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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 771 results for: Dos

Showing: 401 - 420 of 771 results

  1. Ras Pathway

    Type
    Collection
    ...Cancer. 2003 Jan;3(1):11-22. PubMed PMID: 12509763 . Do you have suggestions for other plasmids that should...

  2. TALEN Plasmids and Kits

    Type
    Collection
    ...specific repetitive DNA sequences in human chromosomes Do you have suggestions for other plasmids that should...

  3. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...based gene editing. But, how do you decide which tools to use, and what do you need to know about them ...similar to what the endogenous binding partner would do. This allows precise control over when and where ...

  4. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ... human genome (Munoz-Lopez et al., 2010).  So why do transposons matter? The outcome really depends on...how retroviruses, like HIV, replicate. Class I TEs do not encode a transposase enzyme.  Class I TEs are...TEs are also known as DNA transposons, since they do not use an RNA intermediary when they move. Most ...

  5. Important Considerations When Using AAVs

    Type
    Blog Post
    ...from Addgene 1. Location, location, location! What do buying real estate and using AAVs have in common?...each serotype can transduce multiple tissues. So how do you choose the right serotype? Keep asking questions...injection. Examining serotypes 1, 8 and 9, not only do they undergo retrograde and anterograde transport... transport can be reduced by injecting a smaller dose of AAV and using a serotype with weak CNS penetrance...

  6. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...non-coding RNA, cutting most anywhere within the RNA will do. Similarly, if you want to knock out a gene, a frameshift...introduced anywhere within the early coding region will do the job. What matters for these experiments is cutting...

  11. Technical Design of a Western Blot

    Type
    Blog Post
    ...detection range of your protein. The most common way to do this is via a Bradford assay or BCA assay. Be sure...preserve specific protein modifications, we recommend doing a little more research into gel chemistry and/or...wide dynamic range for fluorescent proteins. They do tend to have increased background with higher exposure...your blot to calculate the amount in your sample. (Do ensure your blot is optimized so your standard curve...

  13. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...fluorescence when excited with ultraviolet (UV) light, they do so via aromatic amino acids found in all proteins...photons excite electrons of a fluorophore, they can do so at a range of wavelengths, rather than at just...1, emission spectra of neighboring colors can and do overlap. For example, APC overlaps noticeably with...

  14. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...biochemistry researcher at New England Biolabs. When do you need a high fidelity ligase—and when is an alternative...base pairing through minor grove contacts, and thus do not read specific base sequences, but are sensitive...differential is not known for certain, but may have to do with the slight melting of the 5 ́-terminus during...

  16. Pooled Library Amplifications

    Type
    Blog Post
    ... = 500,000 colonies.  Pro tip! Since you probably do not want to individually count 500,000 colonies, ...did not result in significant recombination. If you do see recombination, we recommend ensuring the cells...

  17. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...use But exactly what kind of editing are people doing with all these CRISPR plasmids (and libraries)? ...scientists use CRISPR technology? We think so — and so do our requestors! CRISPR plasmids in the Addgene repository...

  19. Of Myc and Men

    Type
    Blog Post
    ...Do you ever wonder about the origins of some of the common techniques or tools you use in the lab? Take... to be less sensitive to sequence context.    So, do Schüchner, et al.’s results mean the end of 9E10’...
Showing: 401 - 420 of 771 results