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Showing: 601 - 630 of 858 results

  2. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...important plasmid elements – promoters, origins of replication, protein tags, and antibiotic resistance markers...proteins or co-factors, thus allowing for broad applications in a variety of experiments. LoxP (locus of ...

  4. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...important to consider that the same receptor can elicit different responses depending on what cell type...chemogenetics for research and potential clinical applications. Science 6436: eaav5282. https://science.sciencemag.org...

  6. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts...

  7. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...BioRender.com In their recent ACS Synthetic Biology publication, Mary Dunlop’s lab generated a photosensitive...regions with Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes bringing the C-terminal...

  8. Hot Plasmids - October 2020

    Type
    Blog Post
    ...Jerome Goudeau, Cynthia Kenyon, and colleagues at Calico Life Sciences. Like many split FPs, split-wrmScarlet...Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express hybrid ...

  9. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...experiment and they don't do anything. Viruses are complicated and they perform complex biological tasks, both...vector may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try amplifying...

  10. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...rho-dependent and rho-independent. Rho factor is a helicase which assists RNA polymerase in the termination...Central PMCID: PMC104046. Wodrich H, Schambach A, Kräusslich H-G. Multiple copies of the Mason–Pfizer monkey...

  11. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...typically requires transducing with a limiting multiplicity of infection (MOI) to ensure only one copy integrates...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...

  12. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...these people and bios were likely part of the publicity. Good panels do not waste time on long introductions...on 1-2 panelist for each question in a way that elicits those interesting stories and opinions. Don't let...

  13. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...targeting anywhere in an exon. But things get more complicated if you’d like to use homology directed repair...editing rates. This PAM flexibility should expand applications that require very specific targeting, like homology...

  16. Pooled Library Amplifications

    Type
    Blog Post
    ...to amplify the sample for further downstream applications. As individual libraries can vary significantly...containing smaller, recombined backbones that may replicate faster. For this reason, if your library has inserts...

  17. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ... into Addgene’s CRISPR data? Read our recent publication, “The Expanding Dissemination and Distribution...support for, this and previous Addgene CRISPR publications.  References and resources References Pyhtila...

  18. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...moments. The models function as thinking tools – eliciting questions like “what does this model representt...or trying to explain your science to the general public, visit the Center for BioMolecular Modeling web...

  19. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ..., and making sure we always maintain a backup replicates of each clone in case of contamination. We do...population of transfected clones. The technique is applicable to a wide variety of cell lines, from HEK to ...

  20. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ..., blastp, and blastx searches! Now, before you click the BLAST button, consider the Program Selection...may have noticed the “Algorithm parameters” link. Click on this link to view advanced BLAST options and ...

  21. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...-1250015778 7. Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina...

  23. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...are often associated with rote memorization of metabolic pathways (precisely the type of exercise Einstein...mitochondrial MDH (hMDH2) with those from human cytosolic MDH (hMDH1). They then expressed the original ...

  25. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...be part of the answer. Problems with written publications Online tools like ResearchGate, GoogleScholar...Freedom: The Expansion of Data Sharing in Research Publications Resources at Addgene.org Check out our Protocol...

  26. Hot Plasmids - August 2020

    Type
    Blog Post
    ...protein evolution By Joanne Kamens Mateo Sanchez and Alice Ting have used one biotechnology tool to optimize...cultured and FACS sorted. Image from Mateo Sanchez and Alice Ting. Sanchez MI et al., Nat methods. 2019. https...

  28. Bioinformatics at Addgene

    Type
    Blog Post
    ... from depositing laboratories and associated publications, but our quality control (QC) standards have...can be easily shared with others without any complicated setup. We created, tested, and debugged each ...
Showing: 601 - 630 of 858 results