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An introduction to transposons including Sleeping Beauty, piggyBac, and Tol2 and how they can be used in transposon mutagenesis screens, gene transfer, and creating transgenic animals.

Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.

Learn about some of the factors you should take into consideration when choosing the appropriate AAV for your next experiment.

Learn how the Journal of Emerging Investigators enables high school students to learn more about the scientific process and scientific publishing.

There are a few different types of Fab fragments to consider when you need a smaller affinity reagent than antibodies. Learn about them here!

This post explains how to strip and reprobe a western blot, which is useful for detecting multiple targets or for correcting errors.

A review of alternative PAM and PAM-flexible Cas enzymes.

Learn about a variety of AAV encoded calcium biosensors available from Addgene.

Overview of virus-specific safety controls as well as general safety procedures for viral handling.

ELISA are used to quantitatively detect protein concentrations in experimental samples. Learn about different types of ELISA and when to choose each method.

Find seven tips for scientists when preparing for an industry interview, including advice for remote interviewing.

A toolkit of synthetic promoters for transgene expression in AAV can be used for targeted gene expression in retinal cell types in mice, primate, and human retinas.

This blog posts discusses Cas9 gene drives and the appropriate safeguard and confinement strategies scientists should use for these gene drives.

Western blots have many technical elements. We cover each element, and the different options available to choose from, in this post.

Struggling to get started with designing your first flow panel? This blog walks you through what you need to know and an example panel design to help you get started!

Learn what a high fidelity DNA ligase is and when you might need one.

Get perspectives and possibilities for future action from scientists and other citizens who participated in the March for Science.

Once you create a pooled library, you'll need to ensure it has an amplification protocol. Read our blog on how to develop a protocol for your library!

CRISPR is a story of revolutionary science - but it's also a story of community and sharing. Addgene shares our part in this story.

Our new Instagram series, Meet an Addgenie, features stories form Addgenies about their career decisions, paths, and more.