We narrowed to 472 results for: FLU;
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TypeBlog Post... the central nervous system via cerebral spinal fluid. The injection site is the space in and around the...
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PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post...ePRIDICT can help you understand how chromatin might influence editing results at your target. Figure... -
Of Myc and Men
TypeBlog Post...that the amino acids neighboring the Myc epitope influence 9E10’s affinity for the epitope, a phenomenon ... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog Post...through several different applications such as fluorescent protein fusion studies and the generation of ... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post... transgenes. It’s important to note that the fluorescence level observed will be dependent on both the... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...associated, such as when a plasmid contains certain fluorescent proteins. Finally, for many plasmids you can ... -
Genome-wide Screening Using CRISPR
TypeBlog Post... are enriched using either drug selection or fluorescence-based cell sorting and screened for a particular... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...regulation of gene expression including growth or fluorescence. There are a few examples of engineered RNA ... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post... signal amplification in gene expression and fluorescence imaging." Cell 159.3 (2014): 635-646. PubMed... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post...need to use a complementary assay, such as a fluorescence based assay or running an agarose gel. In the... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...Histidine tag, malate dehydrogenase, and green fluorescent protein. Biochemistry and Molecular Biology Education... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...through the cell cycle stages. If cells are overly confluent or don’t have sufficient nutrients in their media... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog Post...history of racial bias, which still impacts and influences current research. Understanding the depth and... -
Tips for arabidopsis transformation
TypeBlog Post...and C terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...critical to stem cell survival. Knowing which genes influence the survival of our cell culture is the foundation... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...protein-DNA and protein-RNA interactions. The 1980s saw a flurry of discovery in the field of eukaryotic transcriptional... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post... is received in the lab, although this number fluctuates throughout the year (see graph below). Addgene... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...mouse adenovirus in mouse; HIV1, West Nile, and Influenza in humans), LY6A lacks a defined role in viral... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...The pooled nature of this experiment requires fluorescence activated cell sorting (FACS) to isolate differentiated... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post...in the Endoplasmic Reticulum, optimization of fluorescent proteins, and live cell imaging approaches. ...