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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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Showing: 401 - 416 of 416 results

  1. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...heterogenous polyclonal population that varies in the number of integration events and the site(s) of proviral...cells but can be modified for suspension cells. The number of monoclonal lines generated using this protocol...

  2. AAV Production in HEK293 Cells

    Type
    Protocol
    ...means to scale-up without having to handle a large number of T-175 flasks. Workflow Timeline Day 0: Seed ...would like 2 mg of DNA or 2000 μg. Using the total number of base pairs from all three plasmids, we can determine...

  3. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...: MOI (multiplicity of infection) refers to the number of infecting viral particles per cell. Addgene ...following recommendations are guidelines for the number of days you should wait until harvesting your cells...

  5. Plan Your Experiment

    Type
    Guide
    ... target DNA may result in reduced cleavage. The number of alleles for each gene may vary depending on ...

  6. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...in trans ). This method, therefore, reduces the number of plasmids that are required in the transfection...

  9. Weighing Reagents Protocol

    Type
    Protocol
    ...above has a capacity of 200 g, as indicated by the number above the "Zero" button. Before weighing out your...

  10. Water Bath Protocol

    Type
    Protocol
    ...baths with instructions on the bottle, for example, number of drops per liter. Place a thermometer in the ...

  11. Centrifugation

    Type
    Protocol
    ...dangerous for the user. If you do not have a balanced number of samples, you may need to add a blank. Blanks...

  13. Gibson Assembly Protocol

    Type
    Protocol
    ...with normal PCR primers (Gibson et al. , 2010). Number of fragments assembled simultaneously Multiple ...

  14. Colony Formation Titering Assay

    Type
    Protocol
    ...transduction units per mL (TU/mL) as follows: TU/mL = number of colonies/total volume in the well (mL) x dilution...

  16. AAV ddPCR Titration

    Type
    Protocol
    ...genome copies and generally represents the physical number of viral particles. Users may need to run lower...
Showing: 401 - 416 of 416 results