We narrowed to 965 results for: REP

...gradients? Excellent! Now, let’s head west, intrepid reader! Protein Prep The first step in any western blot is...minutes) at 37 °C, if you suspect aggregation in your prep. If your proteins are the wrong size on your gel...will simply load an extra ladder or technical replicate (ie, leftover sample) to fill the relevant wells...antibody’s dynamic range. A) The protein dilutions, representing expected concentrations, are all within the ...antibody’s dynamic range. B) The protein dilutions, representing expected concentrations, are outside of the ...blot, you may want to consider using a different reporter or, if you don’t need to compare the protein amounts... amounts directly, stripping and reprobing. Just make sure to go from least to most abundant protein if...

...talk to the occasion Don’t just plan to reuse a prepared talk unless it perfectly fits the occasion.  I...figure out how to make it suspenseful. 4. Start preparing your talk early - like today Don’t wait until ...for poor quality slides with sufficient time to prepare (and it does take time). For important talks, ask...loves a great animation. 8. Predict questions, prepare answers Speaking pros always have a few extra slides...come up with clear and concise answers. This preparation will help you avoid giving only yes or no answers...their phones. Speaking longer just makes you seem unprepared or arrogant. A bit short is always better than...when I then ask “and who has deposited to the repository?” When most or all of the hands go down it is...

...fluorophore-conjugated streptavidin protein, which binds the primary antibody through the biotin-streptavidin interaction...detection. The advantage of this system is that streptavidin conjugates are commercially available for a ...fluorophore selection for panel design. Find streptavidin plasmids and biotin plasmids at Addgene!   ... two-protein labeling by utilizing(a) biotin-streptavidin binding and (b) antibody host species interactions...include transcription factors, histones, and DNA repair enzymes. Note that when using the nuclear fix/perm...or protein-protein interactions, like biotin-streptavidin binding and antibody host species reactivity...

... are overrepresented on Twitter Mathematical, life, and physical scientists are underrepresented. It’s...interested in getting started? Reply in the comments below or tweet us your reply & #scientistswhotweet. And...users. Biologists rank #8. Figure 1: Highly Represented Scientific Disciplines on Twitter. Twitter users...Follower Network of Scientists on Twitter. Each dot represents a scientist. Only scientists who follow each ...

...serotypes at Addgene. Serotype In-stock viral prep Packaged on Request AAV1  ✓ ✓ AAV2 ✓ ✓ AAV2... targeting astroglia. It has additionally been reported to have tropism for liver, vascular endothelial... muscle inflammation and degeneration has been reported (Muraine et al., 2020). AAV8 AAV8 has high transduction... the CNS (Han et al., 2023), and has also been reported to have tropism for liver and kidney cells (Issa...Use Addgene’s AAV Data Hub to find experimental reports on commonly used vector cargo designs and administration...cells utilizing various AAV serotypes. Scientific Reports, 15(1), 20927. https://doi.org/10.1038/s41598-025...AAV Packaged On Request Browse all in-stock AAV preps Browse all AAV plasmids ...

...engineered not to cleave DNA and instead activates/represses gene expression by various mechanisms. The RNA...cleaving of DNA occurs, not for deadCas activator/repressor experiments where only localization of the Cas...will generate a ssDNA break which can easily be repaired by cells with a near zero likelihood of mutation...insertion of DNA by the NHEJ pathway during DNA repair of the break to allow for site-specific detection... these approaches is that NHEJ must perform the repair to allow for detection, not Homologous Recombination...non-cutters If your Cas experiment involves activation/repression or any other effect which does not depend on ... be an appropriate method for an activator or repressor Cas. Similarly, Cas ChIP-Seq would also identify...

...the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. Think...pitch a Hot Plasmids section (antibodies and viral preps welcome!) here. Here's what you'll find in this...Liu Z, et al. Cell. 2022.  Find JEDI-2p viral preps here (and here!)Find JEDI-2p plasmids here!  Liu...adapted from Govorunova et al. 2022.     HcKCRs represent a long-sought tool for researchers to control ...s41593-022-01094-6. (This work was also shared as a preprint: https://doi.org/10.1101/2021.09.17.460684.)   ...

...data repository for validation data on Addgene/CLOVER viral vectors.  Caspedia: A data repository for ... Calculator app for iPhone Reagent and Data Repositories These links can help you find experimental and...for Cas enzymes.  Open repository of CRISPR screens Cellosaurus: A knowledge resource for cell lines IBEX...IBEX Knowledge Base: A data repository for validation data on IBEX reagents.  mAb3D atlas validated antibodies...human, and marmoset brain IHC.  FPbase: A data repository for fluorescent proteins and their properties...

... ReFigure. Reading and exploration including replications and experiments resulting in “negative data”...performance as in this antibody ReFigure Analyzing replications as in this ReFigure about whether Natronobacterium...reagent performs well, or you might publish a replication experiment that you conducted as part of setting...traditional publications. By submitting your data to a repository (Figshare is currently supported by ReFigure ... specialized knowledge of which findings are reproducible. For an example, see our ReFigures on the therapeutic... interim research products (pre-registration, preprints, data, software, micropublications) in grant proposals...

...in the specific repositories (Addgene, WormBase, Arabidopsis.info). These repositories will ensure that...published work meets the accepted standards of reporting in the discipline and reduces the dissemination...information that would prevent the study to be replicated or omission from the literature. I think that...data on ... The language is ... The general representation of data is... The figures are ... In general...the data can be deposited in the public on-line repository (like GitHub, Zenodo, Figshare, Triad) while ...

...efficiency. If this finding is replicated in larger studies, it will represent a key advantage over Cas9, ... RETRACTED AND FOLLOW UP STUDIES HAVE FAILED TO REPEAT THE RESULTS DISCUSSED BELOW Biologists are going...the question on everyone’s minds is: could NgAgo replace CRISPR? Such a drastic shift won’t happen overnight...NgAgo is also able to mediate homology-directed repair when a template is supplied. Find Plasmids from..., this property could be an advantage. However, repair after base excision would alter the target sequence...

...Being an entrepreneur is difficult; it requires an immense amount of self-knowledge and an unwavering...become something tangible.  When you think of entrepreneurship in the 21st century, who do you think of?  ...server space. But what if you want to be an entrepreneur in the health sciences?  Is this a different...with innovative ideas in all facets of the entrepreneurial process in order to develop those ideas into...lessons I learned while becoming a health science entrepreneur at 22. Start with your network You've spent ...leave people remembering your potential as an entrepreneur, not your credentials. If you have an idea, ...

...When preparing to graduate from university, many students are confronted with the question ‘what now?...Michael Koeris' Journey from Grad Student to Entrepreneur Science careers divided by the most pertinent...actively require these skills, including: Science Reporter Scientific Sales Epidemiologist Scientific Recruitment... actively use these skills include: Science Reporter Scientific Illustrator Research and Development...desirable in a range of roles, including: Science Reporter Technical Writer Statistician Bioinformatician...Michael Koeris' Journey from Grad Student to Entrepreneur Learn to Network and Make the Contacts for Your...

...the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. Think...pitch a Hot Plasmids section (antibodies and viral preps welcome!) here. Here's what you'll find in this...ubiquitous promoter drives expression of an mCherry reporter.  After using mCherry for FACS sorting of the ... you the benefit of a polyclonal, with the reproducibility of a (recombinant) monoclonal. Another reason...observed high fluorescence resilience to these preparations. After crystalizing the hfYFP structure, the...

...ApoB-NanoLuc reporter by inserting the NanoLuc luciferase downstream of the apoB gene. Using this reporter, the...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...Cell, 2019. PubMed PMID: 30639098. LipoGlo: A reporter for measuring ApoB bound lipoproteins Article ... and localization in vivo. The LipoGlo system represents a new, intriguing approach to expand our understanding...cells. These changes were larger than previously reported for other organisms, and the Gottschalk group ...

...to primate neocortex. Cell Reports, 34(13). https://doi.org/10.1016/j.celrep.2021.108754 Ravindra Kumar...Look it up in Addgene’s Data Hub and the CLOVER repository of published systemic AAVs to find commonly used...removes, not just reduces, empty viral capsids in your prep can help reduce the risk of an immune response. ...primate. Many of the systemic capsids in our repository are deposited by the Gradinaru lab. You can visit...LY6C1) Stanton et al., 2023 * indicates viral preps available. Mechanisms of crossing the BBB are indicated...commonly requested systemic capsid in the Addgene repository. Like its parent AAV-PHP-B, it can be produced...region VIII of AAV9-X1.1 to that of AAV1. AAV1-X1 reproduces AAV9-X1.1’s brain endothelial cell-specific tropism...

...a subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These... a cleavage free manner. The dCas9-SSAP system reports an efficiency of up to 20%, comparable to commonly... For a one kilobase insertion, the researchers reported an editing error rate of less than 0.3%, both ...using mStrawberry, localization peptides, and reporters that drive the expression of the fluorescent protein...response element (TRE) or kinase translocation reporters (KTRs). Find visual barcode plasmids here! Kaufman...

...Figure 2: OCaMP structure and mechanism of action. Reproduced from Aggarwal et al. 2025 under a CC-BY-NC-ND...is 565 nm, and 2P excitation peak is 1050 nm. Reproduced from Aggarwal et al. 2025 under a CC-BY-NC-ND...and yellow GECIs measured with soluble protein. Reproduced from Aggarwal et al. 2025 under a CC-BY-NC-ND... APs in neurons expressing OCaMP or jRGECO1a. Reproduced from Aggarwal et al. 2025 under a CC-BY-NC-ND...Whether you're working with cell cultures, slice preparations, or in vivo imaging, there's a version that ...cultures, zebrafish, and mouse brain, check out the preprint on bioRxiv. {% module_block module "widget_221e8fe4...

...diagram depicting transcription, or DNA repair, or replication, or any number of CRISPR applications and...methylation status at a double-strand break that needs repairing. They may be looking at chromatin at the level...this list, that does not necessarily mean the repository does not have it. That said, if you know of a...plasmids available from Addgene Many articles reporting chromatin-related research describe the production...Lawrence JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small...

... program coordinator.   6. Virtual interview prep Prepare for your virtual interview just as you would...yourself. Be mindful that January and February represent peak season for internship applications. Thus ...that program of your dream.  “Chance favors the prepared mind” by Louis Pasteur These tips are a set of...of common sense approaches that will prepare you for the competitive landscape beyond college. They will...guarantee of success. Don’t luck into an internship, prepare the road ahead for success. Every student should...