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  1. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ... lines you are using, it is best to start from scratch. Obtain a vial of the desired cell line from a ...Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources...

  2. Plasmids 101: Control Plasmids

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    Blog Post
    ...positive control, the internal control vector, was described earlier. Once you are sure your conditions are...come from multiple, independent sources. We'll describe these a bit more below, but the papers cited in...

  3. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post
    ... from a gene synthesis company such as IDT or Genscript, and cloning them sequentially into the chosen...combining the AAV and CRISPR-based approaches is described in several recent reviews (Howes and Schofield...

  4. Five Popular Model Organisms

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    Blog Post
    ...difficult and time consuming. GAL4/UAS was first described in 1993 by Norbert Perrimon’s lab and has been...unicellular organisms, they can also be used to easily screen multiple therapeutic compounds in one experiment...

  5. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...science, a reviewer’s critical comments on your manuscript is a form of (hopefully constructive) conflict...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...

  7. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...) to remove mutated exons from the dystrophin transcript. Unfortunately, the oligonucleotides only modestly...in one experiment, they found the exon-skipped transcript constituted 59% of total dystrophin mRNA, similar...

  8. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...”, “colony”, etc. This will help save time by screening out irrelevant candidates, and, more importantly...importantly, it’ll help you define salary costs. 4. Ascribe a cost structure associated to these tools, skills...

  9. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...2025).In this latest work, the team designed and screened a large library of iGluSnFR3 mutants, identifying... insertion, a combined endonuclease-reverse transcriptase uses homology arms flanking an RNA payload to...

  10. Important Considerations When Using AAVs

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    Blog Post
    ... but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts from Addgene 1. Location...and can immediately undergo replication and transcription. However, with scAAV the effective genome size...

  11. A History of Genome Engineering in Popular Culture

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    Blog Post
    ... appealing to researchers.  TALENs In 2010, transcription activator-like effector nucleases (TALENs) were... which is a fusion between Cas9 and reverse transcriptase. Using an elongated template + guide RNA called...

  13. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    .... Bell, Charles C., et al. "A high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations...Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse Models with CRISPR/Cas9...

  15. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... tested and will help healthcare professional prescribe and identify potential beneficiaries. Collaborative...Collection Pages Plasmid Grant to Aid Cervical Cancer Screening in Ghana Modulate Cancer Signalling Resources...

  16. Typing CRISPR Systems

    Type
    Blog Post
    ...interference, as it can target and degrade select RNA transcripts, or be engineered to conduct direct RNA editing...-Aslan, A., & Marraffini, L. A. (2015). Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas...

  17. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...the CGBE architecture. They conducted a CRISPRi screen to identify endogenous genes that impact CGBE efficiency...•G-to-G•C base editors developed using CRISPRi screens, target-library analysis, and machine learning....

  18. New Antibody Animation!

    Type
    Blog Post
    ...different antibody isotypes found in mammals.      Subscribe to Addgene's YouTube channel for more helpful ...
Showing: 401 - 420 of 776 results