... image from Evan Amos.   After the transfer, some areas of the membrane remain unbound by protein....antibodies and labels being used and may require some trial and error (Yang et al., 2012).  Choosing your...expressed protein at physiologically relevant levels. Some vendors will also test their antibody in a variety...polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy...

... a forward or reverse orientation so you’ll need some way to verify that the insert ended up in the direction... plasmid) and plasmid backbone. Because you lose some DNA during the gel purification step, it is important...different migration speeds; after running the gel for some duration of time, these differently sized pieces...post or pre run in the table above, respectively). Some of the above stains require you to visualize your...

...Rocío López-Diego, & Zafira Castano Corsino. When someone says “internationalization” young hearts often ...talented professionals (who migrated from their country some years ago looking for their career improvement) ...with mentors who are working in USA and Europe, something that I would not able to do without a program ...Spanish scientists (MD, PhD and MD/PhD) established in some of the top Universities in USA and Europe (Harvard...

...into primary cells, this blog article will give you some tips to plan your experiment using the lentiviral...cells often relied on chemical (CaCl2, cationic liposome) or electrical (electric current forming membrane...and the marker can be separated by an internal ribosome entry site (IRES) and expressed under the same...supernatant in order to precipitate the viral particles. Some protocols suggest adding sucrose to the supernatant...

... the technology. In this blog, we will highlight some common MoClo applications and kits. Keep in mind...range of parts, including copy number machinery, chromosomal integration modules, and many more of the usual...conditional gene expression, multiplexed CRISPR, and chromosomal integration of larger expression cassettes (Shaw...addition to additional elements for S. cerevisiae chromosomal integration and gene expression, the system includes...

...antibodies can be produced for research purposes, some of which take advantage of natural immune system...of the antibody. Let’s dive in and take a look at some of these applications. Western blot Western blots...Enzyme-linked Immunosorbent Assay). Immunoprecipitation Sometimes, instead of just detecting the presence of protein... are referred to as functional grade antibodies. Some of the research and therapeutic uses overlap: antibodies...

...to as “Epi” illumination. Therefore these are sometimes call Epi-fluorescence microscopes. (See left image...cell cultures, small model embryos >15 μm Thsee are some of the most challenging samples to image as they...image thick scattering samples in their entirety, something that is not possible via traditional confocal ...and cleared tissues These samples always require some form of optical sectioning. Point scanning confocal...

...knockouts using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR...also like to see what you think so we’ll ask you some questions on Friday - share with us how you use ...CRISPR guide! If you’re not into Twitter, we have some exciting news for you too! The CRISPR field is always...

... with Addgene mugs and some delicious hot chocolate for the lab. Here are some of our 2017 contest winners...but our community of creative scientists delivered some spectacular photos once again this year! All winners...

...along the way to ourselves so we decided to share some of our tips in our new series: Addgene Lab Tips!...tip #1: Glove trick Putting on lab gloves can sometimes be annoying. To make it easier, try this quick... to keep up to date on the latest episode.  Have some suggestions for future tips?  Send your ideas to...

...research done in our depositor labs. Some are written by Addgenies and some are written by the depositors themselves...favorite posts from 2022! Wow! Our depositors do some really incredible science - I hope you've enjoyed...

...advantage of repair pathways within the cell — in some cases, the same repair pathways that were considered...Surprisingly, the team noticed that this editor sometimes introduced C → G edits at certain positions within...multi-step cellular repair pathway that is known for sometimes causing double-strand breaks (DSBs). The Fei-Long...common intermediate of CGBE activity, and they are sometimes processed by error-prone non-homologous end joining...

...the loading and unloading of the column and give some tips for performing this purification.  Check out...titration by qPCR using SYBR green technology Here’s some advice on how to successfully set up your qPCR to... new to tissue culture, check out this video for some tips and best practices for getting started with...

...these plasmid deposits came in this year, there were some plasmids deposited as early as 2006 that have helped...your science along? We’ll also take a look back on some fascinating Addgene history that you might not have... into 2021. Check out the infographic below for some Addgene depositor fun facts for a quick way to grasp...

...content teams have made some changes to the look and feel of addgene.org. Some you’ll notice right away...Starting today, you may notice some changes to our website! Part of sharing science and making access...

...colleague. She mentioned Addgene was looking for someone with HR expertise to join their newly formed compensation... and also serves as an objective body to oversee some of Addgene’s compensation decisions. It was a great... enough people will be vaccinated and we’ll have some herd immunity, which raises questions like “How ...

...transfection. The 2A "self cleaving" peptide causes the ribosome to translate Cas9-2A and the Puromycin resistance...the name? Not included in this plasmid's name are some additional features. The first and most imporant...Learn about Using CRISPR to Generate Knockouts Read Some Common Ways to Deliver CRISPR Plasmids to Mammalian...

... perturbations. So, while some might argue that the only way to do something is to do it huge, there are...and the assay are just as critical for success. Sometimes, you might have a few positive control genes on...

...an indication of the stiff competition or says something about my cooking.   This is a creative (and delicious...competitions in the future, and hopefully include some of our favorite winning recipes. Hack Day lunches...Director, Joanne Kamens, responded by starting something we call “Random Lunches.” Once or twice a year...

...Plasmid Collection for “Functional Annotation of Somatic Mutations in Cancer.” Of all the mutations and ...how a given gene is involved in a complex pathway. Some of the most heavily-studied genes in cancer research...Kwok-Shing, et al. "Systematic functional annotation of somatic mutations in cancer." Cancer Cell 33.3 (2018): ...