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  1. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...sets. Significance is a numerical value which quantifies the likelihood that a result is due to chance...Benjamini uses a complementary approach which identifies a p value cut off. This method entails arranging...

  2. Antibodies 101: Validation

    Type
    Blog Post
    ...then you expect that the bulk of the peptides identified to be from the target protein. This approach ...the majority of the protein in the sample was identified as your target protein, allowing you to proceed...

  3. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...the key components of transposons, how they are classified, and how they are used in the lab. What is a ...Figure 2: Overview of DNA transposon transposition. Modified from Sandoval-Villegas et al., 2021. To mobilize...

  4. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...has been optimized for adherent cells but can be modified for suspension cells. The number of monoclonal...the cells are overly confluent or the medium is acidified (i.e., the medium appears orange/yellow if using...

  5. Kit Free RNA Extraction

    Type
    Protocol
    ...protocol for DNA quantification , which can be modified for RNA. Store your RNA sample(s) at -80 °C to...protocol for DNA quantification , which can be modified for RNA.Store your RNA sample(s) at -80 °C to ...

  6. Typing CRISPR Systems

    Type
    Blog Post
    ... abundant of the two, comprising about 90% of identified CRISPR-Cas in bacteria and nearly 100% in archaea... Witte, I. P., Cofsky, J. C., Kyrpides, N. C., Banfield, J. F., & Doudna, J. A. (2018). Programmed DNA...

  8. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... use antibodies for experiments in a variety of fields including neuroscience and immunology. They can... the antibody using a low-pH buffer. Then, the purified protein can be used in experiments to compare ...

  9. Lentivirus ddPCR Titration

    Type
    Protocol
    ...response element (RRE) in the target cells but can be modified for other targets. Primers and a probe against... on their particular sample. This protocol was modified from the publication Wang et al. (2018) . Before...

  10. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...restriction enzyme digestion. However, once you have identified the positive clones, it is important to verify...serum-free media Invitrogen: #31985 Dulbecco’s Modified Eagle Medium (DMEM) Invitrogen: #11995 Fetal Bovine...

  11. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...have some exciting news for you too! The CRISPR field is always changing so we’ve been busy updating our...

  12. The Twelve Days of CRISPR

    Type
    Blog Post
    ... learn about new CRISPR tools. Since the CRISPR field keeps changing, we’ve been updating many of these...

  16. Call for Guest Bloggers

    Type
    Blog Post
    ...biological scientists from a variety of different fields. Exposure - With over 100,000 views each month,...

  18. Summer Fun at Addgene!

    Type
    Blog Post
    ...introducing science communication as a research field. And there will even be a few tips for scientific...

  19. Our New Antibody Mascot is....

    Type
    Blog Post
    ...and received a plethora of options, ranging from Affie to Yncredibody. After an agonizing week spent in...
Showing: 401 - 420 of 665 results