 500 nm light (turn on psRaf1) followed by 3 seconds of 400 nm light (turn off psRaf1). After this, ...

10. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    Blog Post

    ...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...

11. CRISPRainbow and Genome Visualization

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    Blog Post

    ...gRNA with two different hairpins will produce a secondary color cyan, yellow, or magenta, depending on the...

12. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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    Blog Post

    ...who had a contract - negotiated by their union - securing higher salaries. This decision will carry over...

13. Addgene's Tips for Plasmid Quality Control

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    Blog Post

    ...your favorite sequencing tricks in the comments section below.  Additional Resources on the Addgene Blog...

14. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    Blog Post

    ...TadA from the complex the researchers developed a second set: The ABE8.x-d version carries a fusion of ...

15. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    Blog Post

    ...promoter to drive expression of Cre-mCherry and a second promoter to drive expression of a Cre-dependent...

16. How to Prepare for an Industry Interview

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    Blog Post

    ...Maybe you ask for questions after completing each section of your talk. Some video conferencing software ...

17. Open Resources and Plasmid Tools For Studying C. elegans

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    Blog Post

    ...PMC5693259.  Wang, Han, et al. "Split cGAL, an intersectional strategy using a split intein for refined spatiotemporal...

18. Mouse Modeling, Part 1: Genetically Engineered Mice

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    Blog Post

    ...engineered mouse seems less overwhelming! In the second blog post of this series, I’ll discuss crossing...

19. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    Blog Post

    ...experiments performed will be useful in perpetuity.  Second, plasmids are easy to store and share, making recombinant...

20. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    Blog Post

    ...our sequencing results in the Depositor Comments section at the bottom of the plasmid page. Viral preps...