Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 672 results for: FIE

Showing: 401 - 420 of 672 results

  2. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...sets. Significance is a numerical value which quantifies the likelihood that a result is due to chance...Benjamini uses a complementary approach which identifies a p value cut off. This method entails arranging...

  3. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...In contrast, in the Tet-on system, tTA has been modified (and renamed rtTA) to bind TRE only in presence...Lentivirus Production and Transduction Protocols   Once purified, you must titrate your viral particles to determine...

  4. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...multi-color imaging (Figure 2). If you’re working with purified proteins in vitro, you can even use the same tag...649-mDia1 and 549-CP are both SNAP-tag fusions, purified and labeled separately with different dyes. b)...

  5. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... grad school, you searched for and eventually identified a postdoctoral mentor. A good mentor typically...yourself to and interact with the members of your field, and present your research. Face to face meetings...

  6. Antibodies 101: Validation

    Type
    Blog Post
    ...then you expect that the bulk of the peptides identified to be from the target protein. This approach ...the majority of the protein in the sample was identified as your target protein, allowing you to proceed...

  7. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...the key components of transposons, how they are classified, and how they are used in the lab. What is a ...Figure 2: Overview of DNA transposon transposition. Modified from Sandoval-Villegas et al., 2021. To mobilize...

  8. Typing CRISPR Systems

    Type
    Blog Post
    ... abundant of the two, comprising about 90% of identified CRISPR-Cas in bacteria and nearly 100% in archaea... Witte, I. P., Cofsky, J. C., Kyrpides, N. C., Banfield, J. F., & Doudna, J. A. (2018). Programmed DNA...

  10. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... use antibodies for experiments in a variety of fields including neuroscience and immunology. They can... the antibody using a low-pH buffer. Then, the purified protein can be used in experiments to compare ...

  11. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...introduce. We use Ultramer oligo from IDT (non-PAGE purified oligo is fine based on new data). For general ...homology arms. The Zhang lab typically use PAGE purified long oligos. For large changes (>100 bp insertions...

  12. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...restriction enzyme digestion. However, once you have identified the positive clones, it is important to verify...serum-free media Invitrogen: #31985 Dulbecco’s Modified Eagle Medium (DMEM) Invitrogen: #11995 Fetal Bovine...

  14. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...have some exciting news for you too! The CRISPR field is always changing so we’ve been busy updating our...

  15. The Twelve Days of CRISPR

    Type
    Blog Post
    ... learn about new CRISPR tools. Since the CRISPR field keeps changing, we’ve been updating many of these...

  18. Protocol - How to Streak a Plate

    Type
    Protocol
    ...chance of having a mixture of plasmids in your purified DNA. This protocol explains how to isolate a single...

  20. Call for Guest Bloggers

    Type
    Blog Post
    ...biological scientists from a variety of different fields. Exposure - With over 100,000 views each month,...
Showing: 401 - 420 of 672 results