...little screening for DNA markers like the presence of high risk (HR) Human Papillomavirus (HPV). As Gladys ...individuals who are infected with HR HPV and therefore at high risk for cervical cancer development and also better...disease severity. Individuals who are shown to be at high risk by these screens can then undergo more routine...

...service pAAV_hSyn1_nLightG AAV9 Biosensors Patriarchi New viral service pAAV_hSyn1_nLightR AAV9 Biosensors...of synapses distant to the site of injection with high temporal precision.   Table 1: New ready-to-use...

...targeted destruction.  Antibodies are composed of two light chains and two heavy chains. The ends of the branches...branches contain the antigen binding sites and are highly variable, allowing exquisite specificity. This ...rabbit antibodies that exhibit increased avidity to high profile antigens; and 4) generating mAbs against...

...accelerate research and discovery by improving access to high-quality scientific materials and information. Our... repository of valuable research materials that might otherwise be lost amid lab closures or project terminations...Providing essential services: We continue to offer high-quality DNA sequencing, viral vector preparations...

...only two options, but very little data on which might work in your application. It can be quite difficult...materials and methods section. This section will vary slightly depending on the application — flow cytometry,...Antibodies 101: Validation Antibodies 101: Selecting the Right Antibody...

... a few easy and fast things you can start doing right away to reduce your consumption in the lab: Turn...your oven on all day to save time making dinner at night, so why leave your lab equipment on? This applies...fact, some scientists speculate the -80 °C setting might have been a marketing campaign! MIT Green Labs has...

...look and feel of addgene.org. Some you’ll notice right away, while others may be less obvious — but every...you can use the links under the search box to go straight to your materials of interest!   Figure 1: The...bar New menu Most common activities can be done straight from the new search bar, but the menu just above...

...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....recognizes a simple dinucleotide PAM (N4CC), lending to high target site density (average every 8 bp). Nme2Cas9...

... also selected for other key properties, notably high solubility, limited affinity for other proteins,...interactions, particularly in single-molecule or high-throughput applications. Perhaps IT5 and IT6 will...Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian...

... (top right corner of this post) is an example of a seemingly clean DNA sequence (no Ns in sight). If ...clean reliable DNA sequence Anything less and you might suspect contamination in your sample or consider...

...physicists, chemists, and mathematicians. We hope to highlight every science field as we expand and encourage... This would help you direct your content to the right audience. Avoid using scientific jargons. Someone...

...pleasure to announce that we once again reached new heights on the Addgene blog: we surpassed 60,000 views ...researcher at Sanofi Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia ossificans progressiva...

...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....identified 67 genera of Proteobacteria that this toolkit might be used in. Read the paper in ACS Synthetic Biology...

...science accessible in one way or another to those who might be in a position to build on that work. That’s how...to consider where the open access (OA) movement might be going is to consider some of the places it’s ...providers and participation from many well-respected and high-profile universities. We particularly recommend ...

...functionally validating the thousands of mutations that might play a role in the progression of cancer are often...mask weaker ones. Thus, the need for a faster and higher-throughput in vitro approach. Using data from The..., they generated lentiviral constructs using the High-Throughput Mutagenesis and Molecular Barcoding (...

...directory is crucial because it's hard to find the right phage for the job. Phages are viruses that can infect... have someone that really needs it, but then it might to be too late,” Sacher says. As the labs in the...sample is limited, only the labs that have the highest potential to possess the correct phages will test...

...excitation at 488 nm, photoconverted with 405 nm light, and then captured in the red state after excitation...photoconverted, so this technique may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have...Murphy CS, Hazelwood KL, Davidson MW, Looger LL. A bright and photostable photoconvertible fluorescent protein...

...research and expanded choice when it comes to ordering high-quality materials from the Addgene repository. Read... be aware that this ordering process will look slightly different from our standard process. We’ll walk... and flexibility; while ensuring we can maintain high standards for both our depositors and requestors...

...within the scientific community The team behind Crowdfight COVID-19 strives to match up scientists across...with different technical knowledge that could help fight COVID-19. They’ve also collated a list of useful...  "We realized that there is now a huge pool of highly skilled scientists, willing to volunteer their ...

...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....that controls nuclear localization (LINuS). Blue light renders the NLS accessible and the complex enters...

...on addgene.org. Additionally we also wanted to highlight our blog, daily updates, and offer better exploration...mostly see a friendly smile, but occasionally you might notice Blugene looking sad, especially if an experiment...

...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....and human cells. Using the structure of CasX at eight different states of assembly from cryo-electron ...

...efficient capture. We wanted to be able to build a highly multivalent assembly connected by covalent bonds...St. Andrews discovered the domain. It is small, highly stable and has this amazing chemistry locking the... the variation in cancer, not just the CTCs with high marker expression level. Labs have been trying different...

... has been the go-to place. In 2012, his National High Magnetic Field Lab worked with an impressive 1,350... Proteins 101: GFP Fusion Proteins - Making the Right Connection.  mEmerald plasmids and many more! Baird...know how all the parts go together, we can fix it. Right now, we have no idea." Now, it will be that much...

...Cas9, then with the sgRNAs, and can result in a higher titer due to the smaller size of the plasmids. ...of selection. Shalem and Sanjana say they kept a high representation of library within cells and aimed... construct, but in some biological systems one “might need larger numbers.” Further details and protocols...

...endophyte screening system and plans to employ the light‐inducible bacterial expression system pDawn to clone... India Dr. Mrinalini Nair, Associate Professor (Right), focuses on molecular biotechnology for improving... Rivera Solís, Associate Professor (above, far right), thrives to make affordable biosensors that detect...

... LEGO and science: The potential is endless One night, while opening a LEGO set with my husband, I thought...that resembles a real laboratory. It only took one night to build the first model, but because we are [email protected]. Who knows, your story might just get “legofied.” Many thanks to our guest blogger...

... available, and have deep tissue penetration and high spatial resolution. By piecing together genes from...are sequentially exposed to acoustic pulses with high enough amplitudes to first collapse arg2 and then...development of ARGs and have already developed a high-throughput screening method for serial acoustic ...

... these results, it’s clear that editing is much higher in the PAM-out configuration. In addition, D10A...D10A editing efficiency is consistently higher than that of H840A, especially with smaller nick distances...cuts close to your insertion site, you can’t obtain high HDR efficiency. Nickases create a staggered cut ...

... Hill. Chromatin immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method... purified for subsequent library preparation and high-throughput sequencing. Improvements to the CUT&RUN...challenging to collect large numbers of cells or when a high degree of signal to noise is desired. It also provides...