We narrowed to 418 results for: Numb

... {N*F*D\over V_T}$$ Where: T = Titer, TU/mL N = Number of cells transduced F = Fraction of cells with ... = {N*F\over V_V}$$ Where: T = Titer, TU/mL N = Number of cells transduced F = Fraction of cells with ...

... with the appropriate tips, which are made in a number of sizes and colors depending on the pipette they...Although each pipette performs the same function, the numbers are read differently on each type of pipette. This...

...heterogenous polyclonal population that varies in the number of integration events and the site(s) of proviral...cells but can be modified for suspension cells. The number of monoclonal lines generated using this protocol...

...means to scale-up without having to handle a large number of T-175 flasks. Workflow Timeline Day 0: Seed ...would like 2 mg of DNA or 2000 μg. Using the total number of base pairs from all three plasmids, we can determine...

...NGS) for plasmid verification, Addgene has used a number of primers for Sanger sequence verification over...

...individual Level 0 plasmids, or choose from a growing number of libraries containing pre-constructed standardized...

...in trans ). This method, therefore, reduces the number of plasmids that are required in the transfection...

...: MOI (multiplicity of infection) refers to the number of infecting viral particles per cell. Addgene ...following recommendations are guidelines for the number of days you should wait until harvesting your cells...

... temperature. Growth conditions, including copy number and growth temperature, can be found on your plasmid's...

... is important for your experiments. There are a number of factors to consider when selecting sequencing...

...above has a capacity of 200 g, as indicated by the number above the "Zero" button. Before weighing out your...

...baths with instructions on the bottle, for example, number of drops per liter. Place a thermometer in the ...

...dangerous for the user. If you do not have a balanced number of samples, you may need to add a blank. Blanks...

...with normal PCR primers (Gibson et al. , 2010). Number of fragments assembled simultaneously Multiple ...

...about star activity . If you are digesting a large number of plasmids with the same enzyme(s) (for instance...

...transduction units per mL (TU/mL) as follows: TU/mL = number of colonies/total volume in the well (mL) x dilution...

...fractions followed by silver stain. Note the increased number of contaminants in each fraction. * AAV capsid ...

...genome copies and generally represents the physical number of viral particles. Users may need to run lower...