Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 772 results for: Dos

Showing: 401 - 420 of 772 results

  1. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...different epitopes on the target protein, so they do not compete with each other. This antibody is also...interest that is present. Using a Standard Curve To do that calculation, you’ll need to include a standard...

  2. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ... only problematic word. How many different things do you simply refer to as a “tube”? We took steps towards...lab or collaborators will not have the context you do, which means you’ll need to provide that context ...

  3. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...had an industry interview before. What should you do to get ready for this interview? Maybe the above ...the same boat, but there are a few things you can do to make the experience less stressful. First, you...

  4. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...ability to target almost every genomic locus, and to do so in vivo. Catalytically dead Cas9 (dCas9) cannot...CRISPRainbow requires very short, 11-mer gRNAs, which do not induce genome editing. If used with catalytically...

  5. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ... all have their benefits and drawbacks. Labs that do not routinely produce virus may want to choose more...Additional Resources on Addgene.org Learn How We Do Viral Production at Addgene Read Our Lentiviral Guide...

  6. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...fragments. When designed correctly, the recognition sites do not appear in the final construct, allowing for precise...these sites are eliminated by digestion/ligation and do not appear in the final construct. The destination...

  8. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...based gene editing. But, how do you decide which tools to use, and what do you need to know about them ...similar to what the endogenous binding partner would do. This allows precise control over when and where ...

  9. Important Considerations When Using AAVs

    Type
    Blog Post
    ...from Addgene 1. Location, location, location! What do buying real estate and using AAVs have in common?...each serotype can transduce multiple tissues. So how do you choose the right serotype? Keep asking questions...injection. Examining serotypes 1, 8 and 9, not only do they undergo retrograde and anterograde transport... transport can be reduced by injecting a smaller dose of AAV and using a serotype with weak CNS penetrance...

  10. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ... human genome (Munoz-Lopez et al., 2010).  So why do transposons matter? The outcome really depends on...how retroviruses, like HIV, replicate. Class I TEs do not encode a transposase enzyme.  Class I TEs are...TEs are also known as DNA transposons, since they do not use an RNA intermediary when they move. Most ...

  11. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...non-coding RNA, cutting most anywhere within the RNA will do. Similarly, if you want to knock out a gene, a frameshift...introduced anywhere within the early coding region will do the job. What matters for these experiments is cutting...

  16. Technical Design of a Western Blot

    Type
    Blog Post
    ...detection range of your protein. The most common way to do this is via a Bradford assay or BCA assay. Be sure...preserve specific protein modifications, we recommend doing a little more research into gel chemistry and/or...wide dynamic range for fluorescent proteins. They do tend to have increased background with higher exposure...your blot to calculate the amount in your sample. (Do ensure your blot is optimized so your standard curve...

  18. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...fluorescence when excited with ultraviolet (UV) light, they do so via aromatic amino acids found in all proteins...photons excite electrons of a fluorophore, they can do so at a range of wavelengths, rather than at just...1, emission spectra of neighboring colors can and do overlap. For example, APC overlaps noticeably with...

  20. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...biochemistry researcher at New England Biolabs. When do you need a high fidelity ligase—and when is an alternative...base pairing through minor grove contacts, and thus do not read specific base sequences, but are sensitive...differential is not known for certain, but may have to do with the slight melting of the 5 ́-terminus during...
Showing: 401 - 420 of 772 results