We narrowed to 436 results for: ERG

1. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

   Type

   Blog Post

   ... A. Khavari. "Research Techniques Made Simple: Emerging Methods to Elucidate Protein Interactions through...

2. Five Popular Model Organisms, Part 2

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   Blog Post

   ...blog post to see even more genetic systems for emerging model organisms!) Escherichia coli One of the ...

3. How to Negotiate a Successful Lab Start Up Budget

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   Blog Post

   ...before hand, you’re saving the institute time and energy that would typically be wasted going through some...

4. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

   Type

   Blog Post

   ...-1.0 expresses RFP when the construct has not undergone recombination. This default state was disproportionately...

5. A Control for All Seasons

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   Blog Post

   ...identical protocols that the experimental samples undergo. If the antibody is specific, then the target signal...

6. Antibodies 101: Multiplex Immunofluorescence

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   Blog Post

   ...you can use Color>Split Channels and then Color>Merge Channels into whatever combination of colors you...

7. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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   Blog Post

   ...from AAV2 and engineered to infect neurons and undergo retrograde transport, traveling up the axon to ...

8. Generating Mouse Models Using CRISPR/Cas9

   Type

   Blog Post

   ...Goodwin LO, Morgan JL, Juan WC, Roca X, Ong ST, Bergstrom DE (2018) Viable Mice with Extensive Gene Humanization...

9. Plasmids 101: Repressible Promoters

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   Blog Post

   ....89.12.5547. PMID: 1319065; PMCID: PMC49329. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection...

10. Antibodies 101: The Basics of Western Blotting

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    Blog Post

    ...wet transfer system the sandwich is completely submerged in a tank filled with a transfer buffer formulated...

11. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post

    ...publication at Addgene. McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, Kramer A, Martens A, Edwards...

12. PiggyBac-ing Through the Genome Editing Field

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    Blog Post

    ...exonic regions. If the cell’s chromatin structure undergoes significant rearrangement (such as when a stem...

13. Transferable Skills Guide: Teamwork

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    Blog Post

    ...experience on a successful scientific team came as an undergrad at Worcester Polytechnic Institute. Though some...

14. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    Blog Post

    ...CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard...

15. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post

    ... your own ligands brings up bad memories of undergraduate organic chemistry lab, don’t worry – many SNAP-tag...

16. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    ...AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH (2018) An Alternate Route for Adeno-associated...

17. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    Blog Post

    ...professor at the University of California, Santa Cruz. Emerging CRISPR/Cas9 editing technologies have transformed...

18. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    Blog Post

    ...Biotechnology.       References Degeling MH, Maguire CA, Bovenberg MSS, Tannous BA (2012) Sensitive Assay for Mycoplasma...

19. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    Blog Post

    ... FP-protein fusions (FP-chimeras) is that they undergo normal protein turnover. FP-chimeras are continuously...

20. Cancer and the Immune System: Deciphering the Relationship

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    Blog Post

    ...elimination by T cells. Immunotherapy strategies that synergize with these positive impacts on the immune system...