We narrowed to 437 results for: ERG
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TypeBlog Post...site, digestion with the appropriate enzyme will merge two smaller bands visible in a digest of the unmodified...
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Evolution of Lab Techniques
TypeBlog Post...at Harvard University currently studying the serotonergic system and behavior. References 1. Southern... -
Hot Plasmids: Summer 2024
TypeBlog Post...than using more complex protective methods or detergents like CHAPSO, while requiring a lower concentration... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...21561674. PubMed Central PMCID: PMC3159719. 6. Firnberg, Elad, and Marc Ostermeier. “PFunkel: Efficient... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post...before hand, you’re saving the institute time and energy that would typically be wasted going through some... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...-1.0 expresses RFP when the construct has not undergone recombination. This default state was disproportionately... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...publication at Addgene. McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, Kramer A, Martens A, Edwards... -
A Control for All Seasons
TypeBlog Post...identical protocols that the experimental samples undergo. If the antibody is specific, then the target signal... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...you can use Color>Split Channels and then Color>Merge Channels into whatever combination of colors you... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...from AAV2 and engineered to infect neurons and undergo retrograde transport, traveling up the axon to ... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...Goodwin LO, Morgan JL, Juan WC, Roca X, Ong ST, Bergstrom DE (2018) Viable Mice with Extensive Gene Humanization... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post...exonic regions. If the cell’s chromatin structure undergoes significant rearrangement (such as when a stem... -
Plasmids 101: Repressible Promoters
TypeBlog Post....89.12.5547. PMID: 1319065; PMCID: PMC49329. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection... -
Antibodies 101: The Basics of Western Blotting
TypeBlog Post...wet transfer system the sandwich is completely submerged in a tank filled with a transfer buffer formulated... -
CRISPR Guide
TypeGuide...cleavage. Generating a Knockout Using CRISPR Cas9 undergoes a second conformational change upon target binding...stability PEmax — optimized PE architecture; increased synergy between PE enzymes and epegRNAs for enhanced editing... ssisted T argeting), developed by Samuel H. Sternberg’s lab . Many of these systems are based on naturally...argeting E lements), developed by the Abudayyeh - Gootenberg lab , also builds off prime editing. PASTE uses...Welch, M. M., Sousa, A. A., Harrington, L. B., Sternberg, S. H., Joung, J. K., Yildiz, A., & Doudna, J.... S., Okazaki, S., Noda, T., Abudayyeh, O. O., Gootenberg, J. S., Mori, H., Oura, S., Holmes, B., Tanaka...Ran, F. A., Cong, L., Yan, W. X., Scott, D. A., Gootenberg, J. S., Kriz, A. J., Zetsche, B., Shalem, O.,... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...professor at the University of California, Santa Cruz. Emerging CRISPR/Cas9 editing technologies have transformed... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post...Biotechnology. References Degeling MH, Maguire CA, Bovenberg MSS, Tannous BA (2012) Sensitive Assay for Mycoplasma... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH (2018) An Alternate Route for Adeno-associated... -
Transferable Skills Guide: Teamwork
TypeBlog Post...experience on a successful scientific team came as an undergrad at Worcester Polytechnic Institute. Though some... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog Post...CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard...