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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 921 results for: Tro;

Showing: 601 - 630 of 921 results

  1. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ... light in the near-infrared portion of the electromagnetic spectrum – the spectral region in which mammalian...Nature Communications last year, “The capability to control spectral properties of PA FPs with light of specific...

  2. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...policy gives us time to complete necessary quality control and legal approvals so the plasmids are ready for...start out with this hold! You will always have control over when your plasmids are distributed. Next month...

  3. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...You’ve designed the perfect experiment – controls, conditions, and everything in between – now all you... Susanna Stroik...

  5. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...a reporter signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent... being especially popular. We’ll give you an introduction to fluorescent biosensors, but keep in mind ...

  6. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...Each plasmid page now has a helpful graphic to introduce you to the plasmid class covered on that page....Using CRISPR/Cas9 and Plasmids 101: NGS Quality Control for Pooled Libraries Day 12: New website resources...

  8. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...cell types. MEFs were reprogrammed into iEPs by retroviral expression of transcription factors Foxa1 and.... Adding Mettl7a1 to the standard Foxa1-Hnf4α retroviral overexpression cocktail yielded a three-fold ...

  9. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ... A fluorescent probe that releases an internal control Another autophagy biosensor is the GFP-LC3-RFP-LC3ΔG...it remains in the cytosol acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio...

  10. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...endogenous IgGs, you should include no primary antibody controls in your experiments. If you do find high levels...resources on the Addgene blog Antibodies 101: Introduction to Immunofluorescence Antibodies 101: Affinity...

  11. What the HEK?

    Type
    Blog Post
    ...Culture Adenoviral Vector Production and Troubleshooting Additional resources on Addgene.org AAV ...  Susanna Stroik...

  15. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...build, sales and marketing gives you a great introduction to the financial world. It teaches you about...you can find some great information on ELISA troubleshooting, ELISA kits and cell death assays. You can ...

  16. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...complex genetic diseases, Alzhiemer’s disease (AD).  Troy Rohn and his lab are researching a way to treat ...proteins aid the limitation of these mutations, to control the CRISPR system activity.  Using an AAV vector...

  17. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...person or virtually) will make the relationship stronger.  If appropriate, invite them to speak for your...chocolate-covered strawberries for helping with an introduction that helped someone get a job interview. Even...

  18. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...Nsp5. Our set also includes a GFP construct as a control. For easier portability, all viral genes are flanked...clone selection. Expression is driven by a fairly strong EF1alpha promoter. We have verified expression ...

  19. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ... one figure. If you aren’t there yet, write introductions to potential publications or prepare a review...isn’t for a while. Getting started early on introductions and literature reviews could make the months...

  22. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...YOUR PLASMID has been developed to teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies...at FRESCI particularly interested in advanced in vitro models technology. Follow him on twitter @FRE_SCI...

  23. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...necessarily have to be. Regions of homology can be introduced to fragments via PCR using primers that contain...patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. Many thanks to ...

  24. Cultivating Community Science at BosLab

    Type
    Blog Post
    ... the lab, so in December 2015 we held our first intro to bacterial transformation course (read our blog...bacterial transformation guru and instructor for our intro to biohacking courses. Our members pursue a diverse...

  25. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...you did. Emphasize the skills. If you studied astronomy or bioinformatics data, then the chance is that...Communications Coordinator for the Department of Physics & Astronomy, University of British Columbia. Originally on...

  27. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...equipment always helps. But how does one build a strong collaborative research team motivated by similar...selectively picking the first couple of hires to build a strong, collaborative team. And take advantage of the ...

  29. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...Please keep in mind that this is just a brief introduction to the wide, wild, and fascinating field of ...Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies Resources on Addgene.org ...

  30. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ..., you’ll want the proper positive and negative controls for your PCR.  Find the details and learn more... this blog post for tips for analyzing and troubleshooting Sanger sequencing results. At Addgene, we now...
Showing: 601 - 630 of 921 results