We narrowed to 430 results for: ERG

1. Antibodies 101: Multiplex Immunofluorescence

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   ...you can use Color>Split Channels and then Color>Merge Channels into whatever combination of colors you...

2. Plasmids 101: Repressible Promoters

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   ....89.12.5547. PMID: 1319065; PMCID: PMC49329. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection...

3. CRISPR 101: Epigenetics and Editing the Epigenome

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   ...publication at Addgene. McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, Kramer A, Martens A, Edwards...

4. Antibodies 101: The Basics of Western Blotting

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   ...wet transfer system the sandwich is completely submerged in a tank filled with a transfer buffer formulated...

5. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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   ...from AAV2 and engineered to infect neurons and undergo retrograde transport, traveling up the axon to ...

6. A Control for All Seasons

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   ...identical protocols that the experimental samples undergo. If the antibody is specific, then the target signal...

7. Generating Mouse Models Using CRISPR/Cas9

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   ...Goodwin LO, Morgan JL, Juan WC, Roca X, Ong ST, Bergstrom DE (2018) Viable Mice with Extensive Gene Humanization...

8. PiggyBac-ing Through the Genome Editing Field

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   ...exonic regions. If the cell’s chromatin structure undergoes significant rearrangement (such as when a stem...

9. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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   ...Biotechnology.       References Degeling MH, Maguire CA, Bovenberg MSS, Tannous BA (2012) Sensitive Assay for Mycoplasma...

10. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    ...AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH (2018) An Alternate Route for Adeno-associated...

11. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    ...professor at the University of California, Santa Cruz. Emerging CRISPR/Cas9 editing technologies have transformed...

12. Live and Let Dye: Self-Labeling Protein Tags

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    ... your own ligands brings up bad memories of undergraduate organic chemistry lab, don’t worry – many SNAP-tag...

13. Transferable Skills Guide: Teamwork

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    ...experience on a successful scientific team came as an undergrad at Worcester Polytechnic Institute. Though some...

14. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    ...CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard...

15. Plasmids 101: Modular Cloning Applications and Kits

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    ...Modular Vector Assembly Enables Rapid Assessment of Emerging CRISPR Technologies. Cold Spring Harbor Laboratory...

16. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    ... FP-protein fusions (FP-chimeras) is that they undergo normal protein turnover. FP-chimeras are continuously...

17. Cancer and the Immune System: Deciphering the Relationship

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    ...elimination by T cells. Immunotherapy strategies that synergize with these positive impacts on the immune system...

18. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    ...liver and ameliorates streptozotocin-induced hyperglycemia. Nat Med, 2000. 6(5): p. 568-72. PubMed PMID...

19. How to Deposit Your Plasmids with Addgene

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    ...notify you that we have received them and they will undergo our quality control process to verify your plasmids...

20. Deep Dive: qPCR

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    ...and experimental parameters (neighbor stacking energies, loop entropy effects, cation concentrations and...